Immunogenicity and protective efficacy of DHBV DNA vaccines expressing envelope and capsid fusion proteins in ducks delivered by attenuated Salmonella typhimurium

doi:10.1016/S2095-3119(17)61829-X

Journal of Integrative Agriculture ›› 2018, Vol. 17 ›› Issue (04): 928-939.DOI: 10.1016/S2095-3119(17)61829-X

收稿日期:2017-04-18 出版日期:2018-04-20 发布日期:2018-04-16

Immunogenicity and protective efficacy of DHBV DNA vaccines expressing envelope and capsid fusion proteins in ducks delivered by attenuated Salmonella typhimurium

LIU Si-yang^{1, 2, 3*}, JIA Ren-yong^{1, 2, 3*,} LI Qing-qing^{1, 2, 3}, FENG Dai-shen^{1, 2, 3}, SHEN Hao-yue^{1, 2, 3}, YANG Cui^{1, 2, 3}, WANG Ming-shu^{1, 2, 3}, ZHU De-kang^{1, 2, 3}, CHEN Shun^{1, 2, 3}, LIU Ma-feng^{1, 2, 3}, ZHAO Xin-xin^{1, 2, 3}, YIN Zhong-qiong³, JING Bo³, CHENG An-chun^{1, 2, 3}

¹ Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R.China
² Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China
³ Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China

Received:2017-04-18 Online:2018-04-20 Published:2018-04-16
Contact: Correspondence JIA Ren-yong, Tel/Fax: +86-28-86291176, E-mail: jiary@sicau.edu.cn; CHEN An-chun, E-mail: chenganchun@vip.163.com
About author:LIU Si-yang, E-mail: uniyaliu@163.com; * These authors contributed equally to this study.
Supported by:
This research was supported by the National Key Technology R&D Program of China (2015BAD12B05), the earmarked fund for China Agricultural Research System (CARS-43-8), the Integration and Demonstration of Key Technologies for Duck Industry in Sichuan Province, China (2014NZ0030), and the Sichuan Province Research Programs, China (2014-002).

摘要/Abstract

Abstract: Duck hepatitis B virus (DHBV) shares many basic characteristics with hepatitis B virus (HBV) and is an attractive model for vaccine development. In this study, DHBV DNA vaccines were designed to express envelope and capsid fusion proteins to enhance the breadth of immune response in ducks. Attenuated Salmonella typhimurium (SL7207) was used as a carrier and adjuvant to boost the magnitude of immune response. Based on this strategy, novel DNA vaccines (SL7207-pVAX1-LC and SL7207-pVAX1-SC) were generated. Growth kinetics, genetic stabilities and relative transcription levels of the L, S and C genes introduced by these vaccine strains were measured before inoculation to guarantee safety and efficacy. The relative transcript levels of the CD4 and CD8 T genes and the antibody levels (IgY) in ducks receiving the vaccines were higher than those in single gene delivered groups. Additionally, the copy number of covalently closed circular DNA in hepatocytes after DHBV challenge also provided evidence that our fusion vaccines could enhance the protective efficiency against DHBV infection in ducks.

Key words: fusion DNA genes , attenuated Salmonella typhimurium (SL7207) , hepatitis B virus (HBV) , duck hepatitis B virus (DHBV) , oral immunization

. [J]. Journal of Integrative Agriculture, 2018, 17(04): 928-939.

LIU Si-yang, JIA Ren-yong, LI Qing-qing, FENG Dai-shen, SHEN Hao-yue, YANG Cui, WANG Ming-shu, ZHU De-kang, CHEN Shun, LIU Ma-feng, ZHAO Xin-xin, YIN Zhong-qiong, JING Bo, CHENG An-chun . Immunogenicity and protective efficacy of DHBV DNA vaccines expressing envelope and capsid fusion proteins in ducks delivered by attenuated Salmonella typhimurium[J]. Journal of Integrative Agriculture, 2018, 17(04): 928-939.

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Immunogenicity and protective efficacy of DHBV DNA vaccines expressing envelope and capsid fusion proteins in ducks delivered by attenuated Salmonella typhimurium

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