Inheritance and molecular characterization of resistance to AHAS-inhibiting herbicides in rapeseed

doi:10.1016/S2095-3119(17)61659-9

Journal of Integrative Agriculture ›› 2017, Vol. 16 ›› Issue (11): 2421-2433.DOI: 10.1016/S2095-3119(17)61659-9

收稿日期:2016-10-22 出版日期:2017-11-20 发布日期:2017-11-03

Inheritance and molecular characterization of resistance to AHAS-inhibiting herbicides in rapeseed

HU Mao-long, PU Hui-ming, GAO Jian-qin, LONG Wei-hua, CHEN Feng, ZHOU Xiao-ying, ZHANG Wei, PENG Qi, CHEN Song, ZHANG Jie-fu

Nanjing Sub-Center of the National Center, Oil Crops Improvement/Key Laboratory of Cotton and Rapeseed of the Ministry of Agriculture/Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, P.R.China

Received:2016-10-22 Online:2017-11-20 Published:2017-11-03
Contact: Correspondence PU Hui-ming, Tel: +86-25-84390370, Fax: +86-25-84390366, E-mail: puhuiming@jaas.ac.cn
About author:HU Mao-long, E-mail: humolon@163.com
Supported by:
This work was supported by the National Natural Science Foundation of China (31671731), the National Key Research and Development Program of China (2016YFD0101300), the China Agricultural Research System (CARS-13), the Natural Science Foundation of Jiangsu Province, China (BK20151369), and the Science Foundation of Jiangsu Academy of Agricultural Sciences, China (6111618). We also thank the Jiangsu Collaborative Innovation Centre for Modern Crop Production, China.

摘要/Abstract

Abstract: Rapeseed is a very important oil crop in China; however, its production is challenging due to the absence of effective weed management strategies. This is predominantly because of a shortage of herbicide resistance genes. Acetohydroxyacid synthase (AHAS) herbicides inhibit AHAS, a key enzyme involved in branched-chain amino acid synthesis that is required for plant growth. A rapeseed line designated M342 with AHAS herbicide resistance was developed through seed mutagenesis and was studied to assess the level and mode of inheritance of the resistance and to identify the molecular basis of resistance. M342 possessed a high level of cross-resistance to sulfonylureas (SUs) and imidazolinones (IMIs). This resistance was due to AHAS insensitivity to these herbicides and was inherited as a dominant trait conferred by a single nuclear-encoded gene. Molecular analysis revealed the presence of a Trp574Leu mutation in M342, and an allele-specific cleaved amplified polymorphic sequence (AS-CAPS) marker was developed and cosegregated with herbicide resistance in the F₂, BC₁, and BC₂ populations. This mutation altered the transcript levels of BnAHAS1 and BnAHAS3 in M342 compared with those in the wild type, but it did not affect the agronomic or quality traits. The simple genetic inheritance of this mutation and the availability of the cleaved amplified polymorphic sequence (CAPS) marker and herbicide resistance gene should facilitate the development of herbicide-resistant rapeseed cultivars for effective weed control in China.

Key words: rapeseed , acetohydroxyacid synthase (AHAS) , sulfonylurea , imidazolinone , herbicide resistance , mutation

. [J]. Journal of Integrative Agriculture, 2017, 16(11): 2421-2433.

HU Mao-long, PU Hui-ming, GAO Jian-qin, LONG Wei-hua, CHEN Feng, ZHOU Xiao-ying, ZHANG Wei, PENG Qi, CHEN Song, ZHANG Jie-fu. Inheritance and molecular characterization of resistance to AHAS-inhibiting herbicides in rapeseed[J]. Journal of Integrative Agriculture, 2017, 16(11): 2421-2433.

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Inheritance and molecular characterization of resistance to AHAS-inhibiting herbicides in rapeseed

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