Structure and expression analysis of the sucrose synthase gene family in apple

doi:10.1016/S2095-3119(17)61755-6

Journal of Integrative Agriculture ›› 2018, Vol. 17 ›› Issue (04): 847-856.DOI: 10.1016/S2095-3119(17)61755-6

收稿日期:2017-03-13 出版日期:2018-04-20 发布日期:2018-04-16

Structure and expression analysis of the sucrose synthase gene family in apple

TONG Xiao-lei1^*, WANG Zheng-yang^1*, MA Bai-quan¹, ZHANG Chun-xia², ZHU Ling-cheng¹, MA Feng-wang¹, LI Ming-jun¹

¹ State Key Laboratory of Crop Stress Biology for Arid Areas (SKLCSBAA)/College of Horticulture, Northwest A&F University, Yangling 712100, P.R.China
² College of Forestry, Northwest A&F University, Yangling 712100, P.R.China

Received:2017-03-13 Online:2018-04-20 Published:2018-04-16
Contact: Correspondence LI Ming-jun, Tel/Fax: +86-29-87082613, E-mail: limingjun@nwafu.edu.cn
About author:TONG Xiao-lei, E-mail: xiaoleitong322@163.com; WANG Zheng-yang, E-mail: 691319339@qq.com; * These authors contributed equally to this study.
Supported by:
This work was supported in part by the National Natural Science Foundation of China (31372038) and the Natural Basic Research Plan in Shaanxi Province of China (2015JQ3082).

摘要/Abstract

Abstract: Sucrose synthases (SUS) are a family of enzymes that play pivotal roles in carbon partitioning, sink strength and plant development. A total of 11 SUS genes have been identified in the genome of Malus domestica (MdSUSs), and phylogenetic analysis revealed that the MdSUS genes were divided into three groups, named as SUS I, SUS II and SUS III, respectively. The SUS I and SUS III groups included four homologs each, whereas the SUS II group contained three homologs. SUS genes in the same group showed similar structural characteristics, such as exon number, size and length distribution. After assessing four different tissues, MdSUS1s and MdSUS2.1 showed the highest expression in fruit, whereas MdSUS2.2/2.3 and MdSUS3s exhibit the highest expression in shoot tips. Most MdSUSs showed decreased expression during fruit development, similar to SUS enzyme activity, but both MdSUS2.1 and MdSUS1.4 displayed opposite expression profiles. These results suggest that different MdSUS genes might play distinct roles in the sink-source sugar cycle and sugar utilization in apple sink tissues.

Key words: apple , sucrose synthase , phylogenetic analysis , gene expression , enzyme activity

. [J]. Journal of Integrative Agriculture, 2018, 17(04): 847-856.

TONG Xiao-lei, WANG Zheng-yang, MA Bai-quan, ZHANG Chun-xia, ZHU Ling-cheng, MA Feng-wang, LI Ming-jun. Structure and expression analysis of the sucrose synthase gene family in apple[J]. Journal of Integrative Agriculture, 2018, 17(04): 847-856.

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Structure and expression analysis of the sucrose synthase gene family in apple

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