Development of peanut varieties with high oil content by in vitro mutagenesis and screening

doi:10.1016/S2095-3119(20)63182-3

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (12): 2974-2982.DOI: 10.1016/S2095-3119(20)63182-3

收稿日期:2019-09-09 出版日期:2020-12-01 发布日期:2020-11-19

Development of peanut varieties with high oil content by in vitro mutagenesis and screening

WANG Jing-shan^1*, SHI Lei^2*, LIU Yue^3*, ZHAO Ming-xia¹, WANG Xia¹, QIAO Li-xian¹, SUI Jiong-ming¹, LI Guan¹, ZHU Hong¹, YU Shan-lin¹

¹ College of Agronomy, Qingdao Agricultural University/Shandong Provincial Key Laboratory of Dryland Farming Technology, Qingdao 266109, P.R.China
² Haidu College, Qingdao Agricultural University, Laiyang 265200, P.R.China
³ College of Horticulture, Qingdao Agricultural University, Qingdao 266109, P.R.China

Received:2019-09-09 Online:2020-12-01 Published:2020-11-19
Contact: Correspondence YU Shan-lin, E-mail: yshanlin1956@163.com
About author:WANG Jing-shan, E-mail: jswang319@126.com; * These authors contributed equally to this study.
Supported by:
This research was funded by the National Natural Science Foundation of China (31571705 and 31872875), the Key R&D Program of Shandong Province, China (2018GNC111014) and the Open Oroject of the Key Lab of Oil Crop Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs of China (KF2018008).

摘要/Abstract

摘要： 花生是重要的油料作物，培育高油品种是花生育种的主要目标之一。研究表明，干旱胁迫下花生叶片渗透压与其籽仁含油量呈显著负相关。基于这一原理，我们创建了利用离体诱变和渗透压定向筛选培育高油花生新品种的有效方法。以花生品种花育20号的种子胚小叶为外植体，平阳霉素(PYM)为诱变剂，羟脯氨酸(HYP)为渗透压调节剂进行离体诱变和定向筛选，最终获得15株HYP耐性再生植株。每个再生植株均有含油量 >55%的后代（而花育20号含油量为49.5%）。从HYP耐性植株后代中培育出3个高产高油花生新品种，宇花4号、宇花9号、宇花14号，含油量分别为57.7%、61.1%、59.3%。结果表明，PYM离体诱变后进行HYP离体定向筛选，是培育高油花生品种的有效途径。

Abstract:

Peanut (Arachis hypogaea L.) is an important oil crop globally and high oil content is one of the major targets in peanut breeding programs. Previous studies indicated that the osmotic pressure (OP) of the leaves of peanut plants subjected to drought stress was negatively correlated with kernel oil content. Based on this knowledge, we established a practical and reliable method for creating new peanut varieties with high oil content using in vitro mutagenesis and directional OP-based selection. Using embryonic leaflets of peanut variety Huayu 20 as explants, pingyangmycin (PYM) as the mutagen, and hydroxyproline (HYP) as the OP regulator, we developed 15 HYP-tolerant regenerated plants. For each regenerated plant, we selected offspring with oil content>55% (relative to 49.5% for Huayu 20). We developed and released three new peanut varieties with high yield and high oil content from the offspring of the HYP-tolerant regenerated plants. The three new varieties were named as Yuhua 4, Yuhua 9 and Yuhua 14 and their oil contents were 57.7, 61.1 and 59.3%, respectively. The results indicate that in vitro mutagenesis with PYM followed by directed screening with HYP is a useful approach for breeding peanut varieties with high oil contents.

Key words: Arachis hypogaea , in vitro mutagenesis , HYP-directed selection , high oil variety

. [J]. Journal of Integrative Agriculture, 2020, 19(12): 2974-2982.

WANG Jing-shan, SHI Lei, LIU Yue, ZHAO Ming-xia, WANG Xia, QIAO Li-xian, SUI Jiong-ming, LI Guan, ZHU Hong, YU Shan-lin. Development of peanut varieties with high oil content by in vitro mutagenesis and screening[J]. Journal of Integrative Agriculture, 2020, 19(12): 2974-2982.

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Development of peanut varieties with high oil content by in vitro mutagenesis and screening

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