Up-regulation of a homeodomain-leucine zipper gene HD-1 contributes to trichome initiation and development in cotton

doi:10.1016/S2095-3119(18)61914-8

摘要/Abstract

Abstract:

Plant trichomes originate from epidermal cells. In this work, we demonstrated that a homeodomain-leucine zipper (HD-Zip) gene, Gh_A06G1283 (GhHD-1A), was related to the leaf trichome trait in allotetraploid cotton and could be a candidate gene for the T₁ locus. The ortholog of GhHD-1A in the hairless accession Gossypium barbadense cv. Hai7124 was interrupted by a long terminal repeat (LTR) retrotransposon, while GhHD-1A worked well in the hairy accession Gossypium hirsutum acc. T586. Sequence and phylogenetic analysis showed that GhHD-1A belonged to the HD-Zip IV gene family, which mainly regulated epidermis hair development in plants. Silencing of GhHD-1A and its homoeologs GhHD-1D in allotetraploid T586 and Hai7124 could significantly reduce the density of leaf hairs and affect the expression levels of other genes related to leaf trichome formation. Further analysis found that GhHD-1A mainly regulated trichome initiation on the upper epidermal hairs of leaves in cotton, while the up-regulated expression of GhHD-1A in different organs/tissues also altered epidermal trichome development. This study not only helps to unravel the important roles of GhHD-1A in regulating trichome initiation in cotton, but also provides a reference for exploring the different forms of trichome development in plants.

Key words: leaf trichome , map-based cloning , a homeodomain-leucine zipper gene HD-1 , virus-induced gene silencing (VIGS) , functional differentiation

. [J]. Journal of Integrative Agriculture, 2019, 18(2): 361-371.

NIU Er-li, CAI Cai-ping, BAO Jiang-hao, WU Shuang, ZHAO Liang, GUO Wang-zhen. Up-regulation of a homeodomain-leucine zipper gene HD-1 contributes to trichome initiation and development in cotton[J]. Journal of Integrative Agriculture, 2019, 18(2): 361-371.

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