外源氨基酸应用促进了盛花期棉铃蛋白质合成和碳水化合物转化从而提高了棉铃Bt蛋白浓度和皮棉产量

doi:10.1016/j.jia.2022.10.003

Journal of Integrative Agriculture ›› 2023, Vol. 22 ›› Issue (6): 1684-1694.DOI: 10.1016/j.jia.2022.10.003

外源氨基酸应用促进了盛花期棉铃蛋白质合成和碳水化合物转化从而提高了棉铃Bt蛋白浓度和皮棉产量

收稿日期:2022-04-08 修回日期:2022-10-04 接受日期:2022-09-01 出版日期:2023-06-20 发布日期:2022-09-01

Enhancing boll protein synthesis and carbohydrate conversion by the application of exogenous amino acids at the peak flowering stage increased the boll Bt toxin concentration and lint yield in cotton

LIU Zhen-yu¹, LI Yi-yang¹, Leila. I. M. TAMBEL², LIU Yu-ting¹, DAI Yu-yang¹, XU Ze¹, LENG Xin-hua¹, ZHANG Xiang¹, CHEN De-hua^1#, CHEN Yuan^1#

¹Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, P.R.China
²Agricultural Research Cooperation, Biotechnology and Biosafety Research Center, Khartoum 13314, Sudan

Received:2022-04-08 Revised:2022-10-04 Accepted:2022-09-01 Online:2023-06-20 Published:2022-09-01
About author:LIU Zhen-yu, Tel: +86-514-87979357, E-mail: 1127317278@qq.com; #Correspondence CHEN De-hua, Tel: +86-514-87979357, E-mail: cdh@yzu.edu.cn; CHEN Yuan, Tel: +86-514-87979357, E-mail: yuanchen@yzu.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (31901462 and 31671613), the Natural Science Foundation of Jiangsu Province, China (BK20191439), the Postgraduate Research & Practice Innovation Program of Jiangsu Province, China (KYCX22_3508) and the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (PAPD).

摘要/Abstract

摘要：

与其他器官相比，Bt转基因棉棉铃中杀虫蛋白含量最低。该文研究了盛花期喷施氨基酸对棉铃Bt蛋白浓度和产量形成的影响、相关的蛋白质合成和碳水化合物转化机制。2017-2018年棉花生长季节，以2个陆地棉品种（杂交种泗抗3号和常规种泗抗1号）为试验材料，设置三个处理（即CK，对照；LA1，组成Bt蛋白的5种主要氨基酸；LA2，21种氨基酸）于盛花期喷施棉铃。结果表明，棉铃Bt蛋白浓度提高5.2%-16.4%，籽棉产量提高5.5%-11.3%，2个氨基酸处理间没有差异。此外，由于氨基酸的应用，棉铃游离氨基酸和可溶性蛋白含量、谷氨酸丙酮酸转氨酶（GPT）和谷氨酸草酰乙酸转氨酶（GOT）活性、葡萄糖和果糖含量、可溶性酸性转化酶（SAI）活性均有所增加。同时发现，棉铃Bt蛋白含量、铃数增长量和铃重与碳氮代谢密切相关，Bt蛋白含量与游离氨基酸含量、可溶性蛋白含量呈显著以上水平线性正相关。花后15-25天，铃数增长量与GPT活性、GOT活性呈显著以上水平线性正相关。花后55-65日铃重与SAI活性呈显著以上水平线性正相关。这些结果表明，氨基酸的应用促进了棉铃蛋白质合成和碳水化合物转化，从而提高了棉铃Bt蛋白浓度和皮棉产量。

Abstract:

In Bacillus thuringenesis (Bt) transgenic cotton, the cotton boll has the lowest insecticidal protein content when compared to the other organs. The present study investigated the effects of amino acid spray application at the peak flowering stage on the cotton boll Bt toxin concentration and yield formation. Boll protein synthesis and carbohydrate conversion were also studied to reveal the fundamental mechanism. Three treatments (i.e., CK, the untreated control; LA1, five amino acids; LA2, 21 amino acids) were applied to two Bt cultivars of G. hirsutum (i.e., the hybrid Sikang 3 and the conventional Sikang 1) in the cotton-growing seasons during 2017 and 2018. Amino acid spray application at the peak flowering stage resulted in an increase of 5.2–16.4% in the boll Bt protein concentration and an increase of 5.5–11.3% in the seed cotton yield, but there was no difference between the two amino acid treatments. In addition, amino acid applications led to increases in the amino acid content, soluble protein content, glutamate pyruvate transaminase (GPT) activity, glutamate oxaloacetate transaminase (GOT) activity, glucose content, fructose content and soluble acid invertase (SAI) activity. This study also found that Bt protein content, enhanced boll number and the weight of opened bolls were closely related to carbon and nitrogen metabolism. The Bt protein content had significant linear positive correlations with amino acid and soluble protein contents. Enhanced boll number had significant linear positive correlations with the GPT and GOT activities from 15–25 days after flowering (DAF). The weight of opened bolls from 55–65 DAF had a significant linear positive correlation with the SAI activity. These results indicate that the enhancement of boll protein synthesis and carbohydrate conversion by amino acid application resulted in a simultaneous increase in the boll Bt protein concentration and cotton lint yield.

Key words: Bt cotton , boll insecticidal protein , protein synthesis , carbohydrate conversion

LIU Zhen-yu, LI Yi-yang, Leila. I. M. TAMBEL, LIU Yu-ting, DAI Yu-yang, XU Ze, LENG Xin-hua, ZHANG Xiang, CHEN De-hua, CHEN Yuan. 外源氨基酸应用促进了盛花期棉铃蛋白质合成和碳水化合物转化从而提高了棉铃Bt蛋白浓度和皮棉产量[J]. Journal of Integrative Agriculture, 2023, 22(6): 1684-1694.

LIU Zhen-yu, LI Yi-yang, Leila. I. M. TAMBEL, LIU Yu-ting, DAI Yu-yang, XU Ze, LENG Xin-hua, ZHANG Xiang, CHEN De-hua, CHEN Yuan. Enhancing boll protein synthesis and carbohydrate conversion by the application of exogenous amino acids at the peak flowering stage increased the boll Bt toxin concentration and lint yield in cotton[J]. Journal of Integrative Agriculture, 2023, 22(6): 1684-1694.

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外源氨基酸应用促进了盛花期棉铃蛋白质合成和碳水化合物转化从而提高了棉铃Bt蛋白浓度和皮棉产量

Enhancing boll protein synthesis and carbohydrate conversion by the application of exogenous amino acids at the peak flowering stage increased the boll Bt toxin concentration and lint yield in cotton

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