Impacts of the unsaturation degree of long-chain fatty acids on the volatile fatty acid profiles of rumen microbial fermentation in goats in vitro

doi:10.1016/S2095-3119(16)61418-1

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (12): 2827-2833.DOI: 10.1016/S2095-3119(16)61418-1

收稿日期:2015-12-02 出版日期:2016-12-01 发布日期:2016-12-02

Impacts of the unsaturation degree of long-chain fatty acids on the volatile fatty acid profiles of rumen microbial fermentation in goats in vitro

GAO Jian^1*, WANG Meng-zhi^1*, JING Yu-jia¹, SUN Xue-zhao², WU Tian-yi¹, SHI Liang-feng¹

¹ College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China
² Grasslands Research Centre, AgResearch Ltd., Private Bag 11008, Palmerston North, New Zealand

Received:2015-12-02 Online:2016-12-01 Published:2016-12-02
Contact: WANG Meng-zhi, Tel/Fax: +86-514-87997196, E-mail: mengzhiwangyz @126.com
About author:GAO Jian, E-mail: gaojianyzu@126.com;
Supported by:
The study was financially supported by the Graduate Student Innovation Project of Jiangsu Province, China (KYLX15_1377), the Natural Science Foundation of Jiangsu Province, China (BK20151312), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), China.

摘要/Abstract

Abstract: This study investigated the impacts of the degree of unsaturation (unsaturity) of long-chain fatty acids on volatile fatty acid (VFA) profiles of rumen fermentation in vitro. Six types of long-chain fatty acids, including stearic acid (C18:0, control group), oleic acid (C18:1, n-9), linoleic acid (C18:2, n-6), α-linolenic acid (C18:3, n-3), arachidonic acid (C20:4, n-6) and eicosapentaenoic acid (C20:5, n-3), were tested. Rumen fluid from three goats fitted with ruminal fistulae was used as inoculum and the inclusion rate of long-chain fatty acid was at 3% (w/w) of substrate. Samples were taken for VFA analysis at 0, 3, 6, 9, 12, 18 and 24 h of incubation, respectively. The analysis showed that there were significant differences in the total VFA among treatments, sampling time points, and treatment×time point interactions (P<0.01). α-Linolenic acid had the highest total VFA (P<0.01) among different long-chain fatty acids tested. The molar proportion of acetate in total VFA significantly differed among treatments (P<0.01) and sampling time points (P<0.01), but not treatment×time point interactions (P>0.05). In contrast, the molar proportion of propionate did not differ among treatments during the whole incubation (P>0.05). However, for butyrate molar proportions, significant differences were found not only among sampling time points but also among treatments and treatment×time point interactions (P<0.01), with eicosapentaenoic acid having the highest value (P<0.01). Additionally, no statistically significant differences were found in the acetate to propionate ratios among treatments groups (P>0.05), even the treatments stearic acid and α-linolenic acid were numerically higher than the others. The inclusion of 3% long-chain unsaturated fatty acids differing in the degree of unsaturation brought out a significant quadratic regression relation between the total VFA concentration and the double bond number of fatty acid. In conclusion, the α-linolenic acid with 3 double bonds appeared better for improving rumen microbial fermentation and the total VFA concentration.

Key words: volatile fatty acid , unsaturation degree , long-chain fatty acid , in vitro fermentation

GAO Jian, WANG Meng-zhi, JING Yu-jia, SUN Xue-zhao, WU Tian-yi, SHI Liang-feng. [J]. Journal of Integrative Agriculture, 2016, 15(12): 2827-2833.

GAO Jian, WANG Meng-zhi, JING Yu-jia, SUN Xue-zhao, WU Tian-yi, SHI Liang-feng. Impacts of the unsaturation degree of long-chain fatty acids on the volatile fatty acid profiles of rumen microbial fermentation in goats in vitro[J]. Journal of Integrative Agriculture, 2016, 15(12): 2827-2833.

参考文献

Bannink A, France J, Lopez S, Gerrits W J J, Kebreab E, Tamminqa S, Dijkstra J. 2008. Modelling the implications of feeding strategy on rumen fermentation and functioning of the rumen wall. Animal Feed Science and Technology, 143, 3–26.

Bensadoun A, Paladines O L, Reid J T. 1962. Effect of level of intake and physical form of the diet on plasma glucose concentration and volatile fatty acid absorption in ruminants. Journal of Dairy Science, 45, 1203–1210.

Chalupa W, Rickabaugh B, Kronfeld D, Sklan D. 1984. Rumen fermentation in vitro as influenced by long-chain fatty acids. Journal of Dairy Science, 67, 1439–1444.

Chalupa W, Vecchiarelli B, Elser A E, Kronfeld D S. 1986. Rumen fermentation in vivo as influenced by long-chain fatty acids. Journal of Dairy Science, 69, 1293–1301.

Dijkstra J, Gerrits W J J, Bannink A, France J. 2000. Modelling lipid metabolism in the rumen. In: McNamara J P, France J, Beever D E, eds., Modelling Nutrient Utilization in Farm Animals. CABI Publishing. pp. 25–36.

Faciola A P, Broderick G A. 2014. Effects of feeding lauric acid or coconut oil ruminal protozoa numbers, fermentation pattern, digestion, omasal nutrient flow, and milk production in dairy cows. Journal of Dairy Science, 97, 5088–5100.

Ferguson J D, Sklan D, Chalupa W V, Kronfeld D S. 1990. Effects of hard fats on in vitro and in vivo rumen fermentation, milk production, and reproduction in dairy cows. Journal of Dairy Science, 73, 2864–2879.

Feng Y L. 2004. Ruminant Nutrition. Science Press, Beijing. (in Chinese)

Firkins J L, Yu Z, Morrison M. 2007. Ruminal nitrogen metabolism: Perspectives for integration of microbiology and nutrition for dairy. Journal of Dairy Science, 90, E1–E16.

Fujihara T, Maeda S, Matsui T, Naruse H. 1996. The effect of treated (spray-dried) beef-tallow supplementation on feed digestion, ruminal fermentation and fat nutrition in sheep. Animal Science and Technology (Japan), 67, 14–23.

Gao J, Jing Y J, Wang M Z, Shi L F, Liu S M. 2016. The effects of the unsaturated degree of long-chain fatty acids on the rumen microbial protein content and the activities of transaminases and dehydrogenase in vitro. Journal of Integrative Agriculture, 15, 424–431.

Girard V, Hawke J C. 1978. The role of Holotrichs in the metabolism of dietary linoleic acid in the rumen. Biochimica et Biophysica Acta, 528, 17–27.

Ivan M, Petit H V, Chiquette J, Wright A D G. 2013. Rumen fermentation and microbial population in lactating dairy cows receiving diets containing oilseeds rich in C-18 fatty acids. British Journal of Nutrition, 109, 1211–1218.

Jenkins T C. 1993. Lipid metabolism in the rumen. Journal of Dairy Science, 76, 3851–3863.

Jal? D, ?erešňáková Z. 2002. Effect of plant oil and malate on rumen fermentation in vitro. Czech Journal of Animal Science, 47, 106–111.

Jal? D, ?ertík M, Kundrikova K, Kubelková P. 2009. Effect of microbial oil and fish oil on rumen fermentation and metabolism of fatty acids in artificial rumen. Czech Journal of Animal Science, 54, 229–237.

Li D, Wang J, Li F, Bu D. 2012. Document effects of malic acid and unsaturated fatty acids on methanogenesis and fermentation by ruminal microbiota in vitro. Journal of Animal and Veterinary Advances, 11, 2917–2922.

Li D, Wang J Q, Liu L, Liu K L, Yu P, Li F D. 2009. Effect of supplementing malic acid and unsaturated fat acid on rumen fermentation and functional microbe in vitro. Journal of Agricultural Biotechnology, 17, 1013–1019. (in Chinese)

Liu S J, Bu D P, Wang J Q, Sun P, Wei H Y, Zhou L Y, Yu Z T. 2011. Effect of ruminal pulse dose of polyunsaturated fatty acids on ruminal microbial populations and duodenal flow and milk profiles of fatty acids. Journal of Dairy Science, 94, 2977–2985.

Machmüller A, Ossowski D A, Wanner M, Kreuzer M. 1998. Potential of various fatty feeds to reduce methane release from rumen fermentation in vitro (Rusitec). Animal Feed Science and Technology, 71, 117–130.

McGinn S M, Beauchemin K A, Coates T. 2004. Methane emissions from beef cattle: Effects of monensin, sunflower oil, enzymes, yeast, and fumaric acid. Journal of Animal Science, 82, 3346–3356.

Menke K H, Steingass H. 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research Development, 28, 7–55.

Newbold C J, Chamberlain D G. 1988. Lipids as rumen defaunating agents. The Proceedings of the Nutrition Society, 47, 154.

Potu R B, Abu-Ghazaleh A A, Hastings D, Jones K, Ibrahim S A. 2011. The effect of lipid supplements on ruminal bacteria in continuous culture fermenters varies with the fatty acid composition. The Journal of Microbiology, 49, 216–223.

Tackett V L, Bertrand J A, Jenkins T C, Pardue F E, Grimes L W. 1996. Interaction of dietary fat and acid detergent fiber diets of lactating dairy cows. Journal of Dairy Science, 79, 270–275.

Van H M. 1996. Challenging the retinal for altering VFA ratios in growing ruminates. Feed Mix, 4, 514–525.

Wang M Z, Cheng X, Xie W W, Zhang B L, Liu X, Wang H R. 2010. Effects of different oils on bacteria recycling due to predation by rumen protozoa in vitro. Scientia Agricultura Sinica, 43, 3831–3837. (in Chinese)

Williams A G. 1989. Metabolic activities of rumen protozoa. In: Nolan J V, Leng R A, Demeyer D I, eds., The Role of Protozoa and Fungi in Ruminant Digestion. Penambul Books, Armidale, NSW. pp. 1197–1216.

Williams A G, Coleman G S. 1992. The Rumen Protozoa. Springer–Verlag, Netherlands.

Xiong B H, Lu D X, Gao J. 1999. Study on the absorption and relevant model parameters of VFA in the rumen of sheep. Acta Zoonutrimenta Sinica, 11, 248–255. (in Chinese)

Zhang C M, Guo Y Q, Yuan Z P, Wu Y M, Wang J K, Liu J X, Zhu W Y. 2008. Effect of octadeca carbon fatty acids on microbial fermentation, methanogenesis and microbial flora in vitro. Animal Feed Science and Technology, 146, 259–269.

Zhao Y H, Yang R H, Wang J Q. 2005. Methane production mechanism and regulation of rumen microbes. Journal of Microbiology, 25, 68–73.

Impacts of the unsaturation degree of long-chain fatty acids on the volatile fatty acid profiles of rumen microbial fermentation in goats in vitro

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics