Genome-wide identification and expression analysis of anthocyanin biosynthetic genes in Brassica juncea

doi:10.1016/S2095-3119(20)63172-0

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (5): 1250-1260.DOI: 10.1016/S2095-3119(20)63172-0

所属专题：园艺-分子生物合辑Horticulture — Genetics · Breeding

收稿日期:2019-07-12 出版日期:2020-04-01 发布日期:2020-03-25

Genome-wide identification and expression analysis of anthocyanin biosynthetic genes in Brassica juncea

ZHANG Da-wei^{1, 2}, LIU Li-li^{1, 2}, ZHOU Ding-^{gang1, 2}, LIU Xian-jun³, LIU Zhong-song⁴, YAN Ming-li^{1, 2}

¹ School of Life Science, Hunan University of Science and Technology, Xiangtan 411201, P.R.China
² Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, Xiangtan 411201, P.R.China
³ College of Life Sciences, Resources and Environment Sciences, Yichun University, Yichun 333600, P.R.China
⁴ Oilseed Research Institute, Hunan Agricultural University, Changsha 410128, P.R.China

Received:2019-07-12 Online:2020-04-01 Published:2020-03-25
Contact: Correspondence YAN Ming-li, Tel/Fax: +86-731-58291416, E-mail: ymljack@126.com
About author: ZHANG Da-wei, E-mail: zhangdawei.hnust@foxmail.com;
Supported by:
This work was funded by the National Key Research and Development Program of China (2016YFD0100202), the Natural Science Foundation of Hunan Province, China (2016JJ1010) and the Scientific Research Fund of Hunan Provincial Education Department, China (18C0305, 17K035, 17C0652, and 17C0653).

摘要/Abstract

Abstract:

Anthocyanins confer the wide range of colors for plants and also play beneficial health roles as potentially protective factors against heart disease and cancer. Brassica juncea is cultivated as an edible oil resource and vegetable crop worldwide, thus elucidating the anthocyanin biosynthetic pathway would be helpful to improve the nutritional quality of Brassica juncea through the breeding and cultivating of high anthocyanin content varieties. Herein, 129 genes in B. juncea were identified as orthologs of 41 anthocyanin biosynthetic genes (ABGs) in Arabidopsis thaliana by comparative genomic analyses. The B. juncea ABGs have expanded by whole genome triplication and subsequent allopolyploidizatoin, but lost mainly during the whole genome triplication between B. rapa/B. nigra and A. thaliana, rather than the allopolyploidization process between B. juncea and B. rapa/B. nigra, leading to different copy numbers retention of A. thaliana homologous genes. Although the overall expansion levels ABGs were similar to the whole genome, more negative regulatory genes were retained in the anthocyanin biosynthesis regulatory system. Transcriptional analysis of B. juncea with different anthocyanin accumulation showed that BjDFR, BjTT19, BjTT8 are significantly up-regulated in plants with purple leaves as compared with green leaves. The overexpression of BjTT8 and these target genes which were involved in late anthocyanin biosynthesis and transport might account for increasing levels of anthocyanin accumulation in purple leaves. Our results could promote the understanding of the genetic mechanism of anthocyanin biosynthesis in B. juncea.

Key words: anthocyanin biosynthetic genes , B. juncea , comparative genomic analysis , transcriptome , leaf color

. [J]. Journal of Integrative Agriculture, 2020, 19(5): 1250-1260.

ZHANG Da-wei, LIU Li-li, ZHOU Ding-gang, LIU Xian-jun, LIU Zhong-song, YAN Ming-li.

Genome-wide identification and expression analysis of anthocyanin biosynthetic genes in Brassica juncea

[J]. Journal of Integrative Agriculture, 2020, 19(5): 1250-1260.

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Genome-wide identification and expression analysis of anthocyanin biosynthetic genes in Brassica juncea

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