An EMS mutant library for cucumber

doi:10.1016/S2095-3119(17)61765-9

Journal of Integrative Agriculture ›› 2018, Vol. 17 ›› Issue (07): 1612-1619.DOI: 10.1016/S2095-3119(17)61765-9

收稿日期:2017-07-27 出版日期:2018-07-04 发布日期:2018-07-05

An EMS mutant library for cucumber

CHEN Chen^{1, 2, 4}, CUI Qing-zhi², HUANG San-wen³, WANG Shen-hao³, LIU Xiao-hong², LU Xiang-yang^{1, 4, 5}, CHEN Hui-ming^{1, 2, 4}, TIAN Yun^{1, 4, 5}

¹ College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, P.R.China
² Hunan Vegetable Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, P.R.China
³ Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture/Sino-Dutch Joint Laboratory of Horticultural Genomics, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
⁴ Key Laboratory for Agricultural Biochemistry and Biotransformation of Hunan Provincial University, Changsha 410128, P.R.China
⁵ Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Changsha 410128, P.R.China

Received:2017-07-27 Online:2018-07-04 Published:2018-07-05
Contact: Correspondence CHEN Hui-ming, E-mail: huiminghm@163.com; TIAN Yun, E-mail: tianyun@hunau.edu.cn
Supported by:
This study was sponsored by the National Key R&D Program of China (2016YFD0100307), the National Natural Science Foundation of China (31471871) and the Construct Program of the Key Disciplines in Hunan Province, China.

摘要/Abstract

Abstract:

Cucumber is an important vegetable crop and a model crop for the study of sex expression in plants. However, the genomic resources and tools for functional genomics studies in cucumber are still limited. In this paper, we conducted ethyl methyl sulfone (EMS) mutagenesis in the northern China ecotype cucumber inbred line 406 to construct a mutant library. We optimized the conditions of EMS mutagenesis on inbred line 406 which included treatment of seeds at 1.5% EMS for 12 h. We obtained a number of mutant lines showing inheritable morphological changes in plant architecture, leaves, floral organs, fruits and other traits through M1, M2 and M3 generations. The F2 segregating populations were constructed and analyzed.We found that a short fruit mutant and a yellow-green fruit peel mutant were both under the control of a single recessive gene, respectively. These results provide valuable germplasm resources for the improvement of cucumber genetics and functional genomic research.

Key words:

cucumber , EMS, mutant library , traits

. [J]. Journal of Integrative Agriculture, 2018, 17(07): 1612-1619.

CHEN Chen, CUI Qing-zhi, HUANG San-wen, WANG Shen-hao, LIU Xiao-hong, LU Xiang-yang, CHEN Hui-ming, TIAN Yun. An EMS mutant library for cucumber[J]. Journal of Integrative Agriculture, 2018, 17(07): 1612-1619.

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An EMS mutant library for cucumber

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