The use of widely targeted metabolite profiling to reveal the senescence changes in postharvest ‘Red Globe’ (Vitis vinifera) grape berries

doi:10.1016/S2095-3119(21)63725-5

Journal of Integrative Agriculture ›› 2022, Vol. 21 ›› Issue (4): 1028-1043.DOI: 10.1016/S2095-3119(21)63725-5

收稿日期:2020-12-16 接受日期:2021-05-10 出版日期:2022-04-01 发布日期:2021-05-10

The use of widely targeted metabolite profiling to reveal the senescence changes in postharvest ‘Red Globe’ (Vitis vinifera) grape berries

XU Teng-fei^1*, YANG Xin^2*, ZHANG Meng², GUO Shui-huan², FU Wen-jing², ZHOU Bi-jiang², LIU Yu-jia², MA Hai-jun³, FANG Yu-lin², YANG Gang⁴, MENG Jiang-fei²

¹College of Horticulture, Northwest A&F University, Yangling 712100, P.R.China
²College of Enology, Northwest A&F University, Yangling 712100, P.R.China
³ College of Biological Science and Engineering, North Minzu University, Yinchuan 750021, P.R.China
⁴Shenzhen Camartina Wine Co., Ltd., Shenzhen 518031, P.R.China

Received:2020-12-16 Accepted:2021-05-10 Online:2022-04-01 Published:2021-05-10
About author:XU Teng-fei, E-mail: tengfei.xu@nwafu.edu.cn; Correspondence YANG Gang, E-mail: weitongwine@hotmail.com; MENG Jiang-fei, E-mail: mjfwine@nwafu.edu.cn * These authors contributed equally to this study.
Supported by:
This work was supported by the Natural Science Foundation of China (31801833 and 31801811), the Innovation Capability Support Programs of Shaanxi Province, China (2020KJXX-035), the China Postdoctoral Science Foundation (2019M653771 and 2019T120953), the Fundamental Research Funds for the Central Universities of China (2452019016), and the China Agriculture Research System of MOF and MARA (CARS-29-zp-6).

摘要/Abstract

摘要：

鲜食葡萄果实在采后衰老过程中代谢物会发生一系列变化。本研究的目的是利用基于UPLC-MS/MS的广泛靶向代谢组技术分析鲜食葡萄红地球在不同的衰老阶段代谢物差异。结果显示共鉴定出135种差异代谢物。在采后衰老过程中，大部分差异类黄酮（如花葵素3-O-葡萄糖苷、槲皮素3-O-葡萄糖苷和花青素3-O-葡萄糖苷）和L-天冬氨酸含量下降，而酚酸（如反式4-羟基肉桂酸甲酯）和泛醇含量升高。在衰老的前期，大部分差异脂质（尤其是溶血蛋白），核苷酸及其衍生物（如尿苷）的水平降低，但是在衰老后期这些代谢物的含量又升高。基于此，这些结果将为合理控制鲜食葡萄果实的采后衰老过程提供了指导，为进一步研究采后葡萄的衰老机制奠定坚实的基础。

Abstract: Changes in the metabolites of table grapes (Vitis vinifera) reportedly occur during postharvest senescence. The aim of this study was to determine the metabolomic differences in postharvest table grapes (‘Red Globe’) after being subjected to different senescence periods. To this end, we used widely targeted metabolomics based on ultra-performance liquid chromatography and tandem mass spectrometry. A total of 135 differential metabolites were identified. During postharvest senescence, the levels of most differential flavonoids (e.g., pelargonidin 3-O-glucoside, quercetin-3-O-glucoside, and cyanidin 3-O-glucoside) and L-aspartic acid decreased, while the levels of phenolic acids (e.g., trans-4-hydroxycinnamic acid methyl ester) and pantothenol increased. During early and late senescence, the levels of most differential lipids, especially LysoPC, as well as those of nucleotides and their derivatives, such as uridine, decreased and increased, respectively. Collectively, the findings of this study provide fundamental insights into the reasonable control of table grape fruit postharvest senescence and lay a solid foundation for further research.

Key words: table grape , berry , postharvest senescence , widely targeted metabolites

. [J]. Journal of Integrative Agriculture, 2022, 21(4): 1028-1043.

XU Teng-fei, YANG Xin, ZHANG Meng, GUO Shui-huan, FU Wen-jing, ZHOU Bi-jiang, LIU Yu-jia, MA Hai-jun, FANG Yu-lin, YANG Gang, MENG Jiang-fei. The use of widely targeted metabolite profiling to reveal the senescence changes in postharvest ‘Red Globe’ (Vitis vinifera) grape berries[J]. Journal of Integrative Agriculture, 2022, 21(4): 1028-1043.

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The use of widely targeted metabolite profiling to reveal the senescence changes in postharvest ‘Red Globe’ (Vitis vinifera) grape berries

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