A method to evaluate the bioactive function of fruit extracts of Chinese wild Citrus with microtubular activity

doi:10.1016/S2095-3119(16)61463-6

Journal of Integrative Agriculture ›› 2017, Vol. 16 ›› Issue (04): 867-873.DOI: 10.1016/S2095-3119(16)61463-6

收稿日期:2016-05-19 出版日期:2017-04-04 发布日期:2017-04-07

A method to evaluate the bioactive function of fruit extracts of Chinese wild Citrus with microtubular activity

GUAN Xin^1,^4*, TAN Si^1,^2*, Günther Buchholz³, Peter Nick², ZHOU Zhi-qin^1,⁵

¹ College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, P.R.China
² Molecular Cell Biology, Botanical Institute, Karlsruhe Institute of Technology, Karlsruhe D-76128, Germany
³RLP AgroScience/AlPlanta-Institute for Plant Research, Neustadt an der Weinstrasse D-67435, Germany
⁴ Laboratoire Vigne Biotechnologies et Environnement EA-3991, Université de Haute-Alsace, Colmar 68000, France
⁵ Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400716, P.R.China

Received:2016-05-19 Online:2017-04-04 Published:2017-04-07
Contact: GUAN Xin, E-mail: xinguan.cnde@googlemail.com; ZHOU Zhi-qin, Tel: +86-23-68250229, Fax: +86-23-68251274, E-mail: zhouzhiqin@swu.edu.cn
Supported by:
This work was supported by the Fundamental Research Funds for the Central Universities, China (SWU113107 and XDJK2014A014), the Programs for Research on the Teaching Reform of Graduate Education and Basic Science and Cutting-edge Technology Research (yjg153078 and cstc2016jcyjA0489) in Chongqing, China. A fellowship of Chinese “111” project (B12006) to Mr. Tan Si in lab of Prof. Peter Nick, Molecular Cell Biology, Botanical Institute, Karlsruhe Institute of Technology, Germany.

摘要/Abstract

Abstract: China is one of the most important centers of origin for Citrus genetic resources. Due to the high content of secondary metabolites, mining wild Chinese Citrus for novel medical applications is promising. In this study, extracts of Chinese wild species from different taxonomical groups were screened for potential effects on microtubules (MTs) in vitro. MT density as a readout for nucleation, and frequency distribution over MT lengths as a readout for elongation and decay were determined by quantitative image analysis via a standardized coverslip assay using fluorescently labelled neurotubulin. Extract from peels of Citrus ichangensis Swing. strongly increased the density of MTs; whereas, extract from peels of Citurs limon (L.) Burm.f. exerted the opposite effect. Extract from pulp of Citrus limonia Osbeck promoted MT elongation, and in addition induced a small population of very long MTs. These data suggest that wild Chinese Citrus harbour compounds that act specifically on different aspects of MT nucleation, elongation, and decay.

Key words: fruit extract, natural product-based pharmaceuticals, Chinese wild Citrus, microtubules secondary plant , metabolites

. [J]. Journal of Integrative Agriculture, 2017, 16(04): 867-873.

GUAN Xin, TAN Si, Günther Buchholz, Peter Nick, ZHOU Zhi-qin. A method to evaluate the bioactive function of fruit extracts of Chinese wild Citrus with microtubular activity[J]. Journal of Integrative Agriculture, 2017, 16(04): 867-873.

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A method to evaluate the bioactive function of fruit extracts of Chinese wild Citrus with microtubular activity

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