Genome-wide identification and expression profiling of MYB transcription factor genes in radish (Raphanus sativus L.)

doi:10.1016/S2095-3119(20)63308-1

Journal of Integrative Agriculture ›› 2021, Vol. 20 ›› Issue (1): 120-131.DOI: 10.1016/S2095-3119(20)63308-1

所属专题：园艺-分子生物合辑Horticulture — Genetics · Breeding

收稿日期:2020-03-01 出版日期:2021-01-01 发布日期:2020-12-13

Genome-wide identification and expression profiling of MYB transcription factor genes in radish (Raphanus sativus L.)

Everlyne M’mbone MULEKE1*, WANG Yan1*, ZHANG Wan-ting1*, XU Liang1, YING Jia-li1, Bernard K. KARANJA1, ZHU Xian-wen2, FAN Lian-xue1, Zarwali AHMADZAI1, LIU Li-wang1

¹ National Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Horticultural Crop Biology and Genetic Improvement (East China) of Ministry of Agriculture and Rural Affairs, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R.China
² Department of Plant Sciences, North Dakota State University, Fargo, ND 58108, USA

Received:2020-03-01 Online:2021-01-01 Published:2020-12-13
Contact: Correspondence LIU Li-wang, Tel: +86-25-84395563, Fax: +86-25-84395266, E-mail: nauliulw@njau.edu.cn
About author:Everlyne M’mbone MULEKE, E-mail: mmboneve@gmail.com; WANG Yan, E-mail: wangyanhs@njau.edu.cn; ZHANG Wan-ting, E-mail: 2018104061@njau.edu.cn; * These authors contributed equally to this study.
Supported by:
This work was in part supported by the National Key Research and Development Program of China (2017YFD0101806), the Open Funds of State Key Laboratory of Crop Genetics and Germplasm Enhancement, China (ZW201709), the Key Laboratory of Biology and Genetics Improvement of Horticultural Crops, Ministry of Agriculture and Rural Affairs, China (IVF201706) and the Jiangsu Agricultural Science and Technology Innovation Fund, China (CX(19)3045).

摘要/Abstract

摘要：

萝卜是一种重要的十字花科根菜类蔬菜作物，在其有色的根中有高水平的花青素累积。MYB转录因子(TFs)在植物发育和花青素代谢中起着重要作用，并且PAP1/2能促进花青素生物合成基因的表达。本研究在萝卜基因组中共鉴定出187个RsMYB基因，并将其分为32个亚家族；其中159个RsMYB基因被定位在9条染色体上。在4个不同颜色的萝卜品种肉质根发育阶段，14个RsMYB基因表现出差异的表达模式。一些RsMYB基因在成熟期有色根组织中高表达，这些基因包括RsMYB41，RsMYB117以及与PAP1/2同源的RsMYB132在‘NAU-YH’的红色根皮中高表达，RsMYB65和RsMYB159基因在‘NAU-YZH’的紫色根皮中高表达，表明这些RsMYB基因可能促进萝卜肉质根花青素积累。研究结果为进一步研究萝卜RsMYB基因功能特性提供有价值的信息，并有助于阐明萝卜花青素生物合成的分子机制。

Abstract:

Radish (Raphanus sativus L.), an important root vegetable crop of the Brassicaceae family, has a high level of anthocyanin accumulation in its pigment root tissues. It was reported that MYB transcription factors (TFs) play vital roles in plant development and anthocyanin metabolism, and the PAP1/2 could promote expression of anthocyanin biosynthesis genes. In this study, a total of 187 radish MYB genes (RsMYBs) were identified in the radish genome and clustered into 32 subfamilies. Among them, 159 RsMYBs were localized on nine radish chromosomes. Interestingly, 14 RsMYBs exhibited differential expression profiles in different taproot developmental stages among four differently colored radish lines. A number of RsMYBs were highly expressed in the pigmented root tissues at the maturity stage, several genes including RsMYB41, RsMYB117, and RsMYB132 being homologous to PAP1/2, showed high expression levels in the red skin of NAU-YH (red skin-white flesh) taproot, while RsMYB65 and RsMYB159 were highly expressed in the purple root skin of NAU-YZH (purple skin-red flesh), indicating that these RsMYBs might positively regulate the process of anthocyanin accumulation in radish taproot. These results would provide valuable information for further functional characterization of RsMYBs, and facilitate clarifying the molecular mechanism underlying anthocyanin biosynthesis in radish.

Key words: radish , MYB transcription factors , anthocyanin biosynthesis , expression profiling

. [J]. Journal of Integrative Agriculture, 2021, 20(1): 120-131.

Everlyne M’mbone MULEKE, WANG Yan, ZHANG Wan-ting, XU Liang, YING Jia-li, Bernard K. KARANJA, ZHU Xian-wen, FAN Lian-xue, Zarwali AHMADZAI, LIU Li-wang. Genome-wide identification and expression profiling of MYB transcription factor genes in radish (Raphanus sativus L.)[J]. Journal of Integrative Agriculture, 2021, 20(1): 120-131.

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Genome-wide identification and expression profiling of MYB transcription factor genes in radish (Raphanus sativus L.)

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