Host status of Brachypodium distachyon to the cereal cyst nematode

doi:10.1016/S2095-3119(17)61745-3

Journal of Integrative Agriculture ›› 2018, Vol. 17 ›› Issue (2): 381-388.DOI: 10.1016/S2095-3119(17)61745-3

收稿日期:2017-05-18 出版日期:2018-02-20 发布日期:2018-02-01

Host status of Brachypodium distachyon to the cereal cyst nematode

CHEN Chang-long^{1, 2}, LIU Shu-sen^{1, 3}, LIU Qian¹, NIU Jun-hai⁴, LIU Pei¹, ZHAO Jian-long¹, LIU Zhi-yong^{5, 6}, LI Hong-jie², JIAN Heng¹

¹ Department of Plant Pathology, China Agricultural University, Beijing 100193, P.R.China
² National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
³ Plant Protection Institute, Hebei Academy of Agricultural and Forestry Sciences/IPM Center of Hebei Province/Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture, Baoding 07100, P.R.China
⁴ Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, P.R.China
⁵ Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, P.R.China
⁶ Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, P.R.China

Received:2017-05-18 Online:2018-02-20 Published:2018-02-01
Contact: Correspondence JIAN Heng, Tel/Fax: +86-10-62731102, E-mail: hengjian@cau.edu.cn
About author:CHEN Chang-long, E-mail: chenchanglong319@126.com
Supported by:
We are grateful to Profs. Chen Shulong and Li Xiuhua (Plant Protection Institute, Hebei Academy of Agricultural and Forestry Sciences, Baoding, China), Li Honglian (Henan Agricultural University, Zhengzhou, China), and Li Hongmei (Nanjing Agricultural University, Nanjing, China) for helping with collections or for providing different geographical populations of Heterodera avenae. This study was funded by the National Key Basic Research Program of China (2013CB127501), the Special Fund for Agro-scientific Research in the Public Interest in China (201503114 and 200903040) and the National Key Research and Development Program of China (SQ2017ZY060063-01).

摘要/Abstract

Abstract: Cereal cyst nematode (Heterodera avenae, CCN) distributes worldwide and has caused severe damage to cereal crops, and a model host will greatly aid in the study of this nematode. In this research, we assessed the sensitivity of 25 inbred lines of Brachypodium distachyon to H. avenae from Beijing, China. All lines of B. distachyon were infested by second-stage juveniles (J2s) of H. avenae from Daxing District of Beijing population, but only 13 inbred lines reproduced 0.2–3 cysts/plant, showing resistance. The entire root system of the infested B. distachyon appeared smaller and the fibrous roots were shorter and less numerous. We found that a dose of 1 000 J2s of H. avenae was sufficient for nematode infestation. We showed that Koz-1 of B. distachyon could reproduce more cysts than TR2A line. Line Koz-1 also supported the complete life cycles of 5 CCN geographical populations belonging to the Ha1 or Ha3 pathotype group. Our results suggest that B. distachyon is a host for CCN.

Key words: susceptibility identification , Brachypodium distachyon , cereal cyst nematode , Heterodera avenae , host

. [J]. Journal of Integrative Agriculture, 2018, 17(2): 381-388.

CHEN Chang-long, LIU Shu-sen, LIU Qian, NIU Jun-hai, LIU Pei, ZHAO Jian-long, LIU Zhi-yong, LI Hong-jie, JIAN Heng . Host status of Brachypodium distachyon to the cereal cyst nematode[J]. Journal of Integrative Agriculture, 2018, 17(2): 381-388.

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Host status of Brachypodium distachyon to the cereal cyst nematode

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