Prevalence and characteristics of extended spectrum β-lactamaseproducing Escherichia coli from bovine mastitis cases in China

doi:10.1016/S2095-3119(17)61830-6

Journal of Integrative Agriculture ›› 2018, Vol. 17 ›› Issue (06): 1246-1251.DOI: 10.1016/S2095-3119(17)61830-6

收稿日期:2017-09-04 出版日期:2018-06-01 发布日期:2018-06-05

Prevalence and characteristics of extended spectrum β-lactamaseproducing Escherichia coli from bovine mastitis cases in China

YANG Feng, ZHANG Shi-dong, SHANG Xiao-fei, WANG Xu-rong, WANG Ling, YAN Zuo-ting, LI Hong-sheng

Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, P.R.China

Received:2017-09-04 Online:2018-06-01 Published:2018-06-05
Contact: Correspondence YANG Feng, Tel: +86-931-2115262, Fax: +86-931-2114180, E-mail: yangfeng@caas.cn; LI Hong-sheng, Tel: +86-931-2164183, Fax: +86-931-2114180, E-mail: lihsheng@sina.com
Supported by:
This work was funded by the National Key R&D Program of China (2017YFD0502200) and the Central Public-Interest Scientific Institution Basal Research Fund, China (1610322017013).

摘要/Abstract

Abstract:

The aim of the study was to investigate the prevalence and characterization of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from bovine mastitis cases in China. ChromID ESBL agar was used to confirm ESBL-producing E. coli. PCR and DNA sequencing were employed to characterize the genotype of ESBL-producers. Antimicrobial susceptibility was measured by disc diffusion. Overall, 73 of 318 E. coli isolates (22.96%) were identified as ESBL-producers. Of these ESBL-producing E. coli, the prevalence of bla_CTX-M and bla_TEM-1 was 97.26 and 71.23%, respectively. The predominant CTX-M-type ESBL was CTX-M-15 (65.75%), followed by CTX-M-14 (10.96%), CTX-M-55 (9.59%), CTX-M-64 (5.48%), CTX-M-65 (4.11%) and CTX-M-3 (1.37%). This study is the first report of CTX-M-64 and CTX-M-65 in E. coli isolated from bovine mastitis. Furthermore, 72 ESBL-producing E. coli isolates (98.63%) were found to be multidrug-resistance. This study noted high prevalence and rates of antimicrobial resistance of ESBL-producing E. coli isolates from bovine mastitis cases in China.

Key words:

extended spectrum β-lactamase , Escherichia coli , multidrug-resistance , bovine mastitis

. [J]. Journal of Integrative Agriculture, 2018, 17(06): 1246-1251.

YANG Feng, ZHANG Shi-dong, SHANG Xiao-fei, WANG Xu-rong, WANG Ling, YAN Zuo-ting, LI Hong-sheng. Prevalence and characteristics of extended spectrum β-lactamaseproducing Escherichia coli from bovine mastitis cases in China[J]. Journal of Integrative Agriculture, 2018, 17(06): 1246-1251.

参考文献

Ali T, Ur R S, Zhang L, Shahid M, Zhang S, Liu G, Gao J, Han B. 2016. ESBL-producing Escherichia coli from cows suffering mastitis in China contain clinical class 1 integrons with CTX-M linked to ISCR1. Frontiers in Microbiology, 7, doi: 10.3389/fmicb.2016.01931
Amel A, Mourad D, Claire D C, Chloé D, Alain H, Sébastien S, Eliane S, Lucie A, Catherine N. 2016. Occurence of armA and rmtB aminoglycoside resistance 16S rRNA methylases in extended-spectrum β-lactamases producing Escherichia coli in Algerian hospitals. Frontiers in Microbiology, 7, doi: 10.3389/fmicb.2016.01409
Bailey J K, Pinyon J L, Anantham S, Hall R M. 2011. Distribution of the blaTEM gene and blaTEM-containing transposons in commensal Escherichia coli. Journal of Antimicrobial Chemotherapy, 66, 745–751.
Chandramohan L, Revell P A. 2012. Prevalence and molecular characterization of extended-spectrum-β-lactamase-producing Enterobacteriaceae in a pediatric patient population. Antimicrobial Agents & Chemotherapy, 56, 4765–4770.
Chang F Y, Siu L K, Fung C P, Huang M H, Ho M. 2001. Diversity of SHV and TEM beta-lactamases in Klebsiella pneumoniae: Gene evolution in northern Taiwan and two novel beta-lactamases, SHV-25 and SHV-26. Antimicrobial Agents & Chemotherapy, 45, 2407–2413.
CLSL (Clinical and Laboratory Standards Institute). 2015. Performance Standards for Antimicrobial Susceptibility Testing: Twenty-Fifth Informational Supplement. M100–S25. CLSI, Wayne, PA.
Cressier B, Bissonnette N. 2011. Assessment of an extraction protocol to detect the major mastitis-causing pathogens in bovine milk. Journal of Dairy Science, 94, 2171–2184.
Dahmen S, Métayer V, Gay E, Madec J Y, Haenni M. 2013. Characterization of extended-spectrum beta-lactamase (ESBL)-carrying plasmids and clones of Enterobacteriaceae causing cattle mastitis in France. Veterinary Microbiology, 162, 793–799.
Freitag C, Michael G B, Kadlec K, Hassel M, Schwarz S. 2016. Detection of plasmid-borne extended-spectrum β-lactamase (ESBL) genes in Escherichia coli isolates from bovine mastitis. Veterinary Microbiology, 200, 151–156.
Geser N, Stephan R, Hächler H. 2012. Occurrence and characteristics of extended-spectrum β-lactamase (ESBL) producing Enterobacteriaceae in food producing animals, minced meat and raw milk. BMC Veterinary Research, 8, 21.
Hijazi S M, Fawzi M A, Ali F M, Abd El Galil K H. 2016. Prevalence and characterization of extended-spectrum beta-lactamases producing Enterobacteriaceae in healthy children and associated risk factors. Annals of Clinical Microbiology & Antimicrobials, 15, doi: 10.1186/s12941-016-0121-9
Hilary D, Dennis M, Jane M, Tara N, Anthony S. 2016. Special communication: Antimicrobial resistance. Journal of the American Medical Association, 316, 1193–1204.
Ho P L, Poon W W, Loke S L, Leung M S, Chow K H, Wong R C, Yip K S, Lai E L, Tsang K W. 2007. Community emergence of CTX-M type extended-spectrum beta-lactamases among urinary Escherichia coli from women. Journal of Antimicrobial Chemotherapy, 60, 140–144.
Kempf F, Slugocki C, Blum S E, Leitner G, Germon P. 2016. Genomic comparative study of bovine mastitis Escherichia coli. PLoS ONE, 11, e0147954.
Kilani H, Abbassi M S, Ferjani S, Mansouri R, Sghaier S, Ben S R, Jaouani I, Douja G, Brahim S, Hammami S, Ben Chehida N, Boubaker I B. 2015. Occurrence of blaCTX-M-1, qnrB1 and virulence genes in avian ESBL-producing Escherichia coli isolates from Tunisia. Frontiers in Cellular & Infection Microbiology, 5, doi: 10.3389/fcimb.2015.00038
Kivaria F M, Noordhuizen J P, Msami H M. 2007. Risk factors associated with the incidence rate of clinical mastitis in smallholder dairy cows in the Dar es Salaam region of Tanzania. Veterinary Journal, 173, 623–629.
Li L, Wang B, Feng S, Li J, Wu C, Wang Y, Ruan X, Zeng M. 2014. Prevalence and characteristics of extended-spectrum β-lactamase and plasmid-mediated fluoroquinolone resistance genes in Escherichia coli isolated from chickens in Anhui province, China. PLoS ONE, 9, e104356.
Liu H, Wang Y, Wang G, Xing Q, Shao L, Dong X, Sai L, Liu Y, Ma L. 2015. The prevalence of Escherichia coli strains with extended spectrum beta-lactamases isolated in China. Frontiers in Microbiology, 6, doi: 10.3389/fmicb.2015.00335
Magiorakos A P, Srinivasan A, Carey R B, Carmeli Y, Falagas M E, Giske C G, Harbarth S, Hindler J F, Kahlmeter G, Olsson-Liljequist B, Paterson D L, Rice L B, Stelling J, Struelens M J, Vatopoulos A, Weber J T, Monnet D L. 2012. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance. Clinical Microbiology & Infection, 18, 268–281.
Metzger S A, Hogan J S. 2013. Short communication: Antimicrobial susceptibility and frequency of resistance genes in Escherichia coli isolated from bovine mastitis. Journal of Dairy Science, 96, 3044–3049.
Ohnishi M, Okatani A T, Harada K, Sawada T, Marumo K, Murakami M, Sato R, Esaki H, Shimura K, Kato H, Uchida N, Takahashi T. 2013. Genetic characteristics of CTX-M-type extended-spectrum-β-lactamase (ESBL)-producing Enterobacteriaceae involved in mastitis cases on Japanese dairy farms, 2007 to 2011. Journal of Clinical Microbiology, 51, 3117–3122.
Pehlivanoglu F, Turutoglu H, Ozturk D. 2016. CTX-M-15-type extended-spectrum beta-lactamase-producing Escherichia coli as causative agent of bovine mastitis. Foodborne Pathogens & Disease, 13, 477–482.
Pitkälä A, Haveri M, Pyörälä S, Myllys V, Honkanen-Buzalski T. 2004. Bovine mastitis in Finland 2001-prevalence, distribution of bacteria, and antimicrobial resistance. Journal of Dairy Science, 87, 2433–2441.
Rao L, Lv L, Zeng Z, Chen S, He D, Chen X, Wu C, Wang Y, Yang T, Wu P, Liu Y, Liu J H. 2014. Increasing prevalence of extended-spectrum cephalosporin-resistant Escherichia coli in food animals and the diversity of CTX-M genotypes during 2003–2012. Veterinary Microbiology, 172, 534–541.
Tark D S, Moon D C, Kang H Y, Kim S R, Nam H M, Lee H S, Jung S C, Lim S K. 2017. Antimicrobial susceptibility and characterization of extended-spectrum β-lactamases in Escherichia coli isolated from bovine mastitic milk in South Korea from 2012 to 2015. Journal of Dairy Science, 100, 3463–3469.
Xu G, An W, Wang H, Zhang X. 2015. Prevalence and characteristics of extended-spectrum β-lactamase genes in Escherichia coli isolated from piglets with post-weaning diarrhea in Heilongjiang province, China. Frontiers in Microbiology, 6, doi: 10.3389/fmicb.2015.01103
Yang F, Liu L H, Li X P, Luo J Y, Zhang Z, Yan Z T, Li H S. 2016. Short communication: N-acetylcysteine-mediated modulation of antibiotic susceptibility of bovine mastitis pathogens. Journal of Dairy Science, 99, 4300–4302.
Yu T, He T, Yao H, Zhang J B, Li X N, Zhang R M, Wang G Q. 2015. Prevalence of 16S rRNA methylase gene rmtB among Escherichia coli isolated from bovine mastitis in Ningxia, China. Foodborne Pathogens & Disease, 12, 770–777.
Zhao W H, Hu Z Q. 2013. Epidemiology and genetics of CTX-M extended-spectrum β-lactamases in Gram-negative bacteria. Critical Reviews in Microbiology, 39, 79–101.

Prevalence and characteristics of extended spectrum β-lactamaseproducing Escherichia coli from bovine mastitis cases in China

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