Journal of Integrative Agriculture ›› 2019, Vol. 18 ›› Issue (6): 1222-1229.DOI: 10.1016/S2095-3119(19)62674-2

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  • 收稿日期:2018-12-20 出版日期:2019-06-01 发布日期:2019-05-29

Genetic dissection of hexanol content in soybean seed through genome-wide association analysis

XIA Ning1, 2, YAN Wen-bing1, WANG Xiao-qi1, SHAO Yu-peng1, YANG Ming-ming1, WANG Zhi-kun1, ZHAN Yu-hang1, TENG Wei-li1, HAN Ying-peng1, SHI Yan-guo2   

  1. 1 Key Laboratory of Soybean Biology, Ministry of Education/Key Laboratory of Soybean Biology and Breeding/Genetics, Ministry of Agriculture and Rural Affairs/Agricultural College, Northeast Agricultural University, Harbin 150030, P.R.China
    2 School of Food Engineering, Harbin University of Commerce, Harbin 150030, P.R.China
  • Received:2018-12-20 Online:2019-06-01 Published:2019-05-29
  • Contact: Correspondence HAN Ying-peng, Tel: +86-451-55191043, Fax: +86-451-55103336, E-mail: 347437583@qq.com; SHI Yan-guo E-mail: HRBshiyanguo@126.com
  • Supported by:
    This study was financially supported by the National R&D Program of China (2017YFD0101302), the National Natural Science Foundation of China (31671717 and 31471517), the National Ten-thousand Talents Program, Heilongjiang Provincial Project, China (GX17B002, JC2018007 and C2018016), the Postdoctoral Fund in Heilongjiang Province, China (LBH-Z15017 and LBH-Q17015), the ‘Youth Innovation Talent’ Project of the general undergraduate universities in Heilongjiang Province, China (UNPYSCT-2016145), and the ‘Academic Backbone’ Project of Northeast Agricultural University, China (17XG22).

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Abstract: Hexanol is a major compound contributing to the off-flavors (the bean-like odor) of soybean derived soymilk. The most effective way to reduce the off-flavors of soymilk is the screening and utilization of soybean cultivars with improved hexanol content. However, no genome-wide genetic analysis for this particular trait has been conducted to date. The objective of the present study was to dissect the genetic basis of hexanol content in soybean seed through genome-wide association analysis (GWAS). A total of 105 soybean accessions were analyzed for hexanol content in a three-year experiments and genotyped by sequencing using the specific locus amplified fragment sequencing (SLAF-seq) approach. A total of 25 724 single nucleotide polymorphisms (SNPs) were obtained with minor allele frequencies (MAF)>5%. GWAS showed that 25 quantitative trait nucleotides (QTNs) were significantly associated with the hexanol concentration in soybean seed. These identified QTNs distributed on different genomic regions of the 15 chromosomes. A total of 91 genes were predicted as candidate genes underlying the seed hexanol level and six candidates were predicted possibly underlying the seed hexanol by gene based association. In this study, GWAS has been proven to be an effective way to dissect the genetic basis of the hexanol concentration in multiple genetic backgrounds. The identified beneficial alleles and candidate genes might be valuable for the improvement of marker-assisted breeding efficiency for low hexanol level and help to explore possible molecular mechanisms underlying hexanol content in soybean seed.

Key words: genome-wide association mapping ,  soybean ,  hexanol concentration ,  candidate genes