Conidia of one Fusarium solani isolate from a soybean-production field enable to be virulent to soybean and make soybean seedlings wilted

doi:10.1016/S2095-3119(17)61891-4

Journal of Integrative Agriculture ›› 2018, Vol. 17 ›› Issue (09): 2042-2053.DOI: 10.1016/S2095-3119(17)61891-4

所属专题：植物细菌真菌合辑Plant Bacteria/Fungus

收稿日期:2017-12-31 出版日期:2018-09-01 发布日期:2018-08-14

Conidia of one Fusarium solani isolate from a soybean-production field enable to be virulent to soybean and make soybean seedlings wilted

ZHENG Na^1*, ZHANG Liu-ping^1*, GE Feng-yong¹, HUANG Wen-kun¹, KONG Ling-an¹, PENG De-liang¹, LIU Shi-ming^{1, 2}

¹Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
² College of Plant Protection, Hunan Agricultural University, Changsha 410128, P.R.China

Received:2017-12-31 Online:2018-09-01 Published:2018-08-14
Contact: Correspondence LIU Shi-ming, E-mail: smliuhn@yahoo.com
About author:ZHENG Na, E-mail: 476211775@qq.com; ZHANG Liu-ping, E-mail: Liupingz2013@163.com; * These authors contributed equally to this study
Supported by:
This work was financially supported by the Innovation Program and Youth Elite Program of Chinese Academy of Agricultural Sciences.

摘要/Abstract

摘要： Received 13 December, 2017 Accepted 17 January, 2018
.

Abstract:

Fusarium is usually thought to cause soybean root rot, which results in a large quantity of annual yield loss in soybean production, by its secretions including Fusarium toxins and cell wall degrading enzymes, but not by the conidia themselves that do not underlie any virulence so far. Here we report that the conidia of one Fusarium solani isolate are able to be virulent to soybean and make soybean seedlings wilted alone. We isolated them from the wilted plants in a soybean-production field and molecularly identified 17 Fusarium isolates through phylogenetic analysis. Of them, except for one isolate that showed diversity of virulence to different soybeans (virulent to one soybean whereas avirulent to another soybean), the others were all virulent to the two tested soybeans: both conidia cultures and secretions could make soybean seedlings wilted at 5 days post infection, and their virulence had dosage effects that only conidia cultures of at least 5×106 conidia mL^–1 could show virulence to soybean; however, the sole conidia of the F. solani isolate #4 also exhibited virulence to soybean and could make soybean seedlings wilted. Finally, we developed the specific cleaved amplified polymorphic sequences (CAPS) markers to easily differentiate Fusarium isolates. The isolate #4 in this work will likely be used to investigate the new mechanism of virulence of Fusarium to soybean.

Key words: Fusarium , soybean root rot , conidia , secretions , virulence , cleaved amplified polymorphic sequences (CAPS) marker

. [J]. Journal of Integrative Agriculture, 2018, 17(09): 2042-2053.

ZHENG Na, ZHANG Liu-ping, GE Feng-yong, HUANG Wen-kun, KONG Ling-an, PENG De-liang, LIU Shi-ming. Conidia of one Fusarium solani isolate from a soybean-production field enable to be virulent to soybean and make soybean seedlings wilted[J]. Journal of Integrative Agriculture, 2018, 17(09): 2042-2053.

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Conidia of one Fusarium solani isolate from a soybean-production field enable to be virulent to soybean and make soybean seedlings wilted

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