Microsatellites reveal strong genetic structure in the common cutworm, Spodoptera litura

doi:10.1016/S2095-3119(18)61989-6

Journal of Integrative Agriculture ›› 2019, Vol. 18 ›› Issue (3): 636-643.DOI: 10.1016/S2095-3119(18)61989-6

所属专题：昆虫生防和生态合辑Insect Biocontrol and Ecology

收稿日期:2017-12-21 出版日期:2019-03-01 发布日期:2019-03-07

Microsatellites reveal strong genetic structure in the common cutworm, Spodoptera litura

WU Huai-heng^{1, 2}, WAN Peng², HUANG Min-song², LEI Chao-liang¹

¹ Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, Huazhong Agricultural University, Wuhan 430070, P.R.China
² Key Laboratory of Integrated Pest Management on Crops in Central China, Institute of Plant Protection and Soil Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, P.R.China

Received:2017-12-21 Online:2019-03-01 Published:2019-03-07
Contact: Correspondence LEI Chao-liang, E-mail: ioir@mail.hzau.edu.cn
Supported by:
The work was funded by the Youth Foundation of Hubei Academy of Agricultural Sciences, China (2016NKYJJ11).

摘要/Abstract

Abstract:

The common cutworm, Spodoptera litura (Lepidoptera: Noctuidae), is a voracious agricultural pest. To increase understanding of the migration patterns and genetic diversity of different geographic populations of this species, we analyzed genetic variation in nine microsatellite loci among 576 individuals collected from 17 locations in China and one in Myanmar during 2011–2012. We successfully identified 162 alleles, with an average of 18 alleles per locus and a range of 5 to 34. The mean observed heterozygosity of the 18 populations ranged from 0.18 to 0.98, and the expected heterozygosity ranged from 0.19 to 0.89. For the nine microsatellite markers studied, polymorphism information content ranged from 0.18 to 0.88 (mean=0.64). We found low genetic differentiation among the 18 populations (mean F-statistics (F_ST)=0.05) and high genetic diversity among individuals. Principle coordinates analysis indicated no significant correlation between geographic and genetic distance (r=0.04). The value of N_m (N_m>4) estimated using coalescent-based methods suggests strong gene flow with migration. The nine microsatellite markers identified in this study will be beneficial for further investigation of migration patterns and genetic diversity in S. litura.

Key words: Spodoptera litura , microsatellite , genetic diversity

. [J]. Journal of Integrative Agriculture, 2019, 18(3): 636-643.

WU Huai-heng, WAN Peng, HUANG Min-song, LEI Chao-liang. Microsatellites reveal strong genetic structure in the common cutworm, Spodoptera litura[J]. Journal of Integrative Agriculture, 2019, 18(3): 636-643.

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Microsatellites reveal strong genetic structure in the common cutworm, Spodoptera litura

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