Identification and characterization of a TLR13 gene homologue from Laodelphax striatellus involved in the immune response induced by rice stripe virus

doi:10.1016/S2095-3119(19)62795-4

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (1): 183-192.DOI: 10.1016/S2095-3119(19)62795-4

所属专题：昆虫合辑Plant Protection—Entomolgy；昆虫分子生物学合辑Insect Molecular Biology；昆虫和植物互作合辑Insect and Plant Interact

收稿日期:2019-03-26 出版日期:2020-01-01 发布日期:2019-12-23

Identification and characterization of a TLR13 gene homologue from Laodelphax striatellus involved in the immune response induced by rice stripe virus

ZHOU Xue^1*, HU Jia^2*, FU Mei-li³, JIN Ping³, ZHANG Yun-ye², XIANG Ying², LI Yao², MA Fei³

¹ School of Chemistry and Biological Engineering, Nanjing Normal University Taizhou College, Taizhou 225300, P.R.China
² College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, P.R.China
³ Laboratory for Comparative Genomics and Bioinformatics & Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210046, P.R.China

Received:2019-03-26 Online:2020-01-01 Published:2019-12-23
Contact: Correspondence MA Fei, E-mail: mafei01@tsinghua.org.cn; LI Yao, E-mail: liyao@yzu.edu.cn
About author: * These authors contributed equally to this study.
Supported by:
This work was supported by grants from the National Natural Science Foundation of China (31572324), the General Program of Natural Science Foundation of the Higher Education Institutions of Jiangsu Province, China (16KJB180017), the College Students’ Innovation and Entrepreneurship Training Program Project of Jiangsu Province, China (201813843009Y), the Jiangsu Agricultural Scientific Self-innovation Fund, China (CX[15]1053) and a grant from Priority Academic Program Development of Jiangsu Higher Education Institutions, China.

摘要/Abstract

Abstract:

Toll-like receptors (TLRs) are the critical superfamily homologues that initiate sensing of the invasion of pathogens by the Toll pathway. As one of several intracellular nucleic acid-sensing TLRs, TLR13 is activated by an unmethylated motif present in the large ribosomal subunit of bacterial RNA. However, little attention has been paid to the function of TLR13 gene homologue from Laodelphax striatellus (designated as LsToll-13) in the immune response to rice stripe virus (RSV). Herein, LsToll-13 was cloned and characterized using RACE-PCR. Phylogenetic analysis showed that LsToll-13 was clustered with the TLR13 from six insects. Real-time PCR analysis demonstrated that the expression level of LsToll-13 was significantly reduced in L.?striatellus with RSV infection compared with that in the naive strain. When the expression of LsToll-13 was significantly up-regulated at 6 h after bacterial infection, the expression of ribonucleoprotein (RNP) indicated that the RSV titer in the host insect was significantly suppressed. Upon knockdown of LsToll-13, using RNA interference (RNAi) in L.?striatellus, the expression level of RNP was significantly increased with enhanced RSV accumulation, suggesting that LsToll-13 potentially protects L.?striatellus from RSV infection. Taken together, our results indicated that LsToll-13 might be involved in the immune response of L.?striatellus to RSV infection, and provided a new insight into further elucidating the molecular mechanisms of complex pathogen-host interactions and integrative pest management.

Key words: Laodelphax striatellus , RSV , TLR13 , RNAi , antiviral immunity

. [J]. Journal of Integrative Agriculture, 2020, 19(1): 183-192.

ZHOU Xue, HU Jia, FU Mei-li, JIN Ping, ZHANG Yun-ye, XIANG Ying, LI Yao, MA Fei . Identification and characterization of a TLR13 gene homologue from Laodelphax striatellus involved in the immune response induced by rice stripe virus[J]. Journal of Integrative Agriculture, 2020, 19(1): 183-192.

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Identification and characterization of a TLR13 gene homologue from Laodelphax striatellus involved in the immune response induced by rice stripe virus

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