Molecular characterization, expression and function analysis of eukaryotic translation initiation factor (eIF1A) in Mangifera indica

doi:10.1016/S2095-3119(19)62774-7

Journal of Integrative Agriculture ›› 2019, Vol. 18 ›› Issue (11): 2505-2513.DOI: 10.1016/S2095-3119(19)62774-7

收稿日期:2018-12-05 出版日期:2019-11-01 发布日期:2019-11-02

Molecular characterization, expression and function analysis of eukaryotic translation initiation factor (eIF1A) in Mangifera indica

LI Li-shu^{1, 2*}, LUO Cong^1*, AN Zhen-yu³, LIU Zhao-liang¹, DONG Long³, YU Hai-xia¹, HE Xin-hua¹

¹ The State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/College of Agriculture, Guangxi University, Nanning 530004, P.R.China
² Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, P.R.China
³ Horticulture Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, P.R.China

Received:2018-12-05 Online:2019-11-01 Published:2019-11-02
Contact: Correspondence HE Xin-hua, E-mail: honest66222@163.com
About author: LI Li-shu, E-mail: shukitty@126.com; * These authors contributed equally to this study.
Supported by:
This research was supported by the National Natural Science Foundation of China (31660561), the Natural Science Foundation of Guangxi, China (2015GXNSFAA139052), the Key Research and Development Project of Guangxi, China (GXKJ-AB17292010), and the Major Science and Technology Projects of Guangxi, China (GXKJ-AA17204097-3 and GXKJ-AA172040 26-2), the Innovation Team of Guangxi Mango Industry Project, China (nycytxgxcxtd-06-02), and the State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, China (SKLCUSA-a201906).

摘要/Abstract

Abstract:

Eukaryotic translation initiation factor 1A (eIF1A) functions as an important regulatory factor of protein synthesis and plays a crucial role in responses to abiotic stresses in plants. However, little is known about the eIF1A gene involved in fruit development and stress response of mango. In this study, the MieIF1A-b gene was isolated from Mangifera indica, and contains a 435-bp open reading frame, which encodes a putative protein of 144 amino acids (GenBank accession number: KP676599). The predicted MieIF1A-b protein had a molecular weight of 16.39 kDa with a pI of 4.6. Sequence homology analysis showed that MieIF1A-b shared high homology with Elaeis guineensis, Manihot esculenta, and Populus trichocarpa, with 96 and 95% identity, respectively. Quantitative reverse transcriptative PCR (qRT-PCR) analyses indicated that MieIF1A-b was expressed in all tested tissues, and had the highest expression level in fruit 80 d after flowering. The expression of MieIF1A-b was obviously regulated by NaCl and H₂O₂ treatments in leaves. Functional analysis indicated that the overexpression of MieIF1A-b in transgenic Arabidopsis thaliana enhanced the growth, phenotype and salinity tolerance compared with wild-type (WT) plants. The results indicated that MieIF1A-b may be correlated with the control of fruit development and salt adaptation, and it was a candidate gene for abiotic stress in mango.

Key words: Mangifera indica , MieIF1A-b gene , gene clone , expression , functional analysis

. [J]. Journal of Integrative Agriculture, 2019, 18(11): 2505-2513.

LI Li-shu, LUO Cong, AN Zhen-yu, LIU Zhao-liang, DONG Long, YU Hai-xia, HE Xin-hua . Molecular characterization, expression and function analysis of eukaryotic translation initiation factor (eIF1A) in Mangifera indica[J]. Journal of Integrative Agriculture, 2019, 18(11): 2505-2513.

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Molecular characterization, expression and function analysis of eukaryotic translation initiation factor (eIF1A) in Mangifera indica

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