SlMYB1 and SlMYB2, two new MYB genes from tomato, transcriptionally regulate cellulose biosynthesis in tobacco

doi:10.1016/S2095-3119(16)61389-8

摘要/Abstract

Abstract: Cellulose, a major constituent of plant biomass, is synthesized by a cellulose synthase complex. It has been demonstrated that MYB genes transcriptionally regulate cellulose synthase in Arabidopsis. However, little is known about this process in tomato. Here, two MYB (SlMYB1/2) and three cellulose synthase (CESA) (SlCESA4/5/6) genes were isolated. SlMYB1/2 and SlCESA4/5/6 accumulation was found to correspond to cellulose accumulation in different tissues of tomato. Dual luciferase assays indicated that these two MYBs were transcriptional activators that interact with promoters of SlCESA4/5/6. Moreover, SlMYB2 could also activate promoters of SlMYB1/2, suggesting the possible underlying auto-activation mechanisms for MYB transcription factors. Transient over-expression of SlMYB1/2 in Nicotiana tabacum up-regulated tobacco endogenous NtCESA genes and increased cellulose accumulation. The function of SlMYB1/2 was further investigated using stable transformation and the results indicated that N. tabacum lines heterologous expressing SlMYB1/2 displayed a pleiotropic phenotype, long and narrow leaves, with NtCESA induced and significant increase of cellulose. In conclusion, our data suggest that tomato SlMYB1/2 have transcriptional regulatory roles in cellulose biosynthesis and SlMYB2 was more effective than SlMYB1, which may due to the transcriptional activation by SlMYB2 on SlMYB1 and itself.

Key words: MYB, cellulose, cellulose synthase, transcriptional regulation, tomato, tobacco

SHI Yan-na, LIU Xiao-fen, LI Xue, DONG Wen-cheng, Donald Grierson, YIN Xue-ren, CHEN Kun-song . [J]. Journal of Integrative Agriculture, 2017, 16(01): 65-75.

SHI Yan-na, LIU Xiao-fen, LI Xue, DONG Wen-cheng, Donald Grierson, YIN Xue-ren, CHEN Kun-song . SlMYB1 and SlMYB2, two new MYB genes from tomato, transcriptionally regulate cellulose biosynthesis in tobacco[J]. Journal of Integrative Agriculture, 2017, 16(01): 65-75.

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