Journal of Integrative Agriculture ›› 2018, Vol. 17 ›› Issue (03): 712-715.DOI: 10.1016/S2095-3119(17)61860-4

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  • 收稿日期:2017-09-17 出版日期:2018-03-20 发布日期:2018-03-01

Complete genome sequences of four isolates of Citrus leaf blotch virus from citrus in China

LI Ping1, 2, LI Min1, 2, ZHANG Song1, 2, WANG Jun1, 2, YANG Fang-yun1, CAO Meng-ji1, 2, LI Zhong-an1, 2      

  1. 1 National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing 400712, P.R.China
    2 College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, P.R.China
  • Received:2017-09-17 Online:2018-03-20 Published:2018-03-01
  • Contact: Correspondence LI Zhong-an, Tel: +86-23-68349007, Fax: +86-23-68349592, E-mail: zhongan@cric.cn; CAO Meng-ji, Tel: +86-23-68349002, Fax: +86-23-68349592, E-mail: caomengji@cric.cn
  • About author:LI Ping, E-mail: liping920902@163.com;
  • Supported by:

    This research was supported by the National Natural Science Foundation of China (31501611), the Chongqing Research Program of Basic Research and Frontier Technology, China (cstc2017jcyjBX0016), the Chongqing Science and Technology Commission Project, China (cstc2016shms-ztzx80003), the Fundamental Research Funds for the Central Universities, China (XDJK2016B21, SWU116012), and the Special Fund for Agro-scientific Research in the Public Interest, China (201203076-01).

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Abstract: Citrus leaf blotch virus (CLBV) is a member of the genus Citrivirus, in the family Betaflexiviridae.  It has been reported CLBV could infect kiwi, citrus and sweet cherry in China.  Of 289 citrus samples from six regions of China, 15 were detected to be infected with CLBV in this study.  The complete genome of four isolates of CLBV was obtained from Reikou in Sichuan (CLBV-LH), Yura Wase in Zhejiang (CLBV-YL), Bingtangcheng in Hunan (CLBV-BT), Fengjie 72-1 in Chongqing (CLBV-FJ), respectively.  While they all represented 8 747 nucleotides in monopartite size, excluding the poly(A) tail, each of the isolates coded three open reading frames (ORFs).  Identity of the four isolates ranged from 98.9 to 99.8% to each other and from 96.8 to 98.1% to the citrus references in GenBank by multiple alignment of genomes.  A phylogenetic tree based on the genome sequences of available CLBV isolates indicated that the four isolates were clustered together, suggesting that CLBV isolates from citrus in China did not have obvious variation.  This is the first report of the complete nucleotide sequences of CLBV isolates infecting citrus in China.

Key words: Citrus leaf blotch virus (CLBV) ,  genome sequence ,  Citrivirus