Molecular mapping of stripe rust resistance gene YrH9017 in wheat-Psathyrostachys huashanica introgression line H9017-14-16-5-3

doi:10.1016/S2095-3119(18)62048-9

Journal of Integrative Agriculture ›› 2019, Vol. 18 ›› Issue (1): 108-114.DOI: 10.1016/S2095-3119(18)62048-9

所属专题：植物抗病遗传合辑Plant Disease-resistance Genetics

收稿日期:2018-04-03 出版日期:2019-01-01 发布日期:2019-01-02

Molecular mapping of stripe rust resistance gene YrH9017 in wheat-Psathyrostachys huashanica introgression line H9017-14-16-5-3

MA Dong-fang^{1, 2*}, HOU Lu^1*, SUN Cai^2*, ZHANG Xing², YIN Jun-liang², GUO Qing-yun¹, ZHU Yong-xing²

¹ Qinghai Academy of Agriculture and Forestry Sciences, Qinghai University/Key Laboratory of Agricultural Integrated Pest Management, Qinghai Province/State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, P.R.China
² Hubei Collaborative Innocvtion Center for Grain Industry/College of Agriculture, Yangtze University, Jingzhou 434023, P.R.China

Received:2018-04-03 Online:2019-01-01 Published:2019-01-02
Contact: Correspondence GUO Qing-yun, Tel: +86-971-5311056, E-mail: guoqingyunqh@163.com; ZHU Yong-xing, Tel: +86-716-8066302, E-mail: xbnlzyx@163.com * These authors contributed equally to this study.
About author:MA Dong-fang, E-mail: madongfang1984@163.com; HOU Lu, E-mail: mantou428@163.com; SUN Cai, E-mail: SUNCAI1211 @163.com;
Supported by:
The research was funded by the National Natural Science Foundation of China (31660513, 31501620 and 31701911)and the Provincial Natural Science Foundation of Qinghai, China (2017-ZJ-793). Finally, we thank Prof. Qiao Yongli (Shanghai Normal University, China) for beneficial comments on the initial project design and data analysis.

摘要/Abstract

Abstract:

Several new stripe rust pathogen races emerged in the wheat growing regions of China in recent years. These races were virulent to most of the designated wheat seedling resistance genes. Thus, it is necessary and worthwhile to identify new valuable resistant materials for the sake of diversifying resistant sources, pyramiding different resistance genes and achieving durable resistance. Here, we identified the resistance gene, temporarily designated as YrH9017, in wheat-Psathyrostachys huashanica introgression line H9017-14-16-5-3. A total of 146 F₂ plants and their derived F_2:3 families in a cross of Mingxian 169
and H9017-14-16-5-3 were used to evaluate seedling stripe rust response and as a mapping population. Finally, we constructed a genetic map including eight simple sequence repeat (SSR) markers and expressed sequence tag (EST) markers. YrH9017 was located on the long arm of chromosome 2A and closely linked with two EST-sequence tagged site (EST-STS) markers BG604577 and BE471201 at 1.3 and 1.8 cM distance, respectively. The two closest markers could be used for marker-assisted selection of YrH9017 in breeding.

Key words: Puccinia striiformis , Psathyrostachys huashanica , stripe rust , molecular mapping , resistance gene

. [J]. Journal of Integrative Agriculture, 2019, 18(1): 108-114.

MA Dong-fang, HOU Lu, SUN Cai, ZHANG Xing, YIN Jun-liang, GUO Qing-yun, ZHU Yong-xing. Molecular mapping of stripe rust resistance gene YrH9017 in wheat-Psathyrostachys huashanica introgression line H9017-14-16-5-3[J]. Journal of Integrative Agriculture, 2019, 18(1): 108-114.

参考文献

Bariana H S, McIntosh R A. 1993. Cytogenetic studies in wheat XV. Location of rust resistance genes in VPM1 and their genetic linkage with other disease resistance genes in chromosome 2A. Genome, 36, 476–482.
Bansal U K, Hayden M J, Keller B, Wellings C R, Park R F, Bariana H S. 2009. Relationship between wheat rust resistance genesYr1 and Sr48 and a microsatellite marker. Plant Pathology, 58, 1039–1043.
Can C, He Z H, Lu J L, Li J, Ren Y, Ma C X, Xia X C. 2016. Molecular mapping of stripe rust resistance gene YrJ22 in Chinese wheat cultivar Jimai 22. Molecular Breeding, 36, 118.
Cao Z J, Wang X P, Wang M N, Cao S H, Jing J X, Shang H S, Li Z Q, Zhang X Q. 2005. Genetic analysis and molecular markers of a novel stripe rust resistance gene YrHua in wheat originated from Psathyrostachys huashanica Keng. Acta Genetica Sinica, 32, 738.
Chen W Q, Wu L R, Liu T G, Xu S C, Jin S L, Peng Y L, Wang B T. 2009. Race dynamics, diversity, and virulence evolution in Puccinia striiformis f. sp. tritici, the causal agent of wheat stripe rust in China from 2003 to 2007. Plant Disease, 93, 1093–1101.
Chen X M, Line R F, Leung H. 1998. Genome scanning for resistance-gene analogs in rice, barley, and wheat by high-resolution electrophoresis. Theoretical and Applied Genetics, 97, 345–355.
Chen X M, Penman L, Wan A M, Cheng P. 2010. Virulence races of Puccinia striiformis f. sp. tritici in 2006 and 2007 and development of wheat stripe rust and distributions, dynamics, and evolutionary relationships of races from 2000 to 2007 in the United States. Canadian Journal of Plant Pathology, 32, 315–333.
Eriksen L, Afshari F, Christiansen M J, McIntosh R A, Jahoor A, Wellings C R. 2004. Yr32 for resistance to stripe (yellow) rust present in the wheat cultivar Carstens V. Theoretical and Applied Genetics, 108, 567–575.
Jiao M, Tan C, Wang L, Guo J, Zhang H C, Kang Z S, Guo J. 2017. Basidiospores of Puccinia striiformis f. sp. tritici succeed to infect barberry, while urediniospores are blocked by non-host resistance. Protoplasma, 254, 1–10.
Jing J X, Fu J, Yuan H X, Wang M N, Shang H S, Li Z Q. 1999. A preliminary study on heredity of the resistance to stripe rust in three wild relatives of wheat. Acta Phytopathol Sinica, 29, 147–150. (in Chinese)
Kosambi D D. 1944. The estimation of map distances from recombination values. Annals of Human Genetics, 12, 172–175.
Lander E S, Green P, Abrahamson J, Barlow A, Daly M J, Lincoln S E, Newberg L A. 1987. Mapmaker: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics, 1, 174–181.
Li G Q, Li Z F, Yang W Y, Zhang Y, He Z H, Xu S C, Singh R P, Qu Y Y, Xia X C. 2006. Molecular mapping of stripe rust resistance gene YrCH42 in Chinese wheat cultivar Chuanmai 42 and its allelism with Yr24 and Yr26. Theoretical and Applied Genetics, 112, 1434–1440.
Li Q, Chao K X, Li, Q, Zhang Y, Jing J X, Wang B T, Kang Z S. 2016. Genetic analysis and molecular mapping of a stripe rust resistance gene in wheat-Leymus mollis, translocation line m8926-2. Crop Protection, 86, 17–23.
Li Q, Huang J, Hou L, Liu P, Jing J X, Wang B T, Kang Z S. 2012. Genetic and molecular mapping of stripe rust resistance gene in wheat-Psathyrostachys huashanica translocation line H9020-1-6-8-3. Plant Disease, 96, 1482–1487.
Liu J, Chang Z J, Zhang X J, Yang Z J, Li X, Jia J Q, Zhan H X, Guo H J, Wang J M. 2013. Putative Thinopyrum intermedium-derived stripe rust resistance gene Yr50 maps on wheat chromosome arm 4BL. Theoretical and Applied Genetics, 126, 265–274.
Liu T G, Peng Y L, Chen W Q, Zhang Z Y. 2010. First detectionof virulence in Puccinia striiformis f. sp. tritici in China to resistance genes Yr24 (=Yr26) present in wheat cultivar Chuanmai 42. Plant Disease, 94, 1163.
Ma D F, Fang Z W, Yin J L, Chao K X, Jing J X, Li Q, Wang B T. 2016. Molecular mapping of stripe rust resistance gene YrHu derived from Psathyrostachys huashanica. Molecular Breeding, 36, doi: 10.1007/s11032-016-0487-6
Ma D F, Zhou X L, Hou L, Bai Y B, Li Q, Wang H G, Tang M S, Jing J X. 2013. Genetic analysis and molecular mapping of a stripe rust resistance gene derived from Psathyrostachys huashanica keng in wheat line H9014-121-5-5-9. Molecular Breeding, 32, 365–372.
Rosewarne G M. 2013. Close genetic linkage of genes conferring adult-plant resistance to leaf rust and stripe rust in wheat. Plant Pathology, 41, 523–527.
Somers D J, Isaac P, Edwards K. 2004. A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 109, 1105–1114.
Tang Z X, Li M, Chen L, Wang Y Y, Ren Z L, Fu S L. 2014a. New types of wheat chromosomal structural variations in derivatives of wheat-rye hybrids. PLoS ONE, 9, e110282.
Tang Z X, Li M, Chen L, Wang Y Y, Ren Z L, Fu S L. 2014b. Oligonucleotides replacing the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH analysis. Journal of Applied Genetics, 55, 313–318.
Thiel T, Michalek W, Varshney R K, Graner A. 2003. Exploiting est databases for the development and characterization of gene-derived ssr-markers in barley (Hordeum vulgare L.). Theoretical and Applied Genetics, 106, 411–422.
Wan A M, Chen X M, He Z H. 2007. Wheat stripe rust in China. Australian Journal of Agricultural Research, 58, 605–619.
Wan A M, Zhao Z H, Chen X M, He Z H, Jin S L, Jia Q Z, Yao G, Yang J X, Wang B T, Li G B, Bi Y Q, Yuan Z Y. 2004. Wheat stripe rust epidemic and virulence of Puccinia striiformis f. sp. tritici in China in 2002. Plant Disease, 88, 896–904.
Wang C M, Zhang Y P, Han D J, Kang Z S, Li G P, Cao A Z, Chen P D. 2008. SSR and STS markers for wheat stripe rust resistance gene Yr26. Euphytica, 159, 359–366.
Wang C M, Zheng Q, Li L H, Niu Y C, Wang H B, Li B, Zhang X T, Xu Y F, An D G. 2009. Molecular cytogenetic characterization of a new T2BL 1RS wheat-rye chromosome translocation line resistant to stripe rust and powdery mildew. Plant Disease, 93, 124–129.
Wang M N, Shang H S. 2000. Evaluation of resistance in Psathyrostachys huashaica to wheat take-all fungus. Journal of Northwest Agricultural University, 28, 69–71. (in Chinese)
Yang X F, Wang C Y, Li X, Chen C H, Tian Z R, Wang Y J, Ji W Q. 2015. Development and molecular cytogenetic identification of a novel wheat-Leymus mollis Lm#7Ns (7D) disomic substitution line with stripe rust resistance. PLoS ONE, 10, e0140227.
Yao Q, Wang Y, He M M, Li Y, Zhou X L, Wang B T, Jing J X. 2010. SSR molecular mapping of stripe rust resistance gene of wheat translocation line H9020-20-12-1-8 derived from Psathyrostachys huashanica Keng. Journal of Agricultural Biotechnology, 18, 676–681.
Zhao J X, Chen X H, Wang X L, Wu J, Fu J, He B R, Song Y Z, Sun Z G. 2003. C-Banding identification of alien substitution linesand alien additional lines in Triticum-Psathyrostachys. Journal of Northwest Sci-Tech University of Agriculture and Forestry (Natural Science Edition), 31, 1–4. (in Chinese)

Molecular mapping of stripe rust resistance gene YrH9017 in wheat-Psathyrostachys huashanica introgression line H9017-14-16-5-3

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics