JIA-2021-0361普通小麦籽粒灌浆速率及相关性状的全基因组连锁和关联分析

doi:10.1016/j.jia.2022.07.032

Journal of Integrative Agriculture ›› 2022, Vol. 21 ›› Issue (10): 2805-2817.DOI: 10.1016/j.jia.2022.07.032

所属专题：麦类遗传育种合辑Triticeae Crops Genetics · Breeding · Germplasm Resources

JIA-2021-0361普通小麦籽粒灌浆速率及相关性状的全基因组连锁和关联分析

收稿日期:2021-02-25 接受日期:2021-07-06 出版日期:2022-10-01 发布日期:2021-07-06

Genetic dissection of the grain filling rate and related traits through linkage analysis and genome-wide association study in bread wheat

YU Hai-xia^1*, DUAN Xi-xian^1*, SUN Ai-qing¹, SUN Xiao-xiao¹, ZHANG Jing-juan², SUN Hua-qing³, SUN Yan-yan¹, NING Tang-yuan¹, TIAN Ji-chun¹, WANG Dong-xue¹, LI Hao¹, FAN Ke-xin¹, WANG Ai-ping², MA Wu-jun⁴, CHEN Jian-sheng¹

¹State Key Laboratory of Crop Biology/Key Laboratory of Crop Water Physiology and Drought-tolerance Germplasm Improvement, Minstry of Agriculture and Rural Affairs/Group of Wheat Quality Breeding, College of Agronomy, Shandong Agricultural University, Tai’an 271018, P.R.China
²Dezhou Agricultural Protection and Technological Extension Center, Dezhou 253000, P.R.China
³Zhongnong Tiantai Seed Co., Ltd., Pingyi 273300, P.R.China
⁴School of Veterinary and Life Sciences, Murdoch University, Murdoch, WA 6150, Australia

Received:2021-02-25 Accepted:2021-07-06 Online:2022-10-01 Published:2021-07-06
About author:YU Hai-xia, E-mail: yuhaixia66@163.com; DUAN Xi-xian, E-mail: 1278901428@qq.com; Correspondence CHEN Jian-sheng, Tel: +86-538-8241959, E-mail: jshch@sdau.edu.cn; MA Wu-jun, E-mail: W.Ma@murdoch.edu.au * These authors contributed equally to this study.
Supported by:
This work was supported by the National Natural Science Foundation of China (31971936) and the Science &Technology Projects of Shandong Province, China (2019YQ028, 2020CXGC010805, 2019B08, 2019YQ014 and ZR2020MC093).

摘要/Abstract

摘要： 籽粒灌浆速率（GFR）在小麦产量形成过程中起着关键作用，但由于表型调查困难等原因，对其遗传解析研究甚少。本研究测定了1个重组自交系群体和1个自然群体籽粒灌浆性状，基于高密度图谱进行相关性状的连锁分析和全基因组关联分析。在染色体 1B、4B和5A上鉴定到17个稳定的QTLs。其中IWB19555-IWB56078 连锁区间对性状 GFR₁、GFR_max、KL、KW、KT 和TKW具有多效性，表型变异解释率（PVE）为13.38%（KW）- 33.69%（TKW）。检测到198个显著性状关联位点（MTAs）分布在除了3D和4D之外的染色体上。GFR的主要关联位点包括 IWB44469（11.27%）、IWB8156（12.56%）和IWB24812（14.46%）。检测到IWB41019是籽粒大小相关的重要多效性位点。通过GWAS鉴定到的IWB35850与连锁分析获得的QGFR_max2B.3-11位于同一区域，该区域包含两个高置信候选基因。检测到两个重要的粒重相关 QTL与灌浆速率 QTL定位到同一区间。这些发现有助于解析 GFR 的遗传基础，为小麦产量性状 QTL候选基因预测提供理论依据。

Abstract:

Wheat grain yield is generally sink-limited during grain filling. The grain-filling rate (GFR) plays a vital role but is poorly studied due to the difficulty of phenotype surveys. This study explored the grain-filling traits in a recombinant inbred population and wheat collection using two highly saturated genetic maps for linkage analysis and genome-wide association study (GWAS). Seventeen stable additive quantitative trait loci (QTLs) were identified on chromosomes 1B, 4B, and 5A. The linkage interval between IWB19555 and IWB56078 showed pleiotropic effects on GFR₁, GFR_max, kernel length (KL), kernel width (KW), kernel thickness (KT), and thousand kernel weight (TKW), with the phenotypic variation explained (PVE) ranging from 13.38% (KW) to 33.69% (TKW). 198 significant marker-trait associations (MTAs) were distributed across most chromosomes except for 3D and 4D. The major associated sites for GFR included IWB44469 (11.27%), IWB8156 (12.56%) and IWB24812 (14.46%). Linkage analysis suggested that IWB35850, identified through GWAS, was located in approximately the same region as QGFRmax2B.3-11, where two high-confidence candidate genes were present. Two important grain weight (GW)-related QTLs colocalized with grain-filling QTLs. The findings contribute to understanding the genetic architecture of the GFR and provide a basic approach to predict candidate genes for grain yield trait QTLs.

Key words: wheat , grain-filling rate , linkage analysis , genome-wide association study

. JIA-2021-0361普通小麦籽粒灌浆速率及相关性状的全基因组连锁和关联分析[J]. Journal of Integrative Agriculture, 2022, 21(10): 2805-2817.

YU Hai-xia, DUAN Xi-xian, SUN Ai-qing, SUN Xiao-xiao, ZHANG Jing-juan, SUN Hua-qing, SUN Yan-yan, NING Tang-yuan, TIAN Ji-chun, WANG Dong-xue, LI Hao, FAN Ke-xin, WANG Ai-ping, MA Wu-jun, CHEN Jian-sheng. Genetic dissection of the grain filling rate and related traits through linkage analysis and genome-wide association study in bread wheat[J]. Journal of Integrative Agriculture, 2022, 21(10): 2805-2817.

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JIA-2021-0361普通小麦籽粒灌浆速率及相关性状的全基因组连锁和关联分析

Genetic dissection of the grain filling rate and related traits through linkage analysis and genome-wide association study in bread wheat

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