Chromosome painting of telomeric repeats reveals new evidence for genome evolution in peanut

doi:10.1016/S2095-3119(16)61423-5

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (11): 2488-2496.DOI: 10.1016/S2095-3119(16)61423-5

收稿日期:2015-12-01 出版日期:2016-11-04 发布日期:2016-11-04

Chromosome painting of telomeric repeats reveals new evidence for genome evolution in peanut

DU Pei^{1, 2*}, LI Li-na^{2, 3*}, ZHANG Zhong-xin², LIU Hua², QIN Li², HUANG Bing-yan², DONG Wen-zhao², TANG Feng-shou², QI Zeng-jun¹, ZHANG Xin-you²

¹ State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, P.R.China
² Industrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou 450002, P.R.China
³ College of Agriculture, Henan University of Science and Technology, Luoyang 471023, P.R.China

Received:2015-12-01 Online:2016-11-04 Published:2016-11-04
Contact: QI Zeng-jun, Tel: +86-25-84399029, Fax: +86-25-84395344, E-mail: zjqi@njau.edu.cn; ZHANG Xin-you, Tel: +86-371-65729560, E-mail: haasz@126.com
Supported by:
This research was supported by the China Agriculture Research System (CARS-14), the Henan Provincial Agriculture Research System, China (S2012-05) and the Major Technology Research and Development Program of Henan Province, China (141100110600).

摘要/Abstract

Abstract: Interspecific hybridization is an important approach to improve cultivated peanut varieties. Cytological markers such as tandem repeats will facilitate alien gene introgression in peanut. Telomeric repeats have also been frequently used in chromosome research. Most plant telomeric repeats are (TTTAGGG)_n that are mainly distributed at the chromosome ends, although interstitial telomeric repeats (ITRs) are also commonly identified. In this study, the telomeric repeat was chromosomally localized in 10 Arachis species through sequential GISH (genomic in situ hybridization) and FISH (fluorescence in situ hybridization) combined with 4’,6-diamidino-2-phenylindole (DAPI) staining. Six ITRs were identified such as in the centromeric region of chromosome Bⁱ5 in Arachis ipaënsis, pericentromeric regions of chromosomes A^s5 in A. stenosperma, B^ho7 in A. hoehnei and A^v5 in A. villosa, nucleolar organizer regions of chromosomes A^s3 in A. stenosperma and A^di3 in A. diogoi, subtelomeric regions of chromosomes B^ho9 in A. hoehnei and A^du7 in A. duranensis, and telomeric region of chromosome E^s7 in A. stenophylla. The distributions of the telomeric repeat, 5S rDNA, 45S rDNA and DAPI staining pattern provided not only ways of distinguishing different chromosomes, but also karyotypes with a higher resolution that could be used in evolutionary genome research. The distribution of telomeric repeats, 5S rDNA and 45S rDNA sites in this study, along with inversions detected on the long arms of chromosomes K^b10 and B^ho10, indicated frequent chromosomal rearrangements during evolution of Arachis species.

Key words: Arachis species , inversion , interstitial telomeric repeats , karyotype

DU Pei, LI Li-na, ZHANG Zhong-xin, LIU Hua, QIN Li, HUANG Bing-yan, DONG Wen-zhao, TANG Feng-shou, QI Zeng-jun, ZHANG Xin-you. [J]. Journal of Integrative Agriculture, 2016, 15(11): 2488-2496.

DU Pei, LI Li-na, ZHANG Zhong-xin, LIU Hua, QIN Li, HUANG Bing-yan, DONG Wen-zhao, TANG Feng-shou, QI Zeng-jun, ZHANG Xin-you. Chromosome painting of telomeric repeats reveals new evidence for genome evolution in peanut[J]. Journal of Integrative Agriculture, 2016, 15(11): 2488-2496.

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Chromosome painting of telomeric repeats reveals new evidence for genome evolution in peanut

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