Initial detection of the quorum sensing autoinducer activity in the rumen of goats in vivo and in vitro

doi:10.1016/S2095-3119(16)61417-X

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (10): 2343-2352.DOI: 10.1016/S2095-3119(16)61417-X

收稿日期:2015-10-29 出版日期:2016-10-01 发布日期:2016-10-01

Initial detection of the quorum sensing autoinducer activity in the rumen of goats in vivo and in vitro

RAN Tao^{1, 2}, ZHOU Chuan-she^{1, 3}, XU Li-wei¹, GENG Mei-mei¹, TAN Zhi-liang^{1, 3}, TANG Shao-xun^{1, 3}, WANG Min^{1, 3}, HAN Xue-feng^{1, 4}, KANG Jin-he¹

¹ Key Laboratory for Agro-Ecological Processes in Subtropical Region/Hunan Research Center of Livestock & Poultry Sciences/South-Central Experimental Station of Animal Nutrition and Feed Science, Ministry of Agriculture/Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, P.R.China
² University of the Chinese Academy of Sciences, Beijing 100049, P.R.China
³ Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Changsha 410128, P.R.China
⁴ Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Changsha 410128, P.R.China

Received:2015-10-29 Online:2016-10-01 Published:2016-10-01
Contact: TAN Zhi-liang, Tel: +86-731-84619702, Fax: +86-731-84612685, E-mail: zltan@isa.ac.cn
About author:RAN Tao, Mobile: +86-13755047025, E-mail: xinweiyuejun@126.com
Supported by:
The author gratefully acknowledges the financially support of the Chinese Academy of Sciences (KZCX2-YW-455), the CAS/SAFEA International Partnership Program for Creative Research Teams, China (KZCX2-YW-T07) and K C Wong Education, Hong Kong.

摘要/Abstract

Abstract: Quorum sensing (QS) is a type of microbe-microbe communication system that is widespread among the microbial world, particularly among microorganisms that are symbiotic with plants and animals. Thereby, the cell-cell signalling is likely to occur in an anaerobic rumen environment, which is a complex microbial ecosystem. In this study, using six ruminally fistulated Liuyang black goats as experimental animals, we aimed to detect the activity of quorum sensing autoinducers (AI) both in vivo and in vitro and to clone the luxS gene that encoded autoinducer-2 (AI-2) synthase of microbial samples that were collected from the rumen of goats. Neutral detergent fiber (NDF) and soluble starch were the two types of substrates that were used for in vitro fermentation. The fermented fluid samples were collected at 0, 2, 4, 6, 8, 12, 24, 36, and 48 h of incubation. The acyl-homoserine lactones (AHLs) activity was determined using gas chromatography-mass spectrometer (GC-MS) analysis. However, none of the rumen fluid extracts that were collected from the goat rumen showed the same or similar fragmentation pattern to AHLs standards. Meanwhile, the AI-2 activity, assayed using a Vibrio harveyi BB170 bioassay, was negative in all samples that were collected from the goat rumen and from in vitro fermentation fluids. Our results indicated that the activities of AHLs and AI-2 were not detected in the ruminal contents from six goats and in ruminal fluids obtained from in vitro fermentation at different sampling time-points. However, the homologues of luxS in Prevotella ruminicola were cloned from in vivo and in vitro ruminal fluids. We concluded that AHLs and AI-2 could not be detected in in vivo and in vitro ruminal fluids of goats using the current detection techniques under current dietary conditions. However, the microbes that inhabited the goat rumen had the potential ability to secrete AI-2 signaling molecules and to communicate with each other via AI-2-mediated QS because of the presence of luxS.

Key words: quorum sensing , AHLs , AI-2 , luxS , rumen bacteria, goat

RAN Tao, ZHOU Chuan-she, XU Li-wei, GENG Mei-mei, TAN Zhi-liang, TANG Shao-xun, WANG Min, HAN Xue-feng, KANG Jin-he. [J]. Journal of Integrative Agriculture, 2016, 15(10): 2343-2352.

RAN Tao, ZHOU Chuan-she, XU Li-wei, GENG Mei-mei, TAN Zhi-liang, TANG Shao-xun, WANG Min, HAN Xue-feng, KANG Jin-he. Initial detection of the quorum sensing autoinducer activity in the rumen of goats in vivo and in vitro[J]. Journal of Integrative Agriculture, 2016, 15(10): 2343-2352.

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Initial detection of the quorum sensing autoinducer activity in the rumen of goats in vivo and in vitro

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