Development of EST-PCR markers specific to the long arm of chromosome 6V of Dasypyrum villosum

doi:10.1016/S2095-3119(17)61866-5

Journal of Integrative Agriculture ›› 2018, Vol. 17 ›› Issue (08): 1720-1726.DOI: 10.1016/S2095-3119(17)61866-5

收稿日期:2017-09-01 出版日期:2018-08-01 发布日期:2018-08-01

Development of EST-PCR markers specific to the long arm of chromosome 6V of Dasypyrum villosum

SUN Hao-jie, SONG Jing-jing, XIAO Jin, XU Tao, WEI Xing, YUAN Chun-xia, CAO Ai-zhong, XING Liping, WANG Hai-yan, WANG Xiu-e

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/Cytogenetics Institute, Nanjing Agricultural University/Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP), Nanjing 210095, P.R.China

Received:2017-09-01 Online:2018-08-01 Published:2018-08-01
Contact: Correspondence WANG Hai-yan, Tel: +86-25-84395308, Fax: +86-25-84395344, E-mail: hywang@njau.edu.cn; WANG Xiu-e, E-mail: xiuew@njau.edu.cn
Supported by:
This research was supported by the National Key Research and Development Program of China (2016YFD0102001), the National Natural Science Foundation of China (31571653, 31771782, 31201204), the Technology Support Program of Jiangsu Province, China (BE2013439), the Fundamental Research Funds for the Central Universities, China (KYZ201403), the Jiangsu Agricultural Science and Technology Innovation Fund, China (CX151001), the Program of Introducing Talents of Discipline to Universities, China (B08025), the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (PAPD), and the Six Talent Peaks Project in Jiangsu Province, China.

摘要/Abstract

摘要： Received 1 September, 2017 Accepted 13 December, 2017

Abstract:

Expressed sequence tags-derived polymerase chain reaction (EST-PCR) molecular markers specific for alien chromosomes can be used to not only monitor the introgressed alien chromatin in wheat background, but also provide the evidence of the syntenic relationship between homoeologous chromosomes. In the present study, in order to develop high density and evenly distributed molecular markers specific for chromosome 6VL of Dasypyrum villosum, 297 primer pairs were designed based on the expressed sequence tags (EST) sequences, which were previously mapped in different bins of the long arms of wheat homoeologous 6AL, 6BL, and 6DL. By using the Triticum aestivum, D. villosum, T. durum-D. villosum amphiploid, and T. aestivum-D. villosum alien chromosome lines involving chromosome 6V, it was found that 32 (10.77%) primers could amplify specific bands for chromosome 6V, and 31 could be allocated to chromosome arm 6VL. These 6VL specific markers provided efficient tools for the characterization of structural variation involving the chromosome 6VL in common wheat background as well as for the selection of useful genes located on 6VL in breeding programs.

Key words: Dasypyrum villosum , molecular marker , common wheat , alien chromosome line

. [J]. Journal of Integrative Agriculture, 2018, 17(08): 1720-1726.

SUN Hao-jie, SONG Jing-jing, XIAO Jin, XU Tao, WEI Xing, YUAN Chun-xia, CAO Ai-zhong, XING Liping, WANG Hai-yan, WANG Xiu-e. Development of EST-PCR markers specific to the long arm of chromosome 6V of Dasypyrum villosum[J]. Journal of Integrative Agriculture, 2018, 17(08): 1720-1726.

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Development of EST-PCR markers specific to the long arm of chromosome 6V of Dasypyrum villosum

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