Fingerprinting 146 Chinese chestnut (Castanea mollissima Blume) accessions and selecting a core collection using SSR markers

doi:10.1016/S2095-3119(20)63400-1

Journal of Integrative Agriculture ›› 2021, Vol. 20 ›› Issue (5): 1277-1286.DOI: 10.1016/S2095-3119(20)63400-1

所属专题：园艺-分子生物合辑Horticulture — Genetics · Breeding

收稿日期:2020-01-06 出版日期:2021-05-01 发布日期:2021-04-12

Fingerprinting 146 Chinese chestnut (Castanea mollissima Blume) accessions and selecting a core collection using SSR markers

NIE Xing-hua^{1, 2}, WANG Ze-hua², LIU Ning-wei², SONG Li², YAN Bo-qian², XING Yu^{1, 2}, ZHANG Qing², FANG Ke-feng³, ZHAO Yong-lian⁴, CHEN Xin⁵, WANG Guang-peng⁶, QIN Ling^{1, 2}, CAO Qing-qin^{1, 2}

¹Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture, Beijing 102206, P.R.China
² College of Plant Science and Technology, Beijing Key Laboratory for Agricultural Application and New Technique, Beijing University of Agriculture, Beijing 102206, P.R.China
³ College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, P.R.China
⁴ Chestnut Technology Experiment and Extension Station of Huairou District, Beijing 102206, P.R.China
⁵ Shandong Institute of Pomology, Shandong Academy of Agricultural Sciences, Tai’an 271000, P.R.China
⁶ Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, Changli 066600, P.R.China

Received:2020-01-06 Online:2021-05-01 Published:2021-04-12
Contact: Correspondence QIN Ling, Tel/Fax: +86-10-80797229, E-mail: qinlingbac@126.com; CAO Qing-qin, E-mail: caoqingqin@sina.com
About author:NIE Xing-hua, E-mail: niexinghuabua@163.com;
Supported by:
This work was supported by grants from the Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality, China (IDHT20180509); the National Key Research & Development Program of China (2018YFD1000605) and the Opening Project of Beijing Key Laboratory of New Technology in Agricultural Application, China (kf2018024).

摘要/Abstract

摘要：

中国板栗是世界上重要的坚果树种。我国板栗种质资源十分丰富，但在其资源利用与保护方面仍受到很大的限制。本研究利用18个荧光SSR标记对146份板栗资源进行了指纹图谱的构建，并解析了板栗品种群间的亲缘关系和筛选了板栗资源的核心种质。结果表明，每个基因座的平均等位基因数(Na)和多态性信息含量(PIC)分别为8.100和0.622，18个SSR标记表现出高的多态性。利用这些高效的标记，本试验成功构建了146份板栗资源的有唯一匹配的指纹图谱，并从中筛选出了7个SSR标记作为核心标记，实现了板栗资源的快速鉴定。对5个板栗品种群遗传关系进行研究，可知，中国板栗品种群被划分为3个类群，分别是类群I(华北品种群和西北品种群)、类群II(长江中下游品种群)和类群III(西南品种和东南品种群)。最后，我们选取了具有代表性的45个中国板栗资源作为核心种质。本研究为板栗资源的鉴定和品种群的亲缘关系提供了重要信息，为今后板栗的高效育种奠定基础。

Abstract:

Chinese chestnut is an important nut tree around the world. Although the types of Chinese chestnut resources are abundant, resource utilization and protection of chestnut accessions are still very limited. Here, we fingerprinted and determined the genetic relationships and core collections of Chinese chestnuts using 18 fluorescently labeled SSR markers generated from 146 chestnut accessions. Our analyses showed that these markers from the tested accessions are highly polymorphic, with an average allele number (N_a) and polymorphic information content (PIC) of 8.100 and 0.622 per locus, respectively. Using these strongly distinguishing markers, we successfully constructed unique fingerprints for 146 chestnut accessions and selected seven of the SSR markers as core markers to rapidly distinguish different accessions. Our exploration of the genetic relationships among the five cultivar groups indicated that Chinese chestnut accessions are divided into three regional type groups: group I (North China (NC) and Northwest China (NWC) cultivar groups), group II (middle and lower reaches of the Yangtze River (MLY) cultivar group) and group III (Southeast China (SEC) and Southwest China (SWC) cultivar groups). Finally, we selected 45 core collection members which represent the most genetic diversity of Chinese chestnut accessions. This study provides valuable information for identifying chestnut accessions and understanding the phylogenetic relationships among cultivar groups, which can serve as the basis for efficient breeding in the future.

Key words: Castanea mollissima , SSR markers , fingerprinting , phylogeny , core collections

. [J]. Journal of Integrative Agriculture, 2021, 20(5): 1277-1286.

NIE Xing-hua, WANG Ze-hua, LIU Ning-wei, SONG Li, YAN Bo-qian, XING Yu, ZHANG Qing, FANG Ke-feng, ZHAO Yong-lian, CHEN Xin, WANG Guang-peng, QIN Ling, CAO Qing-qin. Fingerprinting 146 Chinese chestnut (Castanea mollissima Blume) accessions and selecting a core collection using SSR markers[J]. Journal of Integrative Agriculture, 2021, 20(5): 1277-1286.

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Fingerprinting 146 Chinese chestnut (Castanea mollissima Blume) accessions and selecting a core collection using SSR markers

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