Allelochemical p-hydroxybenzoic acid inhibits root growth via regulating ROS accumulation in cucumber (Cucumis sativus L.)

doi:10.1016/S2095-3119(19)62781-4

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (2): 518-527.DOI: 10.1016/S2095-3119(19)62781-4

所属专题：园艺-栽培生理/资源品质合辑Horticulture — Physiology · Biochemistry · Cultivation

收稿日期:2019-02-28 出版日期:2020-02-01 发布日期:2020-01-18

Allelochemical p-hydroxybenzoic acid inhibits root growth via regulating ROS accumulation in cucumber (Cucumis sativus L.)

HUANG Cheng-zhen¹, XU Lei¹, Sun Jin-jing², ZHANG Zhong-hu^a2, FU Mei-lan³, TENG Hui-yin^g3, YI Ke-ke¹

¹ Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture and Rural Affairs/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 10081, P.R.China
² Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 10081, P.R.China
3 The Semi-arid Agriculture Engineering & Technology Research Center of China, Hebei 050051, P.R.China

Received:2019-02-28 Online:2020-02-01 Published:2020-01-18
Contact: Correspondence XU Lei, Tel: +86-10-82108643, E-mail: xuleisy@zju.edu.cn
Supported by:
This research was supported by the National Key Research and Development Program of China (2018YFD1000803) and the National Natural Science Foundation of China (31800246).

摘要/Abstract

Abstract:

Allelopathy is prevalent in agricultural ecosystems and mediated by plant-derived secondary metabolites (allelochemicals). Allelochemicals are released by donor plants and affect the root growth and development of receptor plants. Allelopathy is responsible for the continuous cropping obstacles in cucumber (Cucumis sativus L.). p-Hydroxybenzoic acid (pHBA), an autotoxin from root exudates of cucumber, has been proposed to be an important allelopathic chemical. However, the molecular mechanism by which pHBA affect root growth and development in cucumber is unknown. Here, we found that pHBA treatment suppressed root growth of cucumber by reducing the meristem activity and cell length. This root growth defect is caused by reduced reactive oxygen species (ROS) accumulation in root tips. After pHBA treatment, the expression levels of several ROS-scavenging-related genes were increased, including peroxidase (POD), catalase (CAT) and metallothionein (MT). Moreover, exogenously application of salicylhydroxamate (SHAM), a peroxidase inhibitor, can partially restore the pHBA treatment induced root growth inhibition. Furthermore, we found that there is natural variation for the inhibitory effect of pHBA on root growth. We also showed that pHBA treatment could maintain higher level of ROS accumulated in the pHBA less sensitive cucumber than that in the pHBA-sensitive cucumber. These results suggest that pHBA inhibits root growth by reducing root tip ROS level in cucumber.

Key words: allelopathy , cucumber , reactive oxygen species , natural variation

. [J]. Journal of Integrative Agriculture, 2020, 19(2): 518-527.

HUANG Cheng-zhen, XU Lei, Sun Jin-jing, ZHANG Zhong-hua, FU Mei-lan, TENG Hui-ying, YI Ke-ke.

Allelochemical p-hydroxybenzoic acid inhibits root growth via regulating ROS accumulation in cucumber (Cucumis sativus L.)

[J]. Journal of Integrative Agriculture, 2020, 19(2): 518-527.

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Allelochemical p-hydroxybenzoic acid inhibits root growth via regulating ROS accumulation in cucumber (Cucumis sativus L.)

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