Susceptibility and tissue specificity of Spodoptera frugiperda to Junonia coenia densovirus

doi:10.1016/S2095-3119(20)63163-X

摘要/Abstract

摘要：

草地贪夜蛾又名秋行军虫，严重危害玉米和水稻等农作物，目前已经入侵我国。昆虫病毒作为生物农药，在控制害虫过程中具有重要的作用。昆虫浓核病毒具有环境友好和高效杀虫等优点，是一种潜在的生物杀虫剂。我们采用多种昆虫细胞系和鳞翅目昆虫幼虫从鹿眼蛱蝶浓核病毒全基因组感染性克隆成功拯救了病毒粒子。采用感染性克隆质粒转染的昆虫细胞系的匀浆物饲喂二龄草地贪夜蛾幼虫，具有致死效应。该浓核病毒感染致死的斜纹夜蛾的匀浆物对二龄草地贪夜蛾的半致死剂量是1.76×10⁸ 病毒基因组拷贝，高于斜纹夜蛾（7.39×10⁷ 病毒基因组拷贝）和棉铃虫（9.71×10⁷ 病毒基因组拷贝）。这种匀浆物对草地贪夜蛾的半致死时间是6.96 d，高于斜纹夜蛾（6.18）和棉铃虫（5.94 d）。该病毒能够感染棉铃虫的脂肪体，但是不能感染草地贪夜蛾和斜纹夜蛾的脂肪体。虽然这三种昆虫都对该病毒敏感，但是毒力大小具有差异。鹿眼蛱蝶浓核病毒可以作为控制草地贪夜蛾的潜在的生物杀虫剂。

Abstract:

The fall armyworm, Spodoptera frugiperda, which destroys many economic crops such as rice and maize, has recently invaded China. Insect viruses as biological control agents play important roles in killing pests. One potential viral insecticide is the environmentally highly infective and virulent densovirus. We successfully rescued Junonia coenia densovirus (JcDV) using its infectious clone in different insect cell lines and larvae of three insect species. Results showed that the lysate of cultured insect cells transfected by the JcDV infectious clone killed the 2nd instar S. frugiperda. The LD₅₀ of homogenate from JcDV-infected Spodoptera litura to the 2nd instar S. frugiperda (1.76×10⁸ viral genome copies per larva during 10 d post infection) was higher than that of the 2nd instar S. litura (7.39×10⁷ JcDV genome copies) or Helicoverpa armigera larvae (9.71×10⁷ JcDV genome copies). The LT₅₀ of the S. litura homogenate (2.60×10⁹ viral genome copies each larva) to the 2nd instar S. frugiperda was 6.96 d, longer than that of the S. litura (6.18 d) or the 2nd instar H. armigera (5.94 d). JcDV could infect the fat body of H. armigera, but not S. frugiperda or S. litura. Although JcDV can infect all three lepidopteran species, their susceptibility to the virus differs. JcDV has great potential as a biological control agent against pests such as S. frugiperda.

Key words: Spodoptera frugiperda , Junonia coenia densovirus , rescue of virus , susceptibility , biological control

. [J]. Journal of Integrative Agriculture, 2021, 20(3): 840-849.

CHEN Zu-wen, YANG Yan-chao, ZHANG Jian-feng, JIN Ming-hui, XIAO Yu-tao, XIA Zhi-chao, LIU Yuan-yuan, YU Sai-zhen, YANG Yong-bo, WANG Yuan, LI Yi, LIU Kai-yu. Susceptibility and tissue specificity of Spodoptera frugiperda to Junonia coenia densovirus[J]. Journal of Integrative Agriculture, 2021, 20(3): 840-849.

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