Differences of aroma development and metabolic pathway gene expression between Kyoho and 87-1 grapes

doi:10.1016/S2095-3119(20)63481-5

摘要/Abstract

摘要：

在本研究中，我们采用固相微萃取SPME结合气相质谱联用技术比较了这两个葡萄品种果实发育过程中香气组分和含量的差异，并通过荧光定量PCR法分析了香气合成途径，比如LOX-HPL、MEP和MVA，酶编码基因的表达差异。结果发现巨峰果实成熟过程中共检测到12种酯类香气物质，并且主要在转色后含量丰富，但87-1葡萄中没有检测到酯类香气物质；87-1葡萄果实中检测到了14种萜烯类香气物质，含量丰富，并以里那醇为主，但在巨峰果实中仅检测到少量的萜烯类香气物质；荧光定量PCR的结果表明醇酰基转移酶编码基因VvAAT的低表达可能是87-1葡萄酯类香气含量低的主要原因，而MEP代谢途径酶编码基因的低表达，特别是里那醇合成酶编码基因VvPNLinNer1的低表达可能是巨峰葡萄中萜烯类香气物质含量低的原因。本研究将有助于对葡萄香气代谢机理认识的加深，并为葡萄香气品质改良提供理论指导。

Abstract:

Aroma is an important quality trait of grapes and often the focus of consumers, viticulturists and grapevine breeders. Kyoho is a hybrid between Vitis vinifera and Vitis labrusca with a strawberry-like scent, while 87-1 is an early-ripening mutant of Muscat hamburg, belonging to Vitis vinifera, with a rose scent. In this study, we compared their aroma compositions and concentrations during berry development by headspace-SPME combined with gas chromatography-mass spectrometry (GC-MS), and analyzed the expression differences of enzyme-encoding genes in the LOX-HPL, MEP and MVA metabolic pathways by qRT-PCR. Twelve esters were detected in Kyoho during the whole berry development and they were abundant after veraison, but no esters were detected in 87-1 berries. Linalool was the dominant terpene among the 14 terpenes detected in 87-1 berries, while limited amounts of terpenes were detected in Kyoho berries. qRT-PCR analysis indicated that the low expression of VvAAT might explain the low content of ester volatiles in 87-1 berries, and the low expression of coding genes in the MEP pathway, especially VvPNLinNer1, might be the reason for the low content of volatile terpenes in Kyoho berries. The results from this work will promote our understanding of aroma metabolic mechanisms of grapes, and offer some suggestions for grape aromatic quality improvement.

Key words: grape , Kyoho , 87-1 , aroma , LOX-HPL , MEP , gene expression

. [J]. Journal of Integrative Agriculture, 2021, 20(6): 1525-1539.

JI Xiao-hao, WANG Bao-liang, WANG Xiao-di, WANG Xiao-long, LIU Feng-zhi, WANG Hai-bo. Differences of aroma development and metabolic pathway gene expression between Kyoho and 87-1 grapes[J]. Journal of Integrative Agriculture, 2021, 20(6): 1525-1539.

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