Differential responses of root growth to nutrition with different ammonium/nitrate ratios involve auxin distribution in two tobacco cultivars

doi:10.1016/S2095-3119(19)62595-5

摘要/Abstract

Abstract: Nitrogen (N), the major forms of which are nitrate (NO₃^–) and ammonium (NH₄⁺), plays an important role in plant growth and mediation of root development. However, the role of auxin in root growth in response to different NH₄⁺/NO₃^– ratios remains unclear. Two tobacco cultivars (Nicotiana tabacum L.) were adopted in this study, which displayed variant growth features under the situations with sole NO₃^– nutrition ratio (NH₄⁺/NO₃^– ratio: 0/100), low NO₃^– nutrition ratio (NH₄⁺/NO₃^–ratio: 97/3), and optimal NH₄⁺/NO₃^– ratio (50/50). We investigated the effects of the different NH₄⁺/NO₃^–ratios on the formation and elongation of lateral roots (LRs), auxin concentration, DR5::GUS expression, 3H-labeled indole acetic acid ([3H]IAA) transport, and the expression of six PIN genes in tobacco roots. We also examined the effects of exogenous auxin and a transport inhibitor on LRs growth. The results are shown as follows, compared to optimal N nutrition conditions, the biomass and nitrogen (N) accumulation were largely reduced by sole and low NO₃^– nutrition treatment in NC89, but no difference was observed in Zhongyan 100. In most cases, sole and low NO₃^– nutrition impaired the elongation and formation of first-order lateral roots (1° LRs), only in NC89, thus reducing the root growth. IAA concentration and DR5::GUS expression levels decreased in roots when NC89 was subjected to sole and low NO₃^– nutrition media, suggesting that different NH₄⁺/NO₃^– ratios affect the transport of auxin from leaves to roots. Results were similar following exogenous NAA application to low NO₃^– nutrition treated seedlings. Based on direct [3H]IAA transport measurement, the transport of polar auxin from shoots to roots decreased due to low NO₃^–nutrition. PIN4 expression levels were markedly decreased in roots of NC89 by sole and low NO₃^– nutrition, while they were unaffected in Zhongyan 100 roots. Overall, our findings suggest that LRs formation in tobacco seedlings is regulated by NH₄⁺/NO₃^– ratios via modifying polar transport of auxin.

Key words: auxin , lateral root , formation and elongation , tobacco , NH₄⁺/NO₃^– ratio

. [J]. Journal of Integrative Agriculture, 2019, 18(12): 2703-2715.

MENG Lin, DONG Jian-xin, WANG Shu-sheng, SONG Ke, LING Ai-fen, YANG Jin-guang, XIAO Zhi-xin, LI Wei, SONG Wen-jing, LIANG Hong-bo. Differential responses of root growth to nutrition with different ammonium/nitrate ratios involve auxin distribution in two tobacco cultivars[J]. Journal of Integrative Agriculture, 2019, 18(12): 2703-2715.

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