Identification and expression patterns of alcohol dehydrogenase genes involving in ester volatile biosynthesis in pear fruit

doi:10.1016/S2095-3119(17)61686-1

Journal of Integrative Agriculture ›› 2017, Vol. 16 ›› Issue (08): 1742-1750.DOI: 10.1016/S2095-3119(17)61686-1

收稿日期:2016-11-29 出版日期:2017-08-20 发布日期:2017-08-02

Identification and expression patterns of alcohol dehydrogenase genes involving in ester volatile biosynthesis in pear fruit

QIN Gai-hua^{1, 2*}, QI Xiao-xiao^{2, 3*}, QI Yong-jie^{1, 2}, GAO Zheng-hui^{1, 2}, YI Xing-kai^{1, 2}, PAN Hai-fa^{1, 2}, XU Yi-liu^{1, 2}

¹ Horticultural Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, P.R.China
² Anhui Key Laboratory of Genetic Improvement and Ecophysiology of Horticultural Crop, Hefei 230031, P.R.China
³ College of Agriculture, Medicine and Health, Anhui Radio and Television University, Hefei 230022, P.R.China

Received:2016-11-29 Online:2017-08-20 Published:2017-08-02
Contact: Correspondence XU Yi-liu, Tel: +86-551-62160121, Fax: +86-551-65160937, E-mail: yiliuxu@163.com
About author:QIN Gai-hua, E-mail: 59672211@qq.com;
Supported by:
This work was financially supported by the National Natural Science Foundation of China (31301739) and the Key Technologies R&D Program of China during the 12th Five-year Plan Period (2013BAD02B01-4).

摘要/Abstract

Abstract: Alcohol dehydrogenase (ADH) catalyzes the interconversion of aldehydes and their corresponding alcohols, and is a key enzyme in volatile ester biosynthesis. However, little is known regarding ADH and ADH encoding genes (ADHs) in pear. We identified 8 ADHs in the pear’s genome (PbrADHs) by multiple sequences alignment. The PbrADHs were highly homologous in their coding regions, while were diversiform in structure. 9 introns were predicted in PbrADH₃–PbrADH₈, while 8 introns, generated through exon fusion and intron loss, were predicted in PbrADH₁ and PbrADH₂. To study the genetic regulation underlying aroma biogenesis in pear fruit, we determined the PbrADH transcripts, ADH activities and volatile contents of fruits during ripening stage for Nanguoli and Dangshansuli, two cultivars having different aroma characteristics. ADH activity was strongly associated with the transcription of ADH6 in the two cultivars during fruit ripening stage. The higher ester content paralleling to a higher ADH activity was detected in Nanguoli than in Dangshansuli, so it is induced that the lower ester content in Dangshansuli fruit may be the result of weak ADH activity. The present study revealed that total ADH activity and volatile ester production correlated with increased PbrADH₆ transcript levels. PbrADH6 may contribute to ADH activity catalyzing aldehyde reduction and ester formation in pear fruit.

Key words: alcohol dehydrogenase , ester volatile , gene expression , pear

. [J]. Journal of Integrative Agriculture, 2017, 16(08): 1742-1750.

QIN Gai-hua, QI Xiao-xiao, QI Yong-jie, GAO Zheng-hui, YI Xing-kai, PAN Hai-fa, XU Yi-liu. Identification and expression patterns of alcohol dehydrogenase genes involving in ester volatile biosynthesis in pear fruit[J]. Journal of Integrative Agriculture, 2017, 16(08): 1742-1750.

参考文献

Beekwilder J, Alvarez-Huerta M, Neef E, Verstappen F W A, Bouwmeester H J, Aharoni A. 2004. Functional characterization of enzymes forming volatile volatile ester from strawberry and banana. Plant Physiology, 135, 1865–1878.

Boom C E M V D, Beek T AV, Posthumus M A, Groot A D, Dicke M. 2004. Qualitative and quantitative variation among volatile profiles induced by Tetranychus urticae feeding on plants from various families. Journal of Chemical Ecology, 30, 69–89.

Bradford M M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248–254.

Cheng F F, Hu T, An Y, Huang J Q, Xu Y W. 2013. Purification and enzymatic characterization of alcohol dehydrogenase from Arabidopsis thaliana. Protein Expression and Purification, 90, 74–77.

Cumplido-Laso G, Medina-Puche L, Moyano E, Hoffmann T, Sinz Q, Ring L, Studart-Wittkowski C, Caballero J L, Schwab W, Muñoz-Blanco J. 2012. The fruit ripening-related gene FaAAT2 encodes an acyl transferase involved in strawberry aroma biogenesis. Journal of Experimental Botany, 63, 4275–4290.

Defilippi B G, Kader A A, Dandekar A M. 2005. Apple aroma: Alcohol acyltransferase, a rate limiting step for ester biosynthesis, is regulated by ethylene. Plant Science, 168, 1199–1210.

Defilippi B G, Manriquez D, Luengwilai K, González-Agüero M. 2009. Aroma volatiles: Biosynthesis and mechanisms of modulation during fruit ripening. Advances in Botanical Research, 50, 1–37.

Dixon J, Hewett E. 2000. Factors affecting apple aroma/flavour volatile concentration: A review. New Zealand Journal of Crop and Horticultural Science, 28, 155–173.

Echeverria G, Graell J, Lopez M L, Lara I. 2004. Volatile production, quality, and aroma-related enzyme activities during maturation of ‘‘Fuji’’ apples. Postharvest Biology and Technology, 31, 217–227.

Fellman J, Miller T, Mattinson D, Mattheis J. 2000. Factors that influence biosynthesis of volatile flavour compounds in apple fruits. HortScience, 35, 1026–1033.

Freilich S, Lev S, Gonda I, Reuveni E, Portnoy V, Oren E, Lohse M, Galpaz N, Bar E, Tzuri G. 2015. Systems approach for exploring the intricate associations between sweetness, color and aroma in melon fruits. BMC Plant Biology, 15, 71.

Gonzalez-Aguero M, Troncoso S, Gudenschwager O, Campos-Vargas R, Moya-Leon M A, Defilippi B G. 2009. Differential expression levels of aroma-related genes during ripening of apricot (Prunus armeniaca L.). Plant Physiology and Biochemistry, 47, 435–440.

Hadi M A M E, Zhang F J, Wu F F, Zhou C H, Tao J. 2013. Advances in fruit aroma volatile research. Molecules, 18, 8200–8229.

Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods, 25, 402–408.

Longhurst T, Lee E, Hinde R, Brady C, Speirs J. 1994. Structure of the tomato Adh2 gene and Adh2 pseudogenes, and a study of Adh2 gene expression in fruit. Plant Molecular Biology, 26, 1073–1084.

Manríquez D, El-Sharkawy I, Flores F B, El-Yahyaoui F, Regad F, Bouzayen M, Latche A, Pech J C. 2006. Two highly divergent alcohol dehydrogenases of melon exhibit fruit ripening-specific expression and distinct biochemical characteristics. Plant Molecular Biology, 61, 75–685.

Moummou H, Tonfack L B, Chervin C, Benichou M, Youmbi E, Ginies C, Latché A, Pech J C, Rest V D B. 2012. Functional characterization of SlscADH1, a fruit-ripening-associated short-chain alcohol dehydrogenase of tomato. Journal of Plant Physiology, 169, 1435–1444.

Park K C, Kwon S J, Kim N S. 2010. Intron loss mediated structural dynamics and functional differentiation of the polygalacturonase gene family in land plants. Genes & Genomics, 32, 570–577.

Paterson A, Kassim A, McCallum S, Woodhead M, Smith K, Zait D, Graham J. 2013. Environmental and seasonal influences on red raspberry flavour volatiles and identification of quantitative trait loci (QTL) and candidate genes. Theoretical and Applied Genetics, 126, 33–48.

Perry D J, Furnier G R. 1996. Pinus banksiana has at least seven expressed alcohol dehydrogenase genes in two linked groups. Proceedings of the National Academy of Sciences of the United States of America, 93, 13020–13023.

Qin G H, Tao S T, Cao Y F, Wu J Y, Zhang H P, Huang W J, Zhang S L. 2012. Evaluation of the volatile profile of 33 Pyrus ussuriensis cultivars by HS-SPME with GC–MS. Food Chemistry, 134, 2367–2382.

Qin G H, Tao S T, Zhang H P, Huang W J, Wu J Y, Xu Y L, Zhang S L. 2014. Evolution of the aroma volatiles of pear fruits supplemented with fatty acid metabolic precursors. Molecules, 19, 20183–20196.

Singh R K, Sane V A, Misra A, Ali S A, Nath P. 2010. Differential expression of the mango alcohol dehydrogenase gene family during ripening. Phytochemistry, 71, 1485–1494.

Song J, Du L, Li L, Palmer L C, Fomey C F, Fillmore S, Zhang Z Q, Li X H. 2015. Targeted quantitative proteomic investigation employing multiple reaction monitoring on quantitative changes in proteins that regulate volatile biosynthesis of strawberry fruit at different ripening stages. Journal of Proteomics, 126, 288–295.

Speirs J, Correll R, Cain P. 2002. Relationship between ADH activity, ripeness and softness in six tomato cultivars. Scientia Horticulturae, 93, 137–142.

Strommer J. 2011.The plant ADH gene family. The Plant Journal, 66, 128–142.

Tesnière C, Verriès C. 2000. Molecular cloning and expression of cDNAs encoding alcohol dehydrogenases from Vitis vinifera L. during berry development. Plant Science, 157, 77–88.

Tonfack L B, Moummou H, Latche A, Youmbi E, Benichou M, Pech J C, Rest B V D. 2011. The plant SDR superfamily: Involvement in primary and secondary metabolism. Current Opinion in Plant Biology, 12, 41–53.

Wu J, Wang Z W, Shi Z B, Zhang S, Ming R, Zhu S L, Khan M A, Tao S T, Korban S S, Wang H, Chen N J, Nishio T, Xu X, Cong L, Qi K J, Huang X S, Wang Y T, Zhao X, Deng C, Gou C Y, et al. 2013. The genome of the pear (Pyrus bretschneideri Rehd.). Genome Research, 23, 396–408.

Xu G X, Guo C, Shan, H Y, Kong H Z. 2012. Divergence of duplicate genes in exon-intron structure. Proceedings of the National Academy of Sciences of the United States of America, 109, 1187–1192.

Xu X Q, Liu B, Zhu B Q, Lan Y B, Gao Y, Wang D, Reeves M J, Duan C Q. 2015. Differences in volatile profiles of Cabernet Sauvignon grapes grown in two distinct regions of China and their responses to weather conditions. Plant Physiology and Biochemistry, 89, 123–133.

Yang X, Song J, Du L, Forney C, Campbell-Palmer L, Fillmore S, Wismer P, Zhang Z. 2016. Ethylene and 1-MCP regulate major volatile biosynthetic pathways in apple fruit. Food Chemistry, 194, 325–336.

Zhang B, Shen J Y, Wei W W, Xi WP, Xu C J, Ferguson I, Chen K. 2010. Expression of genes associated with aroma formation derived from the fatty acid pathway during peach fruit ripening. Journal of Agricultural & Food Chemistry, 58, 6157–6165.

Zhang D,Yu B, Bai J, Qian M, Shu Q, Su J, Teng Y. 2012. Effects of high temeperates on UV-B visibile irradiation induced postharvest anthocyanin accumulation in ‘Yuhongli No 1’ (Pyrus pyifolia Nakai) pear. Scientia Horticulturae, 134, 53–59.

Identification and expression patterns of alcohol dehydrogenase genes involving in ester volatile biosynthesis in pear fruit

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