Sequence and phylogenetic analysis of hemagglutinin genes of H9N2 influenza viruses isolated from chicken in China from 2013 to 2015

doi:10.1016/S2095-3119(16)61406-5

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (11): 2604-2612.DOI: 10.1016/S2095-3119(16)61406-5

收稿日期:2015-12-02 出版日期:2016-11-04 发布日期:2016-11-04

Sequence and phylogenetic analysis of hemagglutinin genes of H9N2 influenza viruses isolated from chicken in China from 2013 to 2015

SU Xiao-na^{1, 2, 3}, XIE Qing-mei^{1, 2, 3}, LIAO Chang-tao⁴, YAN Zhuan-qiang⁴, CHEN Wei-guo¹, BI Ying-zuo¹, CHEN Feng^{1, 2, 3}

¹ College of Animal Science, South China Agricultural University/Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou 510642, P.R.China
² Guangdong Provincial Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, P.R.China
³ Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, P.R.China
⁴ Guangdong Wen’s Foodstuffs Group Co. Ltd., Yunfu 527439, P.R.China

Received:2015-12-02 Online:2016-11-04 Published:2016-11-04
Contact: CHEN Feng, Tel: +86-20-85280283, Fax: +86-20-85280740, E-mail: fengch@scau.edu.cn
About author:SU XIAO-na, E-mail: 15989176454@163.com;
Supported by:
This work was supported by the National Modern Agricultural Industry Technology System Project of China (CARS-41) and the Science and Technology Plan Project of Guangdong Province, China (2012B020306002 and 2012B091100078).

摘要/Abstract

Abstract: H9N2 avian influenza virus (AIV) infection is a major problem in poultry industry worldwide. In this study, molecular characterizations and phylogenetic relationships of hemagglutinin (HA) gene sequences of H9N2 AIV of 5 Chinese isolates in 2014 recently available in GenBank, 3 widely used vaccine strains, and 52 novel isolates in China from 2013 to 2015 were analyzed. The homology analysis showed that the nucleotide sequences of HA gene of these recent Chinese H9N2 AIV isolates shared homologies from 94.1 to 99.9%. Phylogenetic analysis showed that all isolates belonged to AIV lineage h9.4.2.5. Fifty-six out of the 57 recent Chinese H9N2 AIV isolates had the motifs PSRSSR↓GLF at the cleavage sites within the HA protein, while one isolate PWH01 harbored LSRSSR↓GLF. Remarkably, all of the recent Chinese H9N2 AIV strains had the Q216L substitution in the receptor binding site, which indicated that they had potential to infect humans. Most of recent Chinese H9N2 AIV isolates lost the potential N-linked glycosylation site at residues 200–202 compared with vaccine strains. This present study demonstrated that AIV lineage h9.4.2.5 was more predominant in China than other lineages as it harbored all the H9N2 AIV isolated between 2013 and 2015. Also we showed the importance of continuous surveillance of emerging H9N2 AIV in China and update of vaccine formulation accordingly in order to prevent and control H9N2 AIV.

Key words: avian influenza virus , H9N2 subtype , phylogenetic analysis , hemagglutinin gene

SU Xiao-na, XIE Qing-mei, LIAO Chang-tao, YAN Zhuan-qiang, CHEN Wei-guo, BI Ying-zuo, CHEN Feng. [J]. Journal of Integrative Agriculture, 2016, 15(11): 2604-2612.

SU Xiao-na, XIE Qing-mei, LIAO Chang-tao, YAN Zhuan-qiang, CHEN Wei-guo, BI Ying-zuo, CHEN Feng. Sequence and phylogenetic analysis of hemagglutinin genes of H9N2 influenza viruses isolated from chicken in China from 2013 to 2015[J]. Journal of Integrative Agriculture, 2016, 15(11): 2604-2612.

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Sequence and phylogenetic analysis of hemagglutinin genes of H9N2 influenza viruses isolated from chicken in China from 2013 to 2015

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