Identification and QTL mapping of Z550, a rice backcrossed inbred line with increased grains per panicle

doi:10.1016/S2095-3119(18)61996-3

Journal of Integrative Agriculture ›› 2019, Vol. 18 ›› Issue (3): 526-531.DOI: 10.1016/S2095-3119(18)61996-3

收稿日期:2018-01-19 出版日期:2019-03-01 发布日期:2019-03-07

Identification and QTL mapping of Z550, a rice backcrossed inbred line with increased grains per panicle

WANG Shi-ming, CUI Guo-qing, WANG Hui, MA Fu-ying, XIA Sai-sai, LI Yun-feng, YANG Zheng-lin, LING Ying-hua, ZHANG Chang-wei, HE Guang-hua, ZHAO Fang-ming

Rice Research Institute, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, P.R.China

Received:2018-01-19 Online:2019-03-01 Published:2019-03-07
Contact: Correspondence ZHAO Fang-ming, Tel: +86-23-68250486, E-mail: zhaofangming2004@163.com
About author: WANG Shi-ming, E-mail: wangshiming1993@126.com;
Supported by:
The study was supported by the National Key R&D Program of China (2017YFD0100202), the Chongqing Science and Technology Commission Special Project, China (cstc2016shms-ztzx0017), and the Southwestern University Basic Operating Expenses Special Innovation Team Project, China (XDJK2017A004).

摘要/Abstract

Abstract:

An elite backcrossed inbred line Z550 with increased grains per panicle was identified from advanced backcrosses between Nipponbare and Xihui 18 by simple sequence repeat (SSR) marker-assisted selection (MAS). Z550 carries 13 substitution segments distributed on chromosomes 1, 6, 7, 8, 9, 10, and 12, with an average substitution length of 1.68 Mb. Compared with the Nipponbare parental line, plant height, panicle length, spikelets per panicle, grains per panicle, and grain weight for Z550 were significantly increased. While the grain width of Z550 was significantly narrower, and the seed setting ratio (81.43%) was significantly lower than that of Nipponbare, it is still sufficient for breeding purposes. Quantitative trait loci (QTLs) mapping for important agronomic traits was conducted with the F₂ population derived from Nipponbare crossed with Z550 using the restricted maximum likelihood (REML) method. A total of 16, including 12 previously unreported QTLs were detected, with contribution rates ranging from 1.46 to 10.49%. Grains per panicle was controlled by 8 QTLs, 5 of which increased number of grains whereas 3 decreased it. qGPP-1, with the largest contribution (10.49%), was estimated to increase grains per panicle by 30.67, while qGPP-9, with the minimum contribution rate (2.47%), had an effect of increasing grains per panicle by 15.79. These results will be useful for further development of single segment substitution lines with major QTLs, and for research of their molecular functions via QTL cloning.

Key words: rice , backcrossed inbred line , yield , QTL mapping

. [J]. Journal of Integrative Agriculture, 2019, 18(3): 526-531.

WANG Shi-ming, CUI Guo-qing, WANG Hui, MA Fu-ying, XIA Sai-sai, LI Yun-feng, YANG Zheng-lin, LING Ying-hua, ZHANG Chang-wei, HE Guang-hua, ZHAO Fang-ming. Identification and QTL mapping of Z550, a rice backcrossed inbred line with increased grains per panicle[J]. Journal of Integrative Agriculture, 2019, 18(3): 526-531.

参考文献

Abe Y, Mieda K, Ando T, Kono I, Yano M, Kitano H, Iwasaki Y. 2010. The small and round seed1 (SRS1/DEP2) gene is involved in the regulation of seed size in rice. Genes & Genetic Systems, 85, 327.
Ashikari M, Sakakibara H, Lin S Y, Yamamoto T, Takashi T, Nishimura A, Enrique R, Angeles Q Q, Kitano H, Matsuoka M. 2005. Cytokinin oxidase regulates rice grain production. Science, 309, 741–745.
Deshmukh R, Singh A, Jain N, Anand S, Gacche R, Singh A, Gaikwad K, Sharma T, Mohapatra T, Singh N. 2010. Identification of candidate genes for grain number in rice (Oryza sativa L.). Functional & Integrative Genomics, 10, 339–347.
Huang X, Qian Q, Liu Z, Sun H, He S Y, Luo D, Xia G M, Chu C C, Li J Y, Fu X D. 2009. Natural variation at the DEP1 locus enhances grain yield in rice. Nature Genetics, 41, 494.
Kobayashi K, Maekawa M, Miyao A, Hirochika H, Kyozuka J. 2010. PANICLE PHYTOMER2 (PAP2), encoding a SEPALLATA subfamily MADS-box protein, positively controls spikelet meristem identity in rice. Plant & Cell Physiology, 51, 47–57.
Li W, Wu J, Weng S, Zhang Y J, Zhang D P, Shi C H. 2010. Identification and characterization of dwarf 62, a loss-of-function mutation in DLT/OsGRAS-32 affecting gibberellin metabolism in rice. Planta, 232, 1383–1396.
Liu T, Mao D, Zhang S, Xu C, Xing Y. 2009. Fine mapping SPP1, a QTL controlling the number of spikelets per panicle, to a BAC clone in rice (Oryza sativa). Theoretical and Applied Genetics, 118, 1509–1517.
McCouch S R, Kochert G, Yu Z H. 1988. Molecular mapping of rice chromosome. Theoretical and Applied Genetics, 76, 148–159
Moralesramos Á I, Li Y H, Hilfiker M, Mecom J S, Eidam P, Shi D C, Tseng P, Brooks C, Zhang D, Wang N, Jaworski J, Morrow D, Fries H, Edwards R, Jin J. 2011. Structure-activity relationship studies of novel 3-oxazolidinedione-6-naphthyl-2-pyridinones as potent and orally bioavailable EP3 receptor antagonists. Bioorganic & Medicinal Chemistry Letters, 21, 2806–2811.
Paterson A H, Damon S, Hewitt J D, Zamir D, Rabinowitch H D, Lincoln S E, Lander E S, Tanksley S D. 1991. Mendelian factors underlying quantitative traits in tomato: Comparison across species, generations, and environments. Genetics, 127, 181–197.
Piao R, Jiang W Z, Ham T H, Choi M S, Qiao Y, Chu S H, Park J, Woo M O, Jin Z X, An G, Lee J Y, Kohet H J. 2009. Map-based cloning of the ERECT PANICLE 3 gene in rice. Theoretical and Applied Genetics, 119, 1497–506.
SAS Institute. 2009. SAS/STAT: Users’ guide. version 9.3. http://suport.sus.com/publishing
Tan Y F, Li J X, Yu S B, Xu C G, Zhang Q F, Xing Y Z. 2000. Genetic bases of appearance quality of rice grains in Shanyou 63, an elite rice hybrid. Theoretical and Applied Genetics, 101, 823–829.
Xing Y Z, Tang W J, Xue W Y, Xu C G, Zhang Q. 2008. Fine mapping of a major quantitative trait loci, qSSP7, controlling the number of spikelets per panicle as a single mendelian factor in rice. Theoretical and Applied Genetics, 116, 789–796.
Xue W Y, Xing Y Z, Weng X Y, Zhao Y, Tang W J, Wang L, Zhou H J, Yu S B, Xu C G, Li X H, Zhang Q F. 2008. Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice. Nature Genetics, 40, 761–767.
Yu H, Murchie E H, Gonzálezcarranza Z H, Pyke K A, Roberts J A. 2015. Decreased photosynthesis in the erect panicle 3
(EP3) mutant of rice is associated with reduced stomatal conductance and attenuated guard cell development. Journal of Experimental Botany, 66, 1543.
Zhao F M, Tan Y, Zheng L Y, Zhou K, He G H, Ling Y H, Zhang L H, Xu S Z. 2016. Identification of rice chromosome segment substitution line Z322-1-10 and mapping QTLs for agronomic traits from the F3 population. Cereal Research Communication, 44, 1–11.
Zhou Y, Zhu J, Li Z, Yi C, Liu J, Zhang H, Tang S, Gu M, Liang G. 2009. Deletion in a quantitative trait gene qPE9-1 associated with panicle erectness improves plant architecture during rice domestication. Genetics, 183, 315.
Zhu K, Tang D, Yan C, Chi Z C, Yu H X, Chen J M, Liang J S, Gu M H, Cheng Z K. 2010. ERECT PANICLE2 encodes a novel protein that regulates panicle erectness in indica rice. Genetics, 184, 343.

Identification and QTL mapping of Z550, a rice backcrossed inbred line with increased grains per panicle

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics