The allelic distribution and variation analysis of the NAM-B1 gene in Chinese wheat cultivars

doi:10.1016/S2095-3119(16)61459-4

Journal of Integrative Agriculture ›› 2017, Vol. 16 ›› Issue (06): 1294-1303.DOI: 10.1016/S2095-3119(16)61459-4

出版日期:2017-06-20 发布日期:2017-06-08

The allelic distribution and variation analysis of the NAM-B1 gene in Chinese wheat cultivars

CHEN Xue-yan^{1, 2*}, SONG Guo-qi^2*, ZHANG Shu-juan², LI Yu-lian², GAO Jie², Islam Shahidul^{3, 4}, MA Wu-jun^{3, 4}, LI Gen-ying², JI Wan-quan¹

¹College of Agronomy, Northwest A&F University, Yangling 712100, P.R.China
²Crop Research Institute, Shandong Academy of Agricultural Sciences/Key Laboratory of Wheat Biology & Genetic Improvement on North Yellow & Huai River Valley, Ministry of Agriculture/National Engineering Laboratory for Wheat & Maize, Jinan 250100, P.R.China
³Australia-China Centre for Wheat Improvement, School of Veterinary and Life Sciences, Murdoch University, Perth WA 6150, Australia
⁴Australia Export Grains Innovation Centre, 3 Baron-Hay Court, Perth WA 6151, Australia

Online:2017-06-20 Published:2017-06-08
Contact: LI Gen-ying, Tel: +86-531-83178122, E-mail: lgy111@126.com; JI Wan-quan, Tel: +86-29-87081319, E-mail: jiwanquan2003@126.com
About author:CHEN Xue-yan, E-mail: CHXYTVS@163.com; SONG Guo-qi, E-mail: song_guoqi@126.com
Supported by:
This research was supported by the National Natural Science Founding of China (31401378), the Major Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences, China (2014CXZ10), the Science & Technology Development Plan of Shandong Province, China (2014GSF121001), the Youth Foundation of Shandong Academy of Agricultural Sciences, China (2014QNZ02), and the Program for Youth Talent of Shandong Academy of Agricultural Sciences, China (118005).

摘要/Abstract

Abstract: The NAM-B1 gene is a member of the NAC (NAM, ATAF, and CUC) transcription factor family and plays an important role in regulating wheat grain protein content (GPC). The ancestral NAM-B1 allele has been discovered in many tetraploid wild emmer (Triticum turgidum ssp. dicoccoides) accessions and few domesticated emmer accessions (T. turgidum ssp. dicoccum), however, it is rarely found in hexaploid bread wheat (Triticum aestivum L.). There are no systematic reports on the distribution of NAM-B1 alleles in Chinese wheat cultivars. In this study, the NAM-B1 alleles in 218 Chinese cultivars were investigated. The cultivars were collected from five major wheat regions (12 provinces), covering most of the winter wheat growing regions in China. The results showed that the NAM-B1 gene is present in 53 (24.3%) cultivars and absent in the remaining 165 (75.7%) cultivars. Further analysis revealed that in contrast to the wild-type allele, the NAM-B1 gene in Chinese wheat cultivars contained a 1-bp insertion in the coding region. This caused a frame-shift mutation and introduced a stop codon in the middle of the gene, rendering it non-functional. Polymorphisms were detected in DNA sequences of 21 cultivars among these 53 cultivars. However, cDNA sequence analysis suggested that these variations in the exon region were not able to restore NAM-B1 gene (1-bp insertion) function. Thus, exploring the distribution of NAM-B1 gene variations (1-bp insertion and deletion) can provide some information for improving the quality of winter wheat in China and other countries.

. [J]. Journal of Integrative Agriculture, 2017, 16(06): 1294-1303.

CHEN Xue-yan, SONG Guo-qi, ZHANG Shu-juan, LI Yu-lian, GAO Jie, Islam Shahidul, MA Wu-jun, LI Gen-ying, JI Wan-quan. The allelic distribution and variation analysis of the NAM-B1 gene in Chinese wheat cultivars[J]. Journal of Integrative Agriculture, 2017, 16(06): 1294-1303.

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The allelic distribution and variation analysis of the NAM-B1 gene in Chinese wheat cultivars

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