Decreasing detection frequency of MITE (MCLas-A) in the population of ‘Candidatus Liberibacter asiaticus’ recently collected in southern China

doi:10.1016/S2095-3119(20)63217-8

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (10): 2597-2601.DOI: 10.1016/S2095-3119(20)63217-8

所属专题：植物病理合辑Plant Protection—Plant Pathology；植物病毒合辑Plant Virus

• 论文 • 上一篇

收稿日期:2019-12-30 出版日期:2020-10-01 发布日期:2020-09-08

Decreasing detection frequency of MITE (MCLas-A) in the population of ‘Candidatus Liberibacter asiaticus’ recently collected in southern China

CUI Xue-jin, ZENG Chun-hua, LIU Ke-hong, TENG Cai-ling, ZHOU Chang-yong, WANG Xue-feng

National Citrus Engineering Research Center, Citrus Research Institute, Chinese Academy of Agricultural Sciences/Southwest University, Chongqing 400712, P.R.China

Received:2019-12-30 Online:2020-10-01 Published:2020-09-08
Contact: Correspondence WANG Xue-feng, Tel: +86-23-68349338, E-mail: wangxuefeng@cric.cn
About author:CUI Xue-jin, E-mail: cuixuejin@163.com;
Supported by:
This study was funded by the National Natural Science Foundation of China (31671992, 31871925), the National Key R&D Program of China (2018YFD0201500), and the Key R&D Program of Guangxi, China (Gui Ke AA18118046).

摘要/Abstract

Abstract:

An active miniature inverted-repeat transposable element (MITE), MCLas-A, was previously identified from ‘Candidatus Liberibacter asiaticus’ known to be associated with citrus Huanglongbing (HLB, yellow shoot disease). To explore the recent transposition status of MCLas-A, 389 ‘Ca. L. asiaticus’ strains collected from nine regions in China were amplified using a specific primer set and three representative ‘Ca. L. asiaticus’ strains were analyzed by next-generation sequencing (NGS) approach. PCR and genomic analysis showed that the entire MCLas-A was only present in 1.80% (7/389) and the jumping-out type of the MITE was predominant (81.23%) in samples tested, suggesting high frequency transposition occurred in ‘Ca. L. asiaticus’ strains recently collected from China. Biological roles of transposition of the active MITE remain to be determined.

Key words: Huanglongbing , ‘Candidatus Liberibacter asiaticus&rsquo, miniature inverted-repeat transposable element , MITE

. [J]. Journal of Integrative Agriculture, 2020, 19(10): 2597-2601.

CUI Xue-jin, ZENG Chun-hua, LIU Ke-hong, TENG Cai-ling, ZHOU Chang-yong, WANG Xue-feng. Decreasing detection frequency of MITE (MCLas-A) in the population of ‘Candidatus Liberibacter asiaticus’ recently collected in southern China[J]. Journal of Integrative Agriculture, 2020, 19(10): 2597-2601.

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Decreasing detection frequency of MITE (MCLas-A) in the population of ‘Candidatus Liberibacter asiaticus’ recently collected in southern China

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