Journal of Integrative Agriculture ›› 2023, Vol. 22 ›› Issue (7): 2126-2137.DOI: 10.1016/j.jia.2022.10.006

• • 上一篇    下一篇

SsRSS1基因参与甘蔗鞭黑粉菌的水杨酸响应及致病性调控

  

  • 收稿日期:2022-06-24 接受日期:2022-09-05 出版日期:2023-07-20 发布日期:2022-09-05

SsRSS1 mediates salicylic acid tolerance and contributes to virulence in sugarcane smut fungus

ZHANG Hao-yang1, YANG Yan-fang1, GUO Feng2, SHEN Xiao-rui2, LU Shan1, 2, 3#, CHEN Bao-shan1, 2, 3#   

  1. 1 College of Agriculture/Guangxi Key Laboratory of Sugarcane Biology, Guangxi University, Nanning 530004, P.R.China
    2 State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/College of Life Science and Technology,
    Guangxi University, Nanning 530004, P.R.China
    3 Ministry and Province Co-sponsored Collaborative Innovation Center for Sugarcane and Sugar Industry, Guangxi University,
    Nanning 530004, P.R.China
  • Received:2022-06-24 Accepted:2022-09-05 Online:2023-07-20 Published:2022-09-05
  • About author:ZHANG Hao-yang, E-mail: 1817304024@st.gxu.edu.cn; #Correspondence CHEN Bao-shan, Tel: +86-771-3225260, E-mail: chenyaoj@gxu.edu.cn; LU Shan, Tel: +86-771-3394554, E-mail: lushan@gxu.edu.cn
  • Supported by:
    This study was supported by the grants from the National Natural Science Foundation of China (31872635), the Guangxi Key Laboratory of Sugarcane Biology, China (2018-266-Z01), and the Department of Science and Technology of Guangxi Zhuang Autonomous Region, China (AD17129002).

PDF

摘要：

【目的】甘蔗鞭黑粉菌引起的甘蔗黑穗病是甘蔗上最严重的系统性真菌病害，对全球甘蔗生产造成严重的损失。然而，该病原菌的致病机制在很大程度上仍然未知，对甘蔗鞭黑粉菌致病性调控机制的研究将有助于开发新的病害防治策略。【方法】利用农杆菌T-DNA介导的CRISPR-Cas9基因插入突变及回补系统分别在甘蔗鞭黑粉菌两个不同的交配型担孢子中构建水杨酸感知调节因子SsRss1的敲除株和回补株。通过对细胞表型观察、生长速度、压力应激、水杨酸耐受性、有性配合以及致病的检测，明确SsRSS1基因在甘蔗鞭黑粉菌重要生物过程中的功能。【结果】SsRss1是一个具有保守锌簇DNA结合结构域的GAL4样转录因子，受水杨酸诱导表达；敲除SsRss1编码基因SsRSS1不影响甘蔗鞭黑粉菌单倍体担孢子的细胞形态、体外生长速率及有性配合能力，但降低了单倍体担孢子对水杨酸的耐受力；SsRSS1缺失突变体对甘蔗的侵染率下降约40%，受侵染植株的黑鞭症状形成时间延迟或植未能形成黑鞭；SsRSS1基因缺失不影响植株体内菌丝的形态及冬孢子的形成与萌发。【结论】甘蔗鞭黑粉菌SsRss1参与病原菌对水杨酸的响应并对水杨酸的耐受性和致病性有明显贡献。【创新性】本研究首次报道了甘蔗鞭黑粉菌转录因子SsRss1在致病性调控方面的作用及机制，加深了该病原菌与甘蔗互作机理的认识。

Abstract: Sugarcane smut caused by Sporisorium scitamineum is a destructive disease responsible for significant losses in sugarcane production worldwide. However, the mechanisms underlying the pathogenicity of this fungus remain largely unknown. In this study, we found that the disruption of the SsRSS1 gene, which encodes a salicylic acid (SA) sensing regulator, does not affect phenotypic traits such as the morphology, growth rate, and sexual mating ability of haploid basidiospores, but rather reduces the tolerance of basidiospores to SA stress by blocking the induction of SsSRG1, a gene encoding a SA response protein in S. scitamineum. SsRSS1 deletion resulted in the attenuation of the virulence of the fungus. In addition to a significant reduction in whip formation, a portion of plantlets (18.3%) inoculated with the ΔSsRSS1 strains were found to be infected but failed to produce whips for up to 90 days post-inoculation. However, the development of hyphae and teliospore from the ΔSsRSS1-infected plants that formed whips seemed indistinguishable from that in the wild-type-infected plants. Combined, our findings suggested that SsRss1 is required for maintaining fungal fitness in planta by counteracting SA stress.

Key words: Sporisorium scitamineum ,  SsRSS1 ,  pathogenicity ,  salicylic acid respons