Development of elite restoring lines by integrating blast resistance and low amylose content using MAS

doi:10.1016/S2095-3119(17)61684-8

摘要/Abstract

Abstract: Blast resistance and grain quality are major problems in hybrid rice production in China. In this study, two resistance (R) genes, Pi46 and Pita, along with the gene Wx^b, which mainly affects rice endosperm amylose content (AC), were introgressed into an elite indica restoring line, R8166, which has little blast resistance and poor grain quality through marker-assisted selection (MAS). Eight improved lines were found to have recurrent genome recovery ratios ranging from 88.68 to 96.23%. Two improved lines, R163 and R167, were selected for subsequent studies. R167, which has the highest recovery ratio (96.23%), showed no significant differences in multiple agronomic traits. In contrast, R163 with the lowest recovery ratio (88.68%) exhibited significant differences in heading date and yield per plant compared with the recurrent parent. At two developmental stages, R163 and R167 had greatly enhanced resistance to blast over the recurrent parent. Similar trends were also observed for agronomic traits and blast resistance in R163- and R167-derived hybrids when compared with the counterparts from R8166. In addition, R163, R167, and their derived hybrids significantly improved the grain quality traits, including amylose content (AC), gel consistency (GC), chalky grain rate (CGR), and degree of endosperm chalkiness (DEC). It confirmed the success of efficiently developing elite restoring lines using MAS in this study.

Key words: rice , restoring line , blast resistance , grain quality , MAS

. [J]. Journal of Integrative Agriculture, 2018, 17(01): 16-27.

XIAO Wu-ming, PENG Xin, LUO Li-xin, LIANG Ke-qin, WANG Jia-feng, HUANG Ming, LIU Yong-zhu, GUO Tao, LUO Wen-long, YANG Qi-yun, ZHU Xiao-yuan, WANG Hui, CHEN Zhi-qiang. Development of elite restoring lines by integrating blast resistance and low amylose content using MAS[J]. Journal of Integrative Agriculture, 2018, 17(01): 16-27.

参考文献

Ayres N M, McClung A M, Larkin P D, Bligh H F J, Jones C A, Park W D. 1997. Microsatellites and a single-nucleotide polymorphism differentiate apparent amylose classes in an extended pedigree of US rice germ plasm. Theoretical Applied Genetics, 94, 773–781.

Bao J S, Corke H, Sun M. 2006. Microsatellites, single nucleotide polymorphisms and a sequence tagged site in starch-synthesizing genes in relation to starch physicochemical properties in nonwaxy rice (Oryza sativa L.). Theoretical Applied Genetics, 113, 1185–1196.

Barman S R, Gowda M, Venu R C, Chattoo B B. 2004. Identification of a major blast resistance gene in the rice cultivar ‘Tetep’. Plant Breeding, 123, 300–302.

Basavaraj S H, Singh V K, Singh A, Anand D, Yadav S, Ellur R K, Singh D, Krishnan S G, Nagarajan M, Mohapatra T, Prabhu K V, Singh A K. 2010. Marker-assisted improvement of bacterial blight resistance in parental lines of Pusa RH10, a superfine grain aromatic rice hybrid. Molecular Breeding, 26, 293–305.

Bligh H F J, Till R I, Jones C A. 1995. A microsatellite sequence closely linked to the Waxy gene of Oryza sativa. Euphytica, 86, 83–85.

Bryan G T, Wu K S, Farrall L, Jia Y L, Hershey H P, McAdams S A, Faulk K N, Donaldson G K, Tarchini R, Valent B. 2000. A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta. The Plant Cell, 12, 2033–2045.

Cai H, Xie W, Lian X. 2013. Comparative analysis of differentially expressed genes in rice under nitrogen and phosphorus starvation stress conditions. Plant Molecular Biological Reports, 31, 160–173.

Chen D H, Vina M, Inukai T, Mackill D J, Ronald P C, Nelson R J. 1999. Molecular mapping of the blast resistance gene, Pi44(t) in a line derived from a durably resistant rice cultivar. Theoretical Applied Genetics, 98, 1046–1053.

Chen M H, Bergman C, Pinson S, Fjellstrom R. 2008. Waxy gene haplotypes: Associations with apparent amylose content and the effect by the environment in an international rice germplasm collection. Journal of Cereal Science, 47, 536–545.

Cheng F M, Zhong L J, Wang F, Zhang G P. 2005. Differences in cooking and eating properties between chalky and translucent parts in rice grains. Food Chemistry, 90, 39–46.

Couch B C, Kohn L M. 2002. A multilocus gene genealogy concordant with host preference indicates segregation of a new species Magnaporthe oryzae, from M. grisea. Mycologia, 94, 683–693.

Duan Y L, Guan H Z, Zhuo M, Chen Z W, Li W T, Pan R S, Mao D W, Zhuo Y C, Wu W R. 2012. Genetic analysis and mapping of an enclosed panicle mutant locus esp1 in rice (Oryza sativa L.). Journal of Integrative Agriculture, 11, 1933–1939.

Ellur R K, Khanna A, Yadav A, Pathania S, Rajashekara H, Singh V K, Gopala Krishnan S, Bhowmick P K, Nagarajan M, Vinod K K, Prakash G, Mondal K K, Singh N K, Vinod Prabhu K, Singh A K. 2016. Improvement of Basmati rice varieties for resistance to blast and bacterial blight diseases using marker assisted backcross breeding. Plant Science, 242, 330–341.

He Y, Han Y, Jiang L, Xu C, Lu J, Xu M. 2006. Functional analysis of starch synthesis genes in determining rice eating and cooking qualities. Molecular Breeding, 18, 277–290.

Hittalmani S, Parco A, Mew T V, Zeigler R S, Huang N. 2000. Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice. Theoretical Applied Genetics, 100, 1121–1128.

Hori K, Suzuki K, Iijima K, Ebana K. 2016. Variation in cooking and eating quality traits in Japanese rice germplasm accessions. Breeding Science, 66, 309–318.

Hospital F, Chevalet C, Mulsant P. 1992. Using markers in gene introgression breeding programs. Genetics, 132, 1199–1210.

IRRI (International Rice Research Institute). 2013. Standard Evaluation System (SES) for Rice. 5th ed. International Rice Research Institute, Manila. pp. 18–19.

Jairin J, Teangdeerith S, Leelagud P, Kothcharerk J, Sansen K, Yi M, Vanavichit A, Toojinda T. 2009. Development of rice introgression lines with brown planthopper resistance and KDML105 grain quality characteristics through marker-assisted selection. Field Crops Research, 110, 263–271.

Jantaboon J, Siangliw M, Immark S, Jamboonsri W, Vanavichit A, Toojind T. 2011. Ideotype breeding for submergence tolerance and cooking quality by marker-assisted selection in rice. Field Crops Research, 123, 206–213.

Jia Y, Wang Z, Singh P. 2002. Development of dominant rice blast Pi-ta resistance gene markers. Crop Science, 42, 2145–2149.

Jin L, Lu Y, Shao Y A, Zhang G, Xiao P, Shen S Q, Corke H, Bao J S. 2010. Molecular marker assisted selection for improvement of the eating, cooking and sensory quality of rice (Oryza sativa L.). Journal of Cereal Science, 51, 159–164.

Lanceras J C, Huang Z L, Naivikul O, Vanavichit A, Ruanjaichon V, Tragoonrung S. 2000. Mapping of genes for cooking and eating qualities in Thai jasmine rice (KDML 105). DNA Research, 7, 93–101.

Liu B, Zhang S H, Zhu X Y, Yang Q Y, Wu S Z, Mei M T, Mauleon R, Leach J, Mew T, Leung H. 2004. Candidate defense genes as predictors of quantitative blast resistance in rice. Molecular Plant Microbe Interactions, 17, 1146–1152.

Liu J L, Wang X J, Mitchell T, Hu Y J, Liu X L, Dai L Y, Wang G L. 2010. Recent progress and understanding of the molecular mechanisms of the rice - Magnaporthe oryzae interaction. Molecular Plant Pathology, 11, 419–427.

Mackill D J, Bonman J M. 1992. Inheritance of blast resistance in near-isogenic lines of rice. Phytopathology, 82, 746–749.

Murray M G, Thompson W F. 1980. Rapid isolation of high molecular-weight plant DNA. Nucleic Acids Research, 8, 4321–4325.

Nakamura S, Asakawa S, Ohmido N, Fukui K, Shimizu N, Kawasaki S. 1997. Construction of an 800-kb contig in the near-centromeric region of the rice blast resistance gene Pi-ta2

using a highly representative rice BAC library. Molecular Genetics Genomics, 254, 611–620.

Ni D, Song F, Ni J, Zhang A, Wang C, Zhao K, Yang Y, Wei P, Yang J, Li L. 2015. Marker-assisted selection of two-line hybrid rice for disease resistance to rice blast and bacterial blight. Field Crops Research, 184, 1–8.

Peng S B, Tang Q Y, Zou Y B. 2009. Current status and challenges of rice production in China. Plant Production Science, 12, 3–8.

Ramkumar G, Prahalada G D, Hechanova S L, Vinarao R, Jena K K. 2015. Development and validation of SNP-based functional codominant markers for two major disease resistance genes in rice (O. sativa L.). Molecular Breeding, 35, 129.

RoyChowdhury M, Jia Y L, Jackson A, Jia M H, Fjellstrom R, Cartwright R D. 2012. Analysis of rice blast resistance gene Pi-z in rice germplasm using pathogenicity assays and DNA markers. Euphytica, 184, 35–46.

Sallaud C, Lorieux M, Roumen E, Tharreau D, Berruyer R, Svestasrani P, Garsmeur O, Ghesquiere A, Notteghem J L. 2003. Identification of five new blast resistance genes in the highly blast-resistant rice variety IR64 using a QTL mapping strategy. Theorrtical Applied Genetics, 106, 794–803.

Skamnioti P, Gurr S J. 2009. Against the grain: Safeguarding rice from rice blast disease. Trends in Biotechnology, 27, 141–150.

Smith A M, Denyer K, Martin C. 1997. The synthesis of the starch granule. Annual Review Plant Physiology Plant Molecular Biology, 48, 67–87.

Tacconi G, Baldassarre V, Lanzanova C, Faivre-Rampant O, Cavigiolo S, Urso S, Lupotto E, Vale G. 2010. Polymorphism analysis of genomic regions associated with broad-spectrum effective blast resistance genes for marker development in rice. Molecular Breeding, 26, 595–617.

Tan Y F, Li J X, Yu S B, Xing Y Z, Xu C G, Zang Q. 1999. The three important traits for cooking and eating quality of rice grains are controlled by a single locus in an elite rice hybrid, Shanyou 63. Theoretical Applied Genetics, 99, 642–648.

Temnykh S, Park W D, Ayres N, Cartinhour S, Hauck N, Lipovich L, Cho Y G, Ishii T, McCouch S R. 2000. Mapping and genome organization of microsatellite sequences in rice (Oryzan sativa L.). Theoretical Applied Genetics, 100, 697–712.

Tsukaguchi T, Nitta S, Matsuno Y. 2016. Cultivar differences in the grain protein accumulation ability in rice (Oryza sativa L.). Field Crops Research, 192, 110–117.

Wang L Q, Liu W J, Xu Y, He Y Q, Luo L J, Xing Y Z, Xu C G, Zhang Q. 2007. Genetic basis of 17 traits and viscosity parameters characterizing the eating and cooking quality of rice grain. Theoretical Applied Genetics, 115, 463–476.

Wang Z, Jia Y, Rutger J N, Xia J. 2007. Rapid survey for presence of a blast resistance gene Pi-ta in rice cultivars using the dominant DNA markers derived from portions of the Pi-ta gene. Plant Breeding, 126, 36–42.

Wang Z Y, Zheng F Q, Shen G Z, Gao J P, Snustad D P, Li M G, Zhang J L, Hong M M. 1995. The amylose content in rice endosperm is related to the post-transcriptional regulation of the waxy gene. The Plant Journal, 7, 613–622.

Xiao W M, Luo L X, Wang H, Guo T, Liu Y Z, Zhou J Y, Zhu X Y, Yang Q Y, Chen Z Q. 2016. Pyramiding of Pi46 and Pita to improve blast resistance and to evaluate the resistance effect of the two R genes. Journal of Integrative Agriculture, 15, 2290–2298.

Xiao W M, Yang Q Y, Sun D Y, Wang H, Guo T, Liu Y Z, Zhu X Y, Chen Z Q. 2015. Identification of three major R genes responsible for broadspectrum blast resistance in an indica rice accession. Molecular Breeding, 35, 49.

Xiao W M, Yang Q Y, Wang H, Duan J, Guo T, Liu Y Z, Zhu X Y, Chen Z Q. 2012. Identification and fine mapping of a major R gene to Magnaporthe oryzae in a broad-spectrum resistant germplasm in rice. Molecular Breeding, 30, 1715–1726.

Xiao W M, Yang Q Y, Wang H, Guo T, Liu Y Z, Zhu X Y, Chen Z Q. 2011. Identification and fine mapping of a resistance gene to Magnaporthe oryzae in a space-induced rice mutant. Molecular Breeding, 28, 303–312.

Xu X, Hayashi N, Wang C T, Fukuoka S, Kawasaki S, Takatsuji H, Jiang C J. 2014. Rice blast resistance gene Pikahei-1(t), a member of a resistance gene cluster on chromosome 4, encodes a nucleotide-binding site and leucine-rich repeat protein. Molecular Breeding, 34, 691–700.

Yi M, Nwea K T, Vanavichit A, Chai-arree W, Toojinda T. 2009. Marker assisted backcross breeding to improve cooking quality traits in Myanmar rice cultivar Manawthukha. Field Crops Research, 113, 178–186.

Zhou L J, Liang S S, Ponce K, Marundon S, Ye G Y, Zhao X Q. 2015. Factors affecting head rice yield and chalkiness in indica rice. Field Crops Research, 172, 1–10.

Zhou P H, Tan Y F, He Y Q, Xu C G, Zhang Q. 2003. Simultaneous improvement for four quality traits of Zhenshan 97, an elite parent of hybrid rice, by molecular marker-assisted selection. Theoretical Applied Genetics, 106, 326–331.