Identification, characterization and full-length sequence analysis of a novel endornavirus in common sunflower (Helianthus annuus L.)

doi:10.1016/S2095-3119(18)61963-X

Journal of Integrative Agriculture ›› 2018, Vol. 17 ›› Issue (10): 2281-2291.DOI: 10.1016/S2095-3119(18)61963-X

收稿日期:2017-12-27 出版日期:2018-10-01 发布日期:2018-09-29

Identification, characterization and full-length sequence analysis of a novel endornavirus in common sunflower (Helianthus annuus L.)

LIU Wen-wen¹, XIN Min¹, CAO Meng-ji², QIN Meng³, LIU Hui³, ZHAO Shou-qi³, WANG Xi-feng¹

¹ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
² National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing 400712, P.R.China
³ National Agricultural Technology Extension and Service Center, Beijing 100125, P.R.China

Received:2017-12-27 Online:2018-10-01 Published:2018-09-29
Contact: Correspondence WANG Xi-feng, E-mail: xfwang@ippcaas.cn
About author:LIU Wen-wen, E-mail: wwliu@ippcaas.cn;
Supported by:
This research was supported by the Inter-Governmental S&T Cooperation Proposal between China and Czech Republic (2016YFE0131000) and the Beijng Nova Program, China (Z171100001117036).

摘要/Abstract

Abstract:

To identify the possible quarantine viruses in seven common sunflower varieties imported from the United States of America and the Netherlands, we tested total RNAs extracted from the leaf tissues using next-generation sequencing of small RNAs. After analysis of small RNA sequencing data, no any quarantine virus was found, but a double-stranded RNA (dsRNA) molecule showing typical genomic features of endornavirus was detected in two varieties, X3939 and SH1108. Full-length sequence and phylogenetic analysis showed that it is a novel endornavirus, temporarily named as Helianthus annuus alphaendornavirus (HaEV). Its full genome corresponds to a 14 662-bp dsRNA segment, including a 21-nt 5´ untranslated region (UTR), 3´ UTR ending with the unique sequence CCCCCCCC and lacking a poly(A) tail. An open reading frame (ORF) that encodes a deduced 4 867 amino acids (aa) polyprotein with three domains: RdRP, Hel and UGT (UDP-glycosyltransferase). HaEV mainly distributed in the cytoplasm but less in the nucleus of leaf cells by fluorescence in situ hybridization (FISH) experiment. This virus has a high seed infection rate in the five varieties, X3907, X3939, A231, SH1108 and SR1320. To our knowledge, this is the first report about the virus of the family Endornaviridae in the common sunflower.

Key words: common sunflower (Helianthus annuus) , next-generation sequencing (NGS) , double-stranded RNA , Endornaviridae

. [J]. Journal of Integrative Agriculture, 2018, 17(10): 2281-2291.

LIU Wen-wen, XIN Min, CAO Meng-ji, QIN Meng, LIU Hui, ZHAO Shou-qi, WANG Xi-feng. Identification, characterization and full-length sequence analysis of a novel endornavirus in common sunflower (Helianthus annuus L.)[J]. Journal of Integrative Agriculture, 2018, 17(10): 2281-2291.

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Identification, characterization and full-length sequence analysis of a novel endornavirus in common sunflower (Helianthus annuus L.)

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