Transcriptome approach to understand the potential mechanisms of resistant and susceptible alfalfa (Medicago sativa L.) cultivars in response to aphid feeding

doi:10.1016/S2095-3119(17)61843-4

Journal of Integrative Agriculture ›› 2018, Vol. 17 ›› Issue (11): 2518-2527.DOI: 10.1016/S2095-3119(17)61843-4

所属专题：昆虫和植物互作合辑Insect and Plant Interact

收稿日期:2017-07-18 出版日期:2018-11-01 发布日期:2018-11-01

Transcriptome approach to understand the potential mechanisms of resistant and susceptible alfalfa (Medicago sativa L.) cultivars in response to aphid feeding

TU Xiong-bing^1*, ZHAO Hai-long^{1, 2*}, ZHANG Ze-hua¹

¹ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
² College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, P.R.China

Received:2017-07-18 Online:2018-11-01 Published:2018-11-01
Contact: Correspondence ZHANG Ze-hua, Tel: +86-10-82109585, Fax: +86-10-82109569, E-mail: zhangzehua@caas.cn
About author:TU Xiong-bing, E-mail: xbtu@ippcaas.cn; ZHAO Hai-long, E-mail: zhl19930516@163.com; * These authors contributed equally to this study.
Supported by:
This research was funded by the earmarked fund for China Agriculture Research System (CARS-34-07).

摘要/Abstract

Abstract:

Plant breeding for resistance to agricultural pests is an essential element in the development of integrated crop management systems, however, the molecular and genetic mechanisms underlying resistance are poorly understood. In this pilot study, we conducted a transcriptomic analysis of a resistant (R) and susceptible (S) variety of alfalfa, with (+A) or without (–A) aphids (totally four treatments). We used the resistant cultivar Zhongmu 1 and the susceptible cultivar Soca. A total of 3 549 mRNAs were differentially expressed, of which 1 738 up-regulated and 1 307 down-regulated genes were identified in S+A/S–A plants, while 543 up-regulated and 331 down-regulated genes were identified in the R+A/R–A plants. KEGG analysis mapped 112 and 546 differentially expressed genes to 8 and 17 substantially enriched pathways for Zhongmu 1 and Soca, respectively. Six shared pathways were linked to plant resistance traits, including phenylpropanoid biosynthesis associated with salicylic acid synthesis, and linoleic acid metabolism associated with both jasmonic acid and flavonoid biosynthesis. Ultimately, we proposed a preliminary regulatory mechanism of alfalfa cultivar resistance response to aphids feeding based on transcriptome analyses and published documents.

Key words: alfalfa cultivar resistance , aphids , transcriptomic , molecular mechanism

. [J]. Journal of Integrative Agriculture, 2018, 17(11): 2518-2527.

TU Xiong-bing, ZHAO Hai-long, ZHANG Ze-hua. Transcriptome approach to understand the potential mechanisms of resistant and susceptible alfalfa (Medicago sativa L.) cultivars in response to aphid feeding[J]. Journal of Integrative Agriculture, 2018, 17(11): 2518-2527.

参考文献

Alborn H T, Brennan M M, Tumlinson J H. 2003. Differential activity and degradation of plant volatile elicitors in regurgitant of tobacco hornworm (Manduca sexta) larvae. Journal of Chemical Ecology, 29, 1357–1372.
Anders S, Huber W. 2010. Differential expression analysis for sequence count data. Genome Biology, 11, 106.
Brechenmacher L, Nguyen T H N, Zhang N, Jun T H, Xu D, Mian M A, Stacey G. 2015. Identification of soybean proteins and genes differentially regulated in near isogenic lines differing in resistance to aphid infestation. Journal of Proteome Research, 14, 146.
Caarls L, Pieterse C M J, Van Wees S C M. 2015. How salicylic acid takes transcriptional control over jasmonic acid signaling. Frontiers in Plant Science, 6, 170.
Carolanv J C, Caragea D, Reardon K T, Mutti N S, Dittmer N, Pappan K, Cui F, Castaneto M, Poulain J, Dossat C, Tagu D, Reese J C, Reeck G R, Wilkinson T L, Edwards O R. 2011. Predicted effector molecules in the salivary secretome of the pea aphid (Acyrthosiphon pisum): A dual transcriptomic/proteomic approach. Journal of Proteome Research, 4, 1505–1518.
Dedryver C A, Le Ralec A, Fabre F. 2010. The conflicting relationships between aphids and men: A review of aphid damages and of their control strategies. Comptes Rendus Biologies, 333, 539–553.
Dogimont C, Bendahmane A, Chovelon V, Boissot N. 2010. Host plant resistance to aphids in cultivated crops: Genetic and molecular bases, and interactions with aphid populations. Comptes Rendus Biologies, 333, 566–573.
Edwards O, Singh K B. 2006. Resistance to insect pests: What do legumes have to offer? Euphytica, 147, 273–285.
Felton G W, Tumlinson J H. 2008. Plant-insect dialogs: complex interactions at the plant-insect interface. Current Opinion in Plant Biology, 11, 457–463.
Gao L L, Anderson J P, Klinger J P, Nair R M, Edwards O R, Singh K B. 2007. Involvement of the octadecanoid pathway in bluegreen aphid resistance in Medicago truncatula. Molecular Plant-Microbe Interactions, 20, 82–93.
Golawska S, Bogumil L, Oleszek W. 2006. Effect of low and high-saponin lines of alfalfa on pea aphid. Journal of Insect Physiology, 52, 737–743.
Grabherr M G, Haas B J, Yassour M, Levin J Z, Thompson D A, Amit I, Adiconis X, Fan L, Raychowdhury R, Zeng Q D, Chen Z H, Mauceli E, Hacohen N, Gnirke A, Rhind N, di Palma F, Birren B W, Nusbaum C, Lindblad-Toh K, Friedman N, et al. 2011. Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nature Biotechnology, 29, 644–652.
Halitschke R, Schittko U, Pohnert G, Boland W, Baldwin I T. 2001. Molecular interactions between the specialist herbivore Manduca sexta (Lepidoptera, Sphingidae) and its natural host Nicotiana attenuate. III. Fatty acid-amino acid conjugates in herbivore oral secretions are necessary and sufficient for herbivore-specific plant responses. Plant Physiology, 125, 711–717.
Hegedus D D, Rimmer S R. 2005. Sclerotinia sclerotiorum: When “to be or not to be” a pathogen? FEMS Microbiology Letters, 251, 177–184.
Hermsmeier D, Schittko U, Baldwin I T. 2001. Molecular interactions between the specialist herbivore Manduca sexta (Lepidopetra, Sphingidae) and its natural host Nicotiana attenuata. I. Large-scale changes in the accumulation of growth- and defense-related plant mRNAs. Plant Physiology, 125, 683–700.
Jones J D G, Dangl J L. 2006. The plant immune system. Nature, 444, 323–329.
Kanehisa M, Araki M, Goto S, Hattori M, Hirakawa M, Itoh M, Katayama T, Kawashima S, Okuda S, Tokimatsu T, Yamanishi Y. 2008. KEGG for linking genomes to life and the environment. Nucleic Acids Research, 36, 480–484.
Kloth K J, Wiegers G L, Busscher-Lange J, van Haarst J C, Kruijer W, Bouwmeester H J, Dicke M, Jongsma M A. 2016. AtWRKY22 promotes susceptibility to aphids and modulates salicylic acid and jasmonic acid signalling. Journal of Experimental Botany, 67, 3383–3396.
Kozjak P, Megli? V. 2012. Mutagenesis in plant breeding for disease and pest resistance. In: Mishra R, ed., Biochemistry, Genetics and Molecular Biology. Intech Press, Rijeka, Croatia. pp. 195–220.
Leon-Reyes A, Van der Does D, De Lange E S, Delker C, Wasternack C, Van Wees S C M, Ritsema T, Pieterse C M J. 2010. Salicylate-mediated suppression of jasmonate-responsive gene expression in Arabidopsis is targeted downstream of the jasmonate biosynthesis pathway. Planta, 232, 1423–1432.
Liu D, Jiang H X, Wang Z F, Cao Y, Zhang S E, Zhai G Y. 2012. The prevention and control of alfalfa aphid. Shandong Journal of Animal Husbandry Veterinary, 33, 94–96. (in Chinese)
Liu D, Xu L S, Vandemark G, Chen W D. 2016. Comparative transcriptome analysis between the fungal plant pathogens Sclerotinia sclerotiorum and S. trifoliorum using RNA sequencing. Journal of Heredity, 107, 163–172.
Lu Y, Wu K, Jiang Y, Guo Y, Desneux N. 2012. Widespread adoption of Bt cotton and insecticide decrease promotes biocontrol services. Nature, 487, 362–365.
Maleck K, Dietrich R A. 1999. Defense on multiple fronts: how do plants cope with diverse enemies? Trends in Plant Science, 4, 215–219.
Mao X, Cai T, Olyarchuk J G, Wei L. 2005. Automated genome annotation and pathway identification using the KEGG Orthology (KO) as a controlled vocabulary. Bioinformatics, 21, 3787–3793.
Martin G B, Brommonschenkel S H, Chunwongse J, Frary A, Ganal M W, Spivey R, Wu T, Earle E D, Tanksley S D. 1993. Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science, 262, 1432–1436.
Martin G B, Bogdanove A J, Sessa G. 2003. Understanding the functions of plant disease resistance proteins. Annual Review of Plant Biology, 54, 23–61.
Mithöfer A, Boland W. 2008. Recognition of herbivory associated molecular patterns. Plant Physiology, 146, 825–831.
Ng J C K, Perry K L. 2004. Transmission of plant viruses by aphid vectors. Molecular Plant Pathology, 5, 505–511.
Paiva N L. 2000. An introduction to the biosynthesis of chemicals used in plant-microbe communication. Journal of Plant Growth Regulation, 19, 131–143.
Parker J E, Coleman M J, Szabo V, Frost L N, Schmidt R, van der Biezen E A, Moores T, Dean C, Daniels M J, Jones J D. 1997. The Arabidopsis downy mildew resistance gene RPP5 shares similarity to the toll and interleukin-1 receptors with N and L6. The Plant Cell, 9, 879–894.
Prince D C, Drurey C, Zipfel C, Hogenhout S A. 2014. The leucine-rich repeat receptor-like kinase brassinosteroid insensitive 1-associated kinase 1 and the cytochrome P450 phytoalexin deficient 3 contribute to innate immunity to aphids in Arabidopsis. Plant Physiology, 164, 2207–2219.
Rossi M, Goggin F L, Milligan S B. 1998. The nematode resistance gene Mi of tomato confers resistance against the potato aphid. Proceedings of the National Academy of Sciences of the United States of America, 95, 9750–9754.
Salmeron J M, Oldroyd G E, Rommens C M, Scofield S R, Kim H S, Lavelle D T, Dahlbeck D, Staskawicz B J. 1996. Tomato Prf is a member of the leucine-rich repeat class of plant disease resistance genes and lies embedded within the Pto kinase gene cluster. Cell, 86, 123–133.
Simmonds M S J. 2003. Flavonoid-insect interactions: Recent advances in our knowledge. Phytochemistry, 64, 21–30.
Smith C M, Boyko E V. 2007. The molecular bases of plant resistance and defense responses to aphid feeding: Current status. Entomologia Experimentalis et Applicate, 122, 1–16.
Smith C M, Clement S L. 2012. Molecular bases of plant resistance to arthropods. Annual Review of Entomology, 57, 309–328.
Song W Y, Wang G L, Chen L L, Kim H S, Pi L Y, Holsten T, Gardner J, Wang B, Zhai W X, Zhu L H, Fauquet C, Ronald P. 1995. A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21. Science, 270, 1804 –1806.
Storey J D, Tibshirani R. 2003. Statistical significance for genome wide studies. Proceedings of the National Academy of Sciences of the United States of America, 100, 9440–9445.
Stotz H U, Kroymann J, Mitchell-Olds T. 1999. Plant-insect interactions. Current Opinion in Plant Biology, 2, 268–272.
Tu X B, Fan Y L, McNeill R A, Zhang Z H. 2017. Including predator presence in a refined model for assessing resistance of alfalfa cultivar to aphids. Journal of Integrative Agriculture, 18, 1–8.
Tzin V, Fernandez-Pozo N, Richter A, Schmelz E A, Schoettner M, Schäfer M, Ahern K R, Meihls L N, Kaur H, Huffaker A, Mori N, Degenhardt J, Mueller L A, Jander G. 2015. Dynamic maize responses to aphid feeding are revealed by a time series of transcriptomic and metabolomic assays. Plant Physiology, 169, 1727–1743.
Walling L L. 2000. The myriad plant responses to herbivores. Journal of Plant Growth Regulation, 19, 195–216.
Whitman D W, Ananthakrishnan T N. 2009. Phenotypic Plasticity of Insects: Mechanisms and Consequences. CRC Press, Florida.
Xu Y X. 2008. Resistance mechanism of alfalfa varieties to Therioaphis trifolli. MSc thesis, Gansu Agircultural University, China. (in Chinese)
Young M D, Wakefield M J, Smyth G K, Oshlack A. 2010. Gene ontology analysis for RNA-seq: Accounting for selection bias. Genome Biology, 11, 14.
Yu L, Liu J P, Zhuang Z X,Yang L Q, Zhang R L, Ye X M, Cheng J Q. 2007. Quantitative analysis of real-time PCR expression production by REST and 2–ΔΔCT. Journal of Tropical Medicine, 10, 956–958.
Züst T, Agrawal A A. 2016. Mechanisms and evolution of plant resistance to aphids. Nature Plants, 2, 206.

Transcriptome approach to understand the potential mechanisms of resistant and susceptible alfalfa (Medicago sativa L.) cultivars in response to aphid feeding

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics