Identification and developmental expression of putative gene encoding juvenile hormone esterase (CpJHE-like) in codling moth, Cydia pomonella (L.)

doi:10.1016/S2095-3119(19)62682-1

Journal of Integrative Agriculture ›› 2019, Vol. 18 ›› Issue (7): 1624-1633.DOI: 10.1016/S2095-3119(19)62682-1

收稿日期:2019-01-31 修回日期:2019-03-18 出版日期:2019-07-01 发布日期:2019-07-01

Identification and developmental expression of putative gene encoding juvenile hormone esterase (CpJHE-like) in codling moth, Cydia pomonella (L.)

HUANG Cong^{1, 3}, WU Qiang², JIANG Chun-yan^{2, 4}, XING Long-sheng³, SHI Guo-liang^{2, 4}, ZHANG Bin^{3, 4}, QIAN Wan-qiang³, LI You-zhi¹, XI Yu³, YANG Nian-wan², WAN Fang-hao^{1, 2, 3}

¹ College of Plant Protection, Hunan Agricultural University, Changsha 410128, P.R.China
² State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
³ Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, P.R.China
⁴ College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, P.R.China

Received:2019-01-31 Revised:2019-03-18 Online:2019-07-01 Published:2019-07-01
Contact: Correspondence WAN Fang-hao, Tel/Fax: +86-10-82109572, E-mail: wanfanghao@caas.cn; YANG Nian-wan, Tel/Fax: +86-10-82109572, E-mail: yangnianwan@caas.cn
About author:HUANG Cong, Mobile: +86-18040634335, E-mail: huangcong16@163.com;
Supported by:
This work was supported by the National Key Research and Development Program of China (2016YFC1201200, 2017YFC1200600 and 2016YFC1200602), the Basic Research on Science and Technology Project of Shenzhen, China (JCYJ20160530191934833), and the Dapeng New District Industrial Development Special Fund of Shenzhen, China (KY20180215).

摘要/Abstract

Abstract:

Juvenile hormone esterase (JHE) is a key enzyme for insects, playing an important role in the regulation of insect growth, development, diapause and reproduction. We identified a complete putative JHE of Cydia pomonella (CpJHE-like) which is comprised of a 1 761 bp coding sequence (CDS) encoding 587 amino acid residues from the transcriptome data. The deduced protein sequence of CpJHE-like showed the highest identity of 60.44% with the Adoxophyes honmai JHE (AhJHE) and the minimal identity of 25.81% with Aedes aegypti JHE (AaJHE). CpJHE-like exhibited all the seven typical motifs of the functional JHEs and had the highly consistent tertiary structure with Manduca sexta JHE (MsJHE). Phylogenetic analysis showed that the CpJHE-like was close to two JHEs from the family Tortricidae. The CpJHE-like transcript level take a leap in the 3-day-old fifth instar larva, increased about 300-fold compared to the basal level. Tissue-specific expression profile showed that the CpJHE-like transcript was expressed mainly in the fat body. This study indicates that the CpJHE-like is the functional JHE, which may play vital roles in the development and reproduction of C. pomonella.

Key words: juvenile hormone esterase , CpJHE-like , codling moth , Cydia pomonella

. [J]. Journal of Integrative Agriculture, 2019, 18(7): 1624-1633.

HUANG Cong, WU Qiang, JIANG Chun-yan, XING Long-sheng, SHI Guo-liang, ZHANG Bin, QIAN Wan-qiang, LI You-zhi, XI Yu, YANG Nian-wan, WAN Fang-hao. Identification and developmental expression of putative gene encoding juvenile hormone esterase (CpJHE-like) in codling moth, Cydia pomonella (L.)[J]. Journal of Integrative Agriculture, 2019, 18(7): 1624-1633.

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Identification and developmental expression of putative gene encoding juvenile hormone esterase (CpJHE-like) in codling moth, Cydia pomonella (L.)

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