Marker-assisted pyramiding of soybean resistance genes RSC4, RSC8, and RSC14Q to soybean mosaic virus

doi:10.1016/S2095-3119(17)61682-4

Journal of Integrative Agriculture ›› 2017, Vol. 16 ›› Issue (11): 2413-2420.DOI: 10.1016/S2095-3119(17)61682-4

收稿日期:2016-11-28 出版日期:2017-11-20 发布日期:2017-11-03

Marker-assisted pyramiding of soybean resistance genes R_SC4, R_SC8, and R_SC14Qto soybean mosaic virus

WANG Da-gang^{1, 2}, ZHAO Lin¹, LI Kai¹, MA Ying¹, WANG Li-qun¹, YANG Yong-qing¹, YANG Yun-hua¹, ZHI Hai-jian¹

¹College of Agriculture, Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, P.R.China
² Crop Institute, Anhui Academy of Agricultural Sciences/Anhui Key Laboratory of Crops Quality Improving, Hefei 230031, P.R.China

Received:2016-11-28 Online:2017-11-20 Published:2017-11-03
Contact: Correspondence ZHI Hai-jian, Tel: +86-25-84396463, E-mail: zhj@njau.edu.cn
About author:WANG Da-gang, E-mail: smvwang@163.com
Supported by:
This work was supported by the National Natural Science Foundation of China (31571687, 31571690, and 31371646), the Natural Science Foundation of Anhui Province, China (1708085MC69), the Jiangsu Collaborative Innovation Center for Modern Crop Production, China (JCIC-MCP), and the Fund of Transgenic Breeding for Soybean Resistance to Soybean Mosaic Virus, China (2016ZX08004-004).

摘要/Abstract

Abstract: Soybean mosaic virus (SMV) is one of the major viral pathogens affecting soybean crops worldwide. Three SMV resistance genes, R_SC4, R_SC8, and R_SC14Q, have been identified and mapped on soybean chromosomes 14, 2, and 13 from Dabaima, Kefeng 1, and Qihuang 1 cultivars, respectively. Soybean cultivar Nannong 1138-2 is widely grown in the Yangtze River Valley of China. In this study, crosses were made between Qihuang 1×Kefeng 1 and Dabaima×Nannong 1138-2. Ten simple sequence repeat (SSR) markers linked to three resistance loci (R_SC4, R_SC8, and R_SC14Q) were used to assist pyramided breeding. Pyramided families containing three resistance loci (R_SC4, R_SC8, and R_SC14Q) were evaluated by inoculating them with 21 SMV strains from China. Results indicated that the 10 markers can be used effectively to assist the selection of resistant individuals containing R_SC4, R_SC8, and R_SC14Q. A total of 53 F6 plants were confirmed to contain three homozygous alleles conferring resistance to SMV. Five F₇ homozygous pyramided families exhibited resistance to 21 strains of SMV and showed desirable agronomic traits using dual selection. The strategy of pyramiding resistance gene derived from different varieties has practical breeding value in providing broad-spectrum resistance against the existing strains of SMV in China.

Key words: soybean , soybean mosaic virus , resistance genes , pyramiding , marker-assisted breeding

. [J]. Journal of Integrative Agriculture, 2017, 16(11): 2413-2420.

WANG Da-gang, ZHAO Lin, LI Kai, MA Ying, WANG Li-qun, YANG Yong-qing, YANG Yun-hua, ZHI Hai-jian. Marker-assisted pyramiding of soybean resistance genes R_SC4, R_SC8, and R_SC14Qto soybean mosaic virus[J]. Journal of Integrative Agriculture, 2017, 16(11): 2413-2420.

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Marker-assisted pyramiding of soybean resistance genes R_SC4, R_SC8, and R_SC14Qto soybean mosaic virus

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