Expression profiles of Cry1Ab protein and its insecticidal efficacy against the invasive fall armyworm for Chinese domestic GM maize DBN9936

doi:10.1016/S2095-3119(20)63475-X

Journal of Integrative Agriculture ›› 2021, Vol. 20 ›› Issue (3): 792-803.DOI: 10.1016/S2095-3119(20)63475-X

收稿日期:2020-06-24 出版日期:2021-03-01 发布日期:2021-02-18

Expression profiles of Cry1Ab protein and its insecticidal efficacy against the invasive fall armyworm for Chinese domestic GM maize DBN9936

LIANG Jin-gang¹, ZHANG Dan-dan², LI Dong-yang^{1, 3}, ZHAO Sheng-yuan², WANG Chen-yao¹, XIAO Yu-tao⁴, XU Dong⁵, YANG Yi-zhong⁶, LI Guo-ping⁷, WANG Li-li⁸, GAO Yu², YANG Xue-qing⁹, YUAN Hai-bin¹⁰, LIU Jian¹¹, ZHANG Xiu-jie¹, WU Kong-ming²

¹ Development Center of Science and Technology, Ministry of Agriculture and Rural Affairs, Beijing 100176, P.R.China
² State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
³ State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, P.R.China
⁴ Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, P.R.China
⁵ Institute of Plant Protection and Soil Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, P.R.China
⁶ College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, P.R.China
⁷ Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R.China
⁸ Yantai Academy of Agricultural Sciences, Yantai 265500, P.R.China
⁹ College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, P.R.China
¹⁰ College of Plant Protection, Jilin Agricultural University, Changchun 130118, P.R.China
¹¹ College of Agriculture, Northeast Agricultural University, Harbin 150030, P.R.China

Received:2020-06-24 Online:2021-03-01 Published:2021-02-18
Contact: Correspondence WU Kong-ming, Tel: +86-10-82105551, E-mail: wukongming@caas.cn; ZHANG Xiu-jie, Tel: +86-10-59198155, E-mail: zhxj7410@sina.com
About author:LIANG Jin-gang, Tel: +86-10-59198125, E-mail: liangjingang@agri.gov.cn;
Supported by:
This work was funded by the National Genetically Modified Organism New Variety Breeding Program of China (2019ZX08012-004). We are grateful to Prof. Li Yunhe from Institute of Plant Protection, Chinese Academy of Agricultural Sciences for his helpful comments and suggestions.

摘要/Abstract

摘要：

草地贪夜蛾自2018年12月11日入侵云南后已在我国定殖并形成了周年繁殖区，春夏季将以周年繁殖区为起点向北迁飞。明确其周年发生区域，对指导我国草地贪夜蛾的监测预警及科学防控工作具有重要意义。对冬季草地贪夜蛾在我国热带和亚热带地区的种群动态研究表明，幼虫虫口密度与冬季温度显著正相关，热带地区草地贪夜蛾幼虫种群密度最高，其次为南亚热带地区，而中亚热带北部地区和北亚热带地区未见草地贪夜蛾幼虫发生。研究结果指出草地贪夜蛾在我国1月份等温线10℃以南的热带和南亚热带地区可周年繁殖，该区域包括海南省和台湾省，以及福建、广东、广西、贵州和云南五省的南部区域。该研究明确了草地贪夜蛾的在中国的周年繁殖区域，为该虫区域性监测预警及防控提供了重要依据。

Abstract:

The fall armyworm (FAW) Spodoptera frugiperda, which originated in the Americas, is advancing across China and threatening the nation’s maize crops. Currently, one widely used tool for its control is genetically modified (GM) Bacillus thuringiensis (Bt) maize. Sufficient content of Bt protein in appropriate plant parts is crucial for enhancing resistance against insect pests. In this study, we conducted a systematic investigation of Cry1Ab levels in Chinese domestic GM maize DBN9936, which has recently obtained a biosafety certificate, and evaluated its efficacy against FAW. Quantification of expression levels of Cry1Ab, via ELISA, indicated a spatio-temporal dynamic, with significant variation of mean Cry1Ab, ranging from 0.76 to 8.48 μg g^–1 FW with the Cry1Ab protein level ranked as: V6–V8 leaf>R1 leaf>R4 leaf>R1 silk>VT tassel>R4 kernel. Among the nine locations, the Cry1Ab levels in DBN9936 of the Xinxiang, Langfang, and Harbin fields were significantly lower than those from Wuhan and Shenyang, and were slightly, but not significantly lower than those from the other four fields. Furthermore, the artificial diet–Cry1Ab mixture and plant tissue feeding bioassays revealed that DBN9936 has high efficacy against FAW. The insecticidal efficacy of different tissues against FAW larvae reached 34–100% with a descending order of lethality as follows: VT leaf>R4 leaf>R1 husk>R1 silk>VT tassel>R4 kernel. Taken together, our results showed that Bt-Cry1Ab maize DBN9936 has potential as a promising strategy to manage FAW.

Key words: fall armyworm , genetically modified maize , DBN9936 , Cry1Ab expression , control efficacy

. [J]. Journal of Integrative Agriculture, 2021, 20(3): 792-803.

LIANG Jin-gang, ZHANG Dan-dan, LI Dong-yang, ZHAO Sheng-yuan, WANG Chen-yao, XIAO Yu-tao, XU Dong, YANG Yi-zhong, LI Guo-ping, WANG Li-li, GAO Yu, YANG Xue-qing, YUAN Hai-bin, LIU Jian, ZHANG Xiu-jie, WU Kong-ming . Expression profiles of Cry1Ab protein and its insecticidal efficacy against the invasive fall armyworm for Chinese domestic GM maize DBN9936[J]. Journal of Integrative Agriculture, 2021, 20(3): 792-803.

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Expression profiles of Cry1Ab protein and its insecticidal efficacy against the invasive fall armyworm for Chinese domestic GM maize DBN9936

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