Journal of Integrative Agriculture ›› 2022, Vol. 21 ›› Issue (8): 2413-2421.DOI: 10.1016/S2095-3119(21)63753-X

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谷氨酰胺转录调控玉米根铵转运蛋白ZmAMTs基因表达

  


  • 收稿日期:2021-03-18 接受日期:2021-05-19 出版日期:2022-08-01 发布日期:2021-05-19

Ammonium-dependent regulation of ammonium transporter ZmAMT1s expression conferred by glutamine levels in roots of maize

HUI Jing*, LIU Zhi*, DUAN Feng-ying, ZHAO Yang, LI Xue-lian, AN Xia, WU Xiang-yu, YUAN Li-xing   

  1. College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, P.R.China
  • Received:2021-03-18 Accepted:2021-05-19 Online:2022-08-01 Published:2021-05-19
  • About author:HUI Jing, E-mail: 1244508876@qq.com; Correspondence YUAN Li-xing, Tel: +86-10-62734424, E-mail: yuanlixing@cau.edu.cn * These authors contributed equally to this study.
  • Supported by:
    This work was financially supported by the National Natural Science Foundation of China (31471934 and 30971863) and the Major Project of China on New Varieties of GMO Cultivation (2016ZX08003-005).

摘要:

本文利用玉米水培分根体系发现,局部供铵到缺氮根系可以显著诱导ZmAMT1;1aZmAMT1;3基因的表达水平。测定15N标记铵吸收速率与根组织氨基酸含量发现,铵诱导的ZmAMT1s基因表达与根系铵吸收能力及谷氨酰胺含量显著正相关。外界添加谷氨酰胺合成酶抑制剂MSX时,供铵则不能诱导缺氮根系中ZmAMT1;1aZmAMT1;3基因的表达,表明铵的同化产物谷氨酰胺,而不是铵本身,负责调控ZmAMT1s基因表达。此外,外界供应不同浓度谷氨酰胺到缺氮根系发现,较低浓度的谷氨酰胺就能够诱导ZmAMT1s基因表达,但高浓度谷氨酰胺却能够抑制其表达。以上研究结果表明,为了严格调节玉米根系铵吸收能力,铵转运蛋白ZmAMT1s基因在转录水平被严谨调控,与根内谷氨酰胺水平密切相关


Abstract:

In maize, two root epidermis-expressed ammonium transporters ZmAMT1;1a and ZmAMT1;3 play major roles in high-affinity ammonium uptake.  However, the transcriptional regulation of ZmAMT1s in roots for ensuring optimal ammonium acquisition remains largely unknown.  Here, using a split root system we showed that ZmAMT1;1a and ZmAMT1;3 transcript levels were induced by localized ammonium supply to nitrogen-deficient roots.  This enhanced expression of ZmAMT1s correlated with increases in 15NH4+ influx rates and tissue glutamine concentrations in roots.  When ammonium was supplied together with methionine sulfoximine, an inhibitor of glutamine synthase, ammonium-induced expression of ZmAMT1s disappeared, suggesting that glutamine rather than ammonium itself regulated ZmAMT1s expression.  When glutamine was supplied to nitrogen-deficient roots, expression levels of ZmAMT1s were enhanced, and negative feedback regulation could subsequently occur by supply of glutamine at a high level.  Thus, our results indicated an ammonium-dependent regulation of ZmAMT1s at transcript levels, and a dual role of glutamine was suggested in the regulation of ammonium uptake in maize roots.

Key words: ammonium transporter ,  pH ,  glutamine ,  maize roots ,  nitrogen ,  transcriptional regulation