Serologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China

doi:10.1016/S2095-3119(19)62650-X

Journal of Integrative Agriculture ›› 2019, Vol. 18 ›› Issue (9): 2133-2140.DOI: 10.1016/S2095-3119(19)62650-X

收稿日期:2018-08-13 出版日期:2019-09-01 发布日期:2019-08-31

Serologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China

GUO Huan-cheng^1*, REN Zhao-wen^1*, DING Mei-ming², XIAO Wan-jun³, PENG Peng⁴, HE Biao¹, FENG Ye¹, LIU Yan¹, LI Xing-yu¹, CAI Jian-qiu¹, ZHANG Bi-kai¹, LUO Qing-hua¹, TU Chang-chun^{1, 5}

¹ Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun 130122, P.R.China
² Division of Wildlife Conservation and Nature Reserve Management, Greater Xing’an Mountains Forestry Group Corporation, Jiagedaqi 165000, P.R.China
³ Division of Wildlife Conservation and Nature Reserve Management, Jilin Forestry Administration, Changchun 130022, P.R.China
⁴ State Forestry Administration, Shenyang 110034, P.R.China
⁵ Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonose, Yangzhou University, Yangzhou 225009, P.R.China

Received:2018-08-13 Online:2019-09-01 Published:2019-08-31
Contact: Correspondence TU Chang-chun, E-mail: changchun_tu@hotmail.com
About author:GUO Huan-cheng, E-mail: guohc2013@126.com; REN Zhao-wen, E-mail: 670989008@qq.com; * These authors contributed equally to this study.
Supported by:
This work was supported by the National Key Research and Development Program of China (2016YFC1201600), and Wildlife Borne Infectious Diseases Monitoring Project of the State Forestry Administration (SFA) of China.

摘要/Abstract

Abstract:

Hybrid wild boar husbandry is an important component of livestock production in Northeast China. However, the current disease situation of these animals is largely unknown due to a lack of disease surveillance. The present study was conducted to determine the prevalence of several important viral diseases in the hybrid wild boar population of Northeast China. Between September 2015 to December 2016, 169 blood and 61 tissue samples were collected from apparently healthy hybrid wild boars from farms in Jilin, Inner Mongolia and Heilongjiang provinces. ELISA detected serum antibodies against classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), porcine circovirus type 2 (PCV2) and Japanese encephalitis virus (JEV), but not against African swine fever virus (ASFV), with PCV2 having the highest seropositive rate (87.2–100% in different farms). RT-PCR or PCR performed on the processed samples detected only PCV2, with 33.1% (56/169) of blood samples and 32.8% (20/61) of spleen samples being positive, respectively, indicating widespread PCV2 infection in hybrid wild boars. Phylogenetic analysis of 15 PCV2 ORF2 sequences showed that they belong to genotypes PCV2a, PCV2b and PCV2d, with nucleotide and deduced amino acid homologies of 88.5–100% and 88.1–100%, respectively.

Key words: disease transmission , hybrid wild boars , Northeast China , serological and molecular survey , viral diseases

. [J]. Journal of Integrative Agriculture, 2019, 18(9): 2133-2140.

GUO Huan-cheng, REN Zhao-wen, DING Mei-ming, XIAO Wan-jun, PENG Peng, HE Biao, FENG Ye, LIU Yan, LI Xing-yu, CAI Jian-qiu, ZHANG Bi-kai, LUO Qing-hua, TU Chang-chun . Serologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China[J]. Journal of Integrative Agriculture, 2019, 18(9): 2133-2140.

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Serologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China

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