The positive function of selenium supplementation on reducing nitrate accumulation in hydroponic lettuce (Lactuca sativa L.)

doi:10.1016/S2095-3119(17)61759-3

摘要/Abstract

Abstract: High nitrate (NO₃⁻) in vegetables, especially in leaf vegetables poses threaten to human health. Selenium (Se) is an important element for maintaining human health, and exogenous Se application during vegetable and crop production is an effective way to prevent Se deficiency in human bodies. Exogenous Se shows positive function on plant growth and nutrition uptake under abiotic and/or biotic stresses. However, the influence of exogenous Se on NO₃⁻ accumulation in hydroponic vegetables is still not clear. In the present study, hydroponic lettuce plants were subjected to six different concentrations (0, 0.1, 0.5, 5, 10 and 50 µmol L^–1) of Se as Na₂SeO₃. The effects of Se on NO₃⁻ content, plant growth, and photosynthetic capacity of lettuce (Lactuca sativa L.) were investigated. The results showed that exogenous Se positively decreased NO₃⁻ content and this effect was concentration-dependent. The lowest NO₃⁻ content was obtained under 0.5 µmol L^–1 Se treatment. The application of Se enhanced photosynthetic capacity by increasing the photosynthesis rate (P_n), stomatal conductance (Cs) and the transpiration efficiency (T_r) of lettuce. The transportation and assimilation of NO₃⁻ and activities of nitrogen metabolism enzymes in lettuce were also analysed. The NO₃⁻ efflux in the lettuce roots was markedly increased, but the efflux of NO₃⁻ from the root to the shoot was decreased after treated with exogenous Se. Moreover, Se application stimulated NO₃⁻ assimilation by enhancing nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS) and glutamate synthase enzyme (GOGAT) activities. These results provide direct evidence that exogenous Se shows positive function on decreasing NO₃⁻ accumulation via regulating the transport and enhancing activities of nitrogen metabolism enzyme in lettuce. We suggested that 0.5 µmol L^–1 Se can be used to reduce NO₃⁻ content and increase hydroponic lettuce yield.

Key words: selenium , NO₃^&minus, , nitrogen metabolism enzyme , SIET , photosynthetic performance , lettuce

. [J]. Journal of Integrative Agriculture, 2018, 17(04): 837-846.

LEI Bo, BIAN Zhong-hua, YANG Qi-chang, WANG Jun, CHENG Rui-feng, LI Kun, LIU Wen-ke, ZHANG Yi, FANG Hui, TONG Yun-xin. The positive function of selenium supplementation on reducing nitrate accumulation in hydroponic lettuce (Lactuca sativa L.)[J]. Journal of Integrative Agriculture, 2018, 17(04): 837-846.

参考文献

Aslam M, Harbit K B, Huffaker R C. 1990. Comparative effects of selenium and selenate on nitrate assimilation in barley seedlings. Plant, Cell and Environment, 13, 773–782.

Barneix A J. 2007. Physiology and biochemistry of source-regulated protein accumulation in the wheat grain. Journal of Plant Physiology, 164, 581–590.

Bian Z H, Cheng R F, Yang Q C, Wang J, Lu C G. 2016 Continuous light from red, blue, and green light-emitting diodes reduces nitrate content and enhances phytochemical concentrations and antioxidant capacity in lettuce. Journal of American Society for Horticultural Science, 141, 186–195.

Bian Z H, Yang Q C, Liu W K. 2015. Effects of light quality on the accumulation of phytochemicals in vegetables produced in controlled environments: A review. Journal of the Science of Food and Agriculture, 95, 869–877.

Canovas F M, Canton F R, Gallardo F, Garcia-Gutierrez A, de Vicente A. 1991. Accumulation of glutamine synthetase during early development of maritime pine pinus-pinaster seedlings. Planta, 185, 372–378.

Cartes P, Gianfreda L, Mora M L. 2005. Uptake of selenium and its antioxidant activity in ryegrass when applied as selenate and selenium forms. Plant and Soil, 276, 359–367.

Cataldo D A, Haroon M, Schrader L E, Youngs V L. 1975. Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid. Communications in Soil and Science Plant Analysis, 6, 71–80.

Champigny M L. 1995. Integration of photosynthetic carbon and nitrogen metabolism in higher plants. Photosynthese Research, 46, 117–127.

Chen F L, Cullimore J V. 1988. Two isoenzymes of NADH-dependent glutamate synthase in root nodules of Phaseolus vulgaris L. Plant Physiology, 88, 1411–1417.

Cometti N N, Martins M Q, Bremenkamp C A, Nunes J A. 2011. Nitrate concentration in lettuce leaves depending on photosynthetic photon flux and nitrate concentration in the nutrient solution. Horticultura Brasileira, 29, 548–553.

Diwadkar-Navsariwala V, Prins G S, Swanson S M, Birch L A, Ray V H, Hedayat S. 2006. Selenoprotein deficiency accelerates prostate carcinogenesis in a transgenic model. Proceedings of the National Academy Sciences of the United States of America, 103, 8179–8184.

van Eysinga J P N L R, van der Meijs M Q. 1985. Effect of nitrogen nutrition and global radiation on yield and nitrate and nitrate content of lettuce grown under glass. Communications in Soil and Science Plant Analysis, 16, 1293–1300.

Fan S C, Lin C S, Hsu P, Lin S, Tsay Y F. 2009. The Arabidopsis nitrate transporter NRT1.7, expressed in phloem, is phloem, is responsible for source-to-sink remobilization of nitrate. The Plant Cell, 21, 2750–2761.

Feng R, Wei C, Tu S. 2013. The roles of selenium in protecting plants against abiotic stresses. Environmental and Experimental Botany, 87, 58–68.

Forde B G, Clarkson D T. 1999. Nitrate and ammonium nutrition of plants: Physiological and molecular perspectives. Advances in Botanical Research, 30, 1–90.

Forde B G, Lea P J. 2007. Glutamate in plants: Metabolism, regulation, and signaling. Journal of Experimental Botany, 58, 2339–2358.

Garnett T P, Shabala S N, Smethurst P J, Newman I A. 2001. Simultaneous measurement of ammonium, nitrate and proton fluxes along the length of eucalypt roots. Plant and Soil, 236, 55–62.

Golob A, Gad?o D, Stibilj V, Djiki? M, Gavri? T, Kreft I, Germ M. 2016. Sulphur interferes with selenium accumulation in Tartary buckwheat plants. Plant Physiology and Biochemistry, 108, 32–36.

Hageman R H, Reed A J. 1980. Nitrate reductase from higher plants. Methods in Enzymology, 69, 270–280.

Hartikainen H. 2005. Biogeochemistry of selenium and its impact on food chain quality and human health. Journal of Trace Elements in Medicine and Biology, 18, 309-318.

Hawkesford M J, Zhao F J. 2007. Strategies for increasing the selenium content of wheat. Journal of Cereal Science, 46, 282–292.

Hawkins B J, Boukcim H, Plassard C A. 2008. A comparison of ammonium, nitrate and proton net fluxes along seedling roots of Douglas-fir and lodgepole pine grown and measured with different inorganic nitrogen sources. Plant, Cell and Environment, 31, 278–-287.

Hawrylak-Nowak B. 2008. Changes in anthocyanin content as indicator of maize sensitivity to selenium. Journal of Plant Nutrition, 31, 1232–1242.

Hoagland D R, Arnon D I. 1950. The water-culture method for growing plants without soil. Circular California Agricultural Experiment Station, 347, 357–359.

Ingemarsson B, Oscarson P, Ugglas M af, Larsson C M. 1987. Nitrogen utilization in Lemna. II. Studies of nitrate uptake using 13NO3-. Plant Physiology, 85, 860–864.

Jozwiak W, Mleczek M, Politycka B. 2016. The effect of exogenous selemium on the growth and photosynthetic pigments of cucumber seedlings. Fresenius Environmental Bulletion, 25, 142–152.

Kochian L V, Shaff J E, Kuehtreiber W M, Jaffe L F, Lucas W J. 1992. Use of an extracellular, ion-selective, vibrating microelectrode system for the quantification of potassium, proton, and calcium positive fluxes in maize roots and maize suspension cells. Planta, 188, 601–610.

Li J Y, Fu Y L, Pike S M, Bao J, Tian W, Zhang Y, Chen C Z, Zhang Y, Li H M, Huang J, Li L G, Schroeder J I, Gassmann W, Gonga J M. 2010. The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal from the xylem sap and mediates cadmium tolerance. The Plant Cell, 22, 1633–1646.

Liu D D, Li H, Wang Y Z, Ying Z Z, Bian Z W, Zhu W L, Liu W, Yang L F, Jiang, D H. 2017. How exogenous selenium affects anthocyanin accumulation and biosynthesis-related gene expression in purple lettuce. Polish Journal of Environmental Studies, 26, 717–722.

Mendez J M, Vega J M. 1981. Purification and molecular properties of nitrite reductase from Anabaena sp.7119. Physiologia Plantarum, 5, 7–14.

Messaoudi I E, Heni J, Hammouda F, Said K, Kerkeni A. 2009. Protective effects of selenium, zinc, or their combination on cadmium-induced oxidative stress in rat kidney. Biological Trace Element Research, 130, 152–161.

Miflin B J, Lea P J. 1976. The pathway of nitrogen assimilation in plants. Phytochemistry, 15, 873–885.

Nowak J, Kaklewski K, Ligocki M. 2004. Influence of selenium on oxidoreductive enzymes activity in soil and in plants. Soil Biology and Biochemistry, 36, 1553–1558.

Papp L V, Lu J, Holmgren A, Khanna K K. 2007. From selenium to selenoproteins: Synthesis, identity, and their role in human health. Antioxidants & Redox Signaling, 9, 775–806.

Pedrero Z, Madrid Y, Camara C. 2006. Selenium species bloaccessibility in enriched radish (Raphanus sativus): A potential dietary source of selenium. Journal of Agricultural and Food Chemistry, 54, 2412–2417.

Peng A, Xu Y, Liu J H, Wang Z J. 2000. Study on the dose-effect relationship of selenium with the growth of wheat. Biological Trace Element Research, 76, 175–181.

Prasad S, Chetty A A. 2008. Nitrate-N determination in leafy vegetables: Study of the effects of cooking and freezing. Food Chemistry, 106, 772–780.

Rani N, Dhillon K S, Dhillon S K. 2005. Critical levels of selenium in different crops grown in an alkaline silty loam soil treated with selenium-Se. Plant Soil, 277, 367–374.

Rios J J, Blasco B, Rosales M A, Sanchez-Rodriguez E, Leyva R, Cervilla L M, Romero L, Ruiz J M. 2010. Response of nitrogen metabolism in lettuce plants subjected to different doses and forms of selenium. Journal of American Society for Horticultural Science, 90, 1914–1919.

Ruiz J M, Baghour M, Bretones G, Belakbir A, Romero L. 1998. Nitrogen metabolism in tobacco plants (Nicotiana tabacum L.): Role of boron as a possible regulatory factor. International Journal of Plant Sciences, 159, 121–126.

Ruiz J M, Castilla N, Romero L. 2000. Nitrogen metabolism in pepper plants applied with different bioregulators. Journal of Agricultural and Food Chemistry, 48, 2925–2929.

Ruiz J M, Rivero R M, Garcia P C, Baghour M, Romero L. 1999. Role of CaCl2 in nitrate assimilation in leaves and roots of tobacco plants (Nicotiana tabacum L). Plant Science, 141, 107–115.

Ruiz J M, Rivero R M, Romero L. 2007. Comparative effect of Al, Se, and Mo toxicity on NO3- assimilation in sunflower (Helianthus annuus L.) plants. Journal of Environmental Management, 83, 207–212.

Santamaria P. 2006. Nitrate in vegetables: Toxicity, content, intake and EC regulation. Journal of the Science of Food and Agriculture, 86, 10–17.

Schwarz K, Foltz C M. 1957. Selenium as an integral part of Factor 3 against dietary degeneration. Journal of the American Chemical Society, 79, 3292–3293.

Shekari L, Kamelmanesh M M, Mozafariyan M, Hasanuzzaman M, Sadeghi F. 2017. Role of selenium in mitigation of cadmium toxicity in pepper grown in hydroponic condition. Journal of Plant Nutrition, 40, 761–772.

Simojoki A, Xue T L, Lukkari K, Pennanen A, Hartikainen H. 2003. Allocation of added selenium in lettuce and its impact on roots. Agricultrual and Food Science in Finland, 12, 155–164.

Sivasankar S, Oaks A. 1996. Nitrate assimilation in higher plants: The effect of metabolites and light. Plant Physiology and Biochemistry, 34, 609–620.

Stitt M. 1999. Nitrate regulation of metabolism and growth. Current Opinion in Plant Biology, 2, 178–186.

Sun H Y, Wang X Y, Dai H X, Zhang G P, Wu F B. 2013. Effect of exogenous glutathione and selenium on cadmium-induced changes in cadmium and mineral concentrations and antioxidative metabolism in maize seedlings. Asian Journal of Chemistry, 25, 2970–2975.

Sun J, Chen S, Dai S, Wang R, Li N, Shen X, Zhou X Y, Lu C F, Zheng X J, Hu Z M. 2009. NaCl-induced alternations of cellular and tissue ion fluxes in roots of salt-resistant and salt-sensitive poplar species. Plant Physiology, 149, 1141–1153.

Takeda T, Kondo K, Ueda K, Iida A. 2016. Antioxidant responses of selenium-enriched broccoli sprout (Brassica oleracea) to paraquat exposure. Biomedical Research on Trace Elements, 27, 8–14.

Temple S J, Vance C P, Gantt J S. 1998. Glutamate synthase and nitrogen assimilation. Trends in Plant Science, 3, 51–56.

Tischner R. 2000. Nitrate uptake and reduction in higher and lower plants. Plant Cell and Environment, 23, 1005–1024.

Turakainen M, Hartikainen H, Seppanen M M. 2004. Effects of selenium treatments on potato (Solanum tuberosum L.) growth and concentrations of soluble sugars and starch. Journal of Agricultural and Food Chemistry, 52, 5378–5382.

UKMAFF (UK Ministry of Agriculture, Fisheries and Food). 1997/1998. Monitoring programmes for nitrate in lettuce and spinach. Food Surveillance Information Sheet, 154, 19.

Wang Y Y, Tsay Y F. 2011. Arabidopsis nitrate transporter NRT1.9 is important in phloem nitrate transport. The Plant Cell, 23, 1945–1957.

Wright M J, Davison K L. 1964. Nitrate accumulation in crops and nitrate poisoning in animals. Advances in Agronomy, 16, 197–247.

Xia Y, Liu S, Li H, Chen X. 2012. Effects of selenium on physiological characteristics, selenium content and quality of garlic. Plant Nutrition and Fertilizer Science, 18, 733–741.

Yan K, Chen P, Shao H B, Zhao, S J, Zhang L H, Zhang LW, Xu G, SundayJ N. 2012. Photosynthetic characterization of Jerusalem artichoke during leaf expansion. Acta Physiologiae Plantarum, 34, 353–360.

Yang D P, Guo Z H, Green I D, Xie D. 2016. Effect of cadmium accumulation on mineral nutrient levels in vegetable crops: Potential implications for human health. Environmental Science and Pollution Research, 23, 19744–19753.

Yue X, Tong S, Liping Y. 2006. Application of non-invasive microsensing system to simultaneously measure both H+ and O2 fluxes around the pollen tube. Journal of Integrative Plant Biology, 48, 823–831.

Zhang M, Tang S, Huang X, Zhang F, Pang Y, Huang Q Y, Yi Q. 2014. Selenium uptake, 450 dynamic changes in selenium content and its influence on photosynthesis and chlorophyll fluorescence in rice (Oryza sativa L.). Environmental and Experimental Botany, 107, 39–45.