Genetic characterization of antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis cases in Northwest China

doi:10.1016/S2095-3119(16)61368-0

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (12): 2842-2847.DOI: 10.1016/S2095-3119(16)61368-0

收稿日期:2015-12-30 出版日期:2016-12-01 发布日期:2016-12-02

Genetic characterization of antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis cases in Northwest China

YANG Feng^1*, WANG Qi^2*, WANG Xu-rong¹, WANG Ling¹, LI Xin-pu¹, LUO Jin-yin¹, ZHANG Shi-dong¹, LI Hong-sheng¹

¹ Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture/Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province/Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, P.R.China
² State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, P.R.China

Received:2015-12-30 Online:2016-12-01 Published:2016-12-02
Contact: LI Hong-sheng, Tel: +86-931-2164183, Fax: +86-931-2114180, E-mail: lihsheng@sina.com
About author:YANG Feng, E-mail: yangfeng@caas.cn;
Supported by:
This study was supported by the Central Public-Interest Scientific Institution Basal Research Fund, China (1610322015007), the Key Technology R&D Program of China during the 12th Five-Year Plan period (2012BAD12B03) and the Natural Science Foundation of Gansu Province, China (145RJYA311).

摘要/Abstract

Abstract: Staphylococcus aureus is the most common etiological pathogen of bovine mastitis. The resistant strains make the disease difficult to cure. The aim of this study was to characterize the genetic nature of the antimicrobial resistance in S. aureus cultured from bovine mastitis in Northwest China in 2014. A total of 44 S. aureus were isolated for antimicrobial resistance and resistance-related genes. Antimicrobial resistance was determined by disc diffusion and the corresponding resistance genes were detected by PCR. Phenotype indicated that S. aureus isolates were resistant to penicillin (84.09%), erythromycin (20.45%), tetracycline (15.91%), gentamicin (9.09%), tobramycin (6.82%), kanamycin (6.82%) and methicillin (2.27%). 9.09% of the S. aureus isolates were classified as multidrug resistant. In addition, genotypes showed that the isolates were resistant to rifampicin (100%, rpoB), penicillin (95.45%, blaZ), tetracycline (22.73%, tetK, tetM, alone or in combination), erythromycin (22.73%, ermB or ermC), gentamicin/tobramycin/kanamycin (2.27%, aacA-aphD), methicillin (2.27%, mecA) and vancomycin (2.27%, vanA). Resistance to tetracycline was attributed to the genes tetK and tetM (r=0.558, P<0.001). This study noted high-level geno- and phenotypic antimicrobial resistance in S. aureus isolates from bovine mastitis cases in Northwest China.

Key words: bovine mastitis , Staphylococcus aureus , antimicrobial resistance , phenotype , genotype

YANG Feng, WANG Qi, WANG Xu-rong, WANG Ling, LI Xin-pu, LUO Jin-yin, ZHANG Shi-dong, LI Hong-sheng. [J]. Journal of Integrative Agriculture, 2016, 15(12): 2842-2847.

YANG Feng, WANG Qi, WANG Xu-rong, WANG Ling, LI Xin-pu, LUO Jin-yin, ZHANG Shi-dong, LI Hong-sheng. Genetic characterization of antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis cases in Northwest China[J]. Journal of Integrative Agriculture, 2016, 15(12): 2842-2847.

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Genetic characterization of antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis cases in Northwest China

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