Transcriptomic analyses reveal new genes and networks response to H5N1 influenza viruses in duck (Anas platyrhynchos)

doi:10.1016/S2095-3119(19)62646-8

Journal of Integrative Agriculture ›› 2019, Vol. 18 ›› Issue (7): 1460-1472.DOI: 10.1016/S2095-3119(19)62646-8

收稿日期:2019-01-22 修回日期:2019-01-31 出版日期:2019-07-01 发布日期:2019-07-01

Transcriptomic analyses reveal new genes and networks response to H5N1 influenza viruses in duck (Anas platyrhynchos)

HUANG Yin-hua^1*, FENG Hua-peng^2*, HUANG Li-ren^2*, YI Kang³, RONG En-guang¹, CHEN Xiao-yun⁴, LI Jian-wen³, WANG Zeng², ZHU Peng-yang², LIU Xiao-juan¹, WANG Xiao-xue¹, HU Jia-xiang¹, LIU Xin³, CHEN Hua-lan², WANG Jun...p>3, LI Ning¹

¹ State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.China
² State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.China
³ BGI-Shenzhen, Shenzhen 518083, P.R.China
⁴ China Veterinary Culture Collection Center, China Institute of Veterinary Drug Control, Beijing 100081, P.R.China

Received:2019-01-22 Revised:2019-01-31 Online:2019-07-01 Published:2019-07-01
Contact: Correspondence HUANG Yin-hua, E-mail: cauhyh@cau.edu.cn
About author:* These authors contributed equally to this study.
Supported by:
The sequencing of the duck transcriptomes was funded by the National Natural Science Foundation of China (31471176) and the Fundamental Research Funds for the Central Universities, China (15054034).

摘要/Abstract

Abstract:

H5N1 influenza represents one of the great challenges to public health. Some H5N1 viruses (i.e., A/goose/Hubei/65/05, GS/65) are weakly pathogenic, while the others (i.e., A/duck/Hubei/49/05, DK/49) are highly pathogenic to their natural hosts. Here, we performed brain and spleen transcriptomic analyses of control ducks and ones infected by the DK/49 or the GS/65 H5N1 virus. We demonstrated that, compared to the GS/65 virus, the DK/49 virus infection changed more numerous immune genes’ expression and caused continuous increasing of immune pathways (i.e., RIG-I and MDA5) in ducks. We found that both H5N1 virus strains might escape or subvert host immune response through affecting alternative translation of immune genes, while the DK/49 virus seemed to induce alternative translation of more immune genes than the GS/65 virus. We also identified five co-expressional modules associated with H5N1 virus replication through the weight correlation network analysis (WGCNA). Moreover, we first demonstrated that the duck BCL2L15 and DCSTAMP in one of these five modules inhibited both the highly pathogenic and weakly pathogenic H5N1 virus replication efficiently. These analyses, in combination with our comprehensive transcriptomic data, provided global view of the molecular architecture for the interaction between host and H5N1 viruses.

Key words: duck , innate immune genes , H5N1 influenza viruses , transcriptomes

. [J]. Journal of Integrative Agriculture, 2019, 18(7): 1460-1472.

HUANG Yin-hua, FENG Hua-peng, HUANG Li-ren, YI Kang, RONG En-guang, CHEN Xiao-yun, LI Jian-wen, WANG Zeng, ZHU Peng-yang, LIU Xiao-juan, WANG Xiao-xue, HU Jia-xiang, LIU Xin, CHEN Hua-lan, WANG Jun.... Transcriptomic analyses reveal new genes and networks response to H5N1 influenza viruses in duck (Anas platyrhynchos)[J]. Journal of Integrative Agriculture, 2019, 18(7): 1460-1472.

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Transcriptomic analyses reveal new genes and networks response to H5N1 influenza viruses in duck (Anas platyrhynchos)

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