Identification of commercial cultivars of Agaricus bisporus in China using genome-wide microsatellite markers

doi:10.1016/S2095-3119(18)62126-4

Journal of Integrative Agriculture ›› 2019, Vol. 18 ›› Issue (3): 580-589.DOI: 10.1016/S2095-3119(18)62126-4

收稿日期:2018-01-29 出版日期:2019-03-01 发布日期:2019-03-07

Identification of commercial cultivars of Agaricus bisporus in China using genome-wide microsatellite markers

WANG Li-ning, GAO Wei, WANG Qiong-ying, QU Ji-bin, ZHANG Jin-xia, HUANG Chen-yang

Key Laboratory of Microbial Resources, Ministry of Agriculture and Rural Affairs/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

Received:2018-01-29 Online:2019-03-01 Published:2019-03-07
Contact: Correspondence HUANG Chen-yang, Tel: +86-10- 82106207, E-mail: huangchenyang@caas.cn
About author:WANG Li-ning, E-mail: wanglining90@126.com, Tel: +86-10- 82106735;
Supported by:
This work was supported by the earmarked fund for China Agriculture Research System (CARS20) and the Crops Germplasm Resources Preservation Project, the Ministry of Agriculture and Rural Affairs of China.

摘要/Abstract

Abstract: Agaricus bisporus is one of the most widely cultivated mushrooms in the world. Commercial cultivars are usually phenotypically alike and easy to be copied by isolating tissue cultures. This brings great challenges to distinguish different cultivars and to protect new varieties. Thus, techniques for the accurate identification of cultivars are essentially required. In this study, we accurately identified 11 commercial cultivars of A. bisporus released in China by using microsatellite (SSR, simple sequence repeat) markers. SSR markers were developed by mining the genome sequence. A total of 3 134 SSRs were identified, of which 1 490 SSRs were distributed in gene models, and 1 644 in the intergenic regions. A total of 17 polymorphic primer pairs were developed and SSR fingerprints were constructed for all the commercial cultivars. These SSR markers generated a total of 73 alleles, with an average of 4.29 per locus. Specifically, the primer combination of AB_SSR_2341 and AB_SSR_2590 could distinguish all the 11 commercial cultivars. The similarity coefficients of the 11 commercial cultivars were between 0.56 and 0.95 indicating that some of them were close related. Our results provide an efficient technique for the identification of A. bisporus cultivars in China, which can also facilitate the marker-assisted breeding in the future.

Key words: Agaricus bisporus , SSR , strain identification , fingerprint

. [J]. Journal of Integrative Agriculture, 2019, 18(3): 580-589.

WANG Li-ning, GAO Wei, WANG Qiong-ying, QU Ji-bin, ZHANG Jin-xia, HUANG Chen-yang. Identification of commercial cultivars of Agaricus bisporus in China using genome-wide microsatellite markers[J]. Journal of Integrative Agriculture, 2019, 18(3): 580-589.

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Identification of commercial cultivars of Agaricus bisporus in China using genome-wide microsatellite markers

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