Journal of Integrative Agriculture ›› 2017, Vol. 16 ›› Issue (07): 1558-1565.DOI: 10.1016/S2095-3119(16)61520-4

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  • 收稿日期:2016-05-29 出版日期:2017-07-20 发布日期:2017-07-06

Association of CYP19A1 gene polymorphisms with reproductive traits in pigs

ZHOU Rong1, 2, YANG Ya-lan2, LIU Ying2, CHEN Qi-mei3, CHEN Jie1, LI Kui1, 2   

  1. 1 Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, P.R.China
    2 Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
    3 Shandong Lansi Seeds Industry Co., Ltd., Rizhao 276800, P.R.China
  • Received:2016-05-29 Online:2017-07-20 Published:2017-07-06
  • Contact: Correspondence CHEN Jie, Tel/Fax: +86-25-84396347, E-mail: jiechen@njau.edu.cn; LI Kui, Tel/Fax: +86-10-62813822, E-mail: likui@caas.cn
  • About author:ZHOU Rong, E-mail: zhourong03@caas.cn;
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (31501931), the 948 Program of China (2011-G11), and the Agricultural Science and Technology Innovation Program, China (ASTIP-IAS05).

Abstract:     Porcine reproductive traits are characterized by low heritability, making improvement by traditional selective breeding rather difficult. Molecular breeding offers powerful approaches to overcome previous limitations and is expected to generate economic benefits via progress in pig breeding. Cytochrome P450 family 19 subfamily A polypeptide 1 (CYP19A1) gene is a key enzyme of estradiol biosynthesis that plays an important role in the establishment of gestation and maintenance of pregnancy. In this study, the sequence and structure characteristics of the porcine CYP19A1 gene was analyzed and expression patterns of CYP19A1 in different tissues of adult female pigs were detected. Fourteen single-nucleotide polymorphisms (SNPs) in the exons and introns of porcine CYP19A1 were identified and genotyped using the Sequenom MassARRAY platform, after which the allele frequency of each SNP was analyzed. The association between CYP19A1 SNPs and litter size and piglet birth weight was assessed in a crossbred pig population (n=375). The expression pattern of CYP19A1 revealed that it was highly expressed in the ovary, spleen, and uterus and lowly expressed in the other tissues. Moreover, one SNP, rs341891833, was significantly associated with piglet birth weight during the multiparity period (P<0.01). We concluded that CYP19A1 could be used as a candidate molecular marker in breeding aimed at rapid improvement of the reproductive characteristics of pigs.  

Key words: CYP19A1 ,  pig ,  reproduction ,  birth weight ,  single nucleotide polymorphism